BACKGROUND & AIMS: :The dual oxidases (DUOXes) 1 and 2 are named based on their having both a domain homologous to the NADPH-oxidase of the phagocyte NADPH-oxidase gp91( phox )/NOX2 and a domain homologous to thyroid peroxidase. The DUOX1 and DUOX2 mRNAs were originally cloned from thyroid tissue, and the corresponding proteins were recognized as intricate components of the thyroid hormone synthesis process, providing hydrogen peroxide essential for the organification of iodide. The function of DUOX2 in thyroid hormonogenesis has been firmly established by linking the congenital hypothyroid phenotype "total iodide organification defect" to biallelic inactivating DUOX2 mutations. Based on the expression of both DUOXes in combination with a peroxidase in a range of different tissues and functional studies; the concept evolves that DUOX is important not only for thyroid hormonogenesis but also as an integral part of the host defense system of mucosal surfaces, participates in the control of epithelial infection, augments surface B-cell receptor signaling in lymphocytes, and is involved in generating a respiratory burst at fertilization.

背景与目标： : 双重氧化酶 (DUOXes) 1和2的命名是基于它们具有与吞噬细胞NADPH氧化酶gp91( phox )/NOX2的NADPH氧化酶同源的结构域和与甲状腺过氧化物酶同源的结构域。DUOX1和DUOX2 mrna最初是从甲状腺组织中克隆的，相应的蛋白质被认为是甲状腺激素合成过程的复杂成分，为碘化物的有机化提供了必不可少的过氧化氢。通过将先天性甲状腺功能减退表型 “总碘化物器官化缺陷” 与双等位基因失活DUOX2突变联系起来，已经牢固地确立了DUOX2在甲状腺激素发生中的功能。基于在一系列不同组织和功能研究中结合过氧化物酶的表达; 该概念演变为DUOX不仅对甲状腺激素发生很重要，而且作为粘膜表面宿主防御系统的组成部分，参与控制上皮感染，增强淋巴细胞中的表面b细胞受体信号传导，并参与受精时产生呼吸爆发。

BACKGROUND & AIMS: :The effect of immobilization on cell physiology and how this determines cell metabolic performance is an important concern for developing bioprocess. This is particularly true for genetically modified microorganisms and their genetic stability. For this reason the stability and physiological state of plasmid-bearing E. coli cells were ascertained by flow cytometry. Differences in the cellular DNA and protein content (15-20%) permit discrimination of control and plasmid-bearing cells, as well as adaptation to continuous cultivation conditions in both freely suspended and immobilized states to be monitored. Moreover, the observed metabolic burden due to maintenance and over-expression of plasmid-coded genetic material and slow cell growth in poorly-viable immobilized cells were found to be the main factors contributing to strain stabilization.

背景与目标： : 固定化对细胞生理的影响以及这如何决定细胞代谢性能是发展生物过程的重要问题。对于转基因微生物及其遗传稳定性尤其如此。因此，通过流式细胞术确定了带有质粒的大肠杆菌细胞的稳定性和生理状态。细胞DNA和蛋白质含量 (15-20%) 的差异允许区分对照和携带质粒的细胞，以及适应在自由悬浮和固定状态下的连续培养条件，以进行监测。此外，发现由于质粒编码的遗传物质的维持和过度表达以及活动性较差的固定化细胞中的细胞生长缓慢而导致的代谢负担是导致菌株稳定的主要因素。

BACKGROUND & AIMS: PURPOSE:After the Fontan procedure, children exhibit reduced peak exercise capacity, yet their submaximal exercise response remains unclear. This study sought to determine the relationship between submaximal and peak exercise capacity and physical activity in Fontan patients. METHODS:This cross-sectional study recruited 50 Fontan patients (59% males) with a median age of 9 yr (range = 6-12 yr). The median age at Fontan procedure was 2.9 yr (range = 1.6-9.1 yr). Study assessments included medical history, exercise testing, and accelerometry. RESULTS:Significantly lower submaximal oxygen consumption (V˙O2) and HR in response to a standardized workload than published values for healthy children (mean ± SD) of -1.72 ± 5.24 (P < 0.001) and -1.45 ± 1.98 (P < 0.001), respectively, suggest enhanced submaximal work efficiency in this group of patients after Fontan. Higher submaximal V˙O2 z-score was associated with higher submaximal HR z-score (P = 0.02) and lower body mass index z-score (P = 0.01). Higher V˙O2peak was associated with higher submaximal V˙O2 z-score (P < 0.01), male sex (P = 0.03), higher RER (P = 0.02), lower submaximal HR z-score (P < 0.01), and higher chronotropic responsiveness (P < 0.0001). Exercise test duration z-score was associated with lower submaximal HR z-score (P = 0.02) and higher chronotropic responsiveness (P = 0.02). CONCLUSIONS:Fontan patients exhibited a lower submaximal V˙O2 and HR responsiveness at a given workload than healthy controls did during standardized exercise testing. Thus, they may be better adapted to perform submaximal exercise. Although peak exercise capacity is limited, Fontan patients are able to perform submaximal physical activities at the same level as their healthy peers.

背景与目标：

BACKGROUND & AIMS: :Emerging evidence indicates that reactive oxygen species, especially superoxide and hydrogen peroxide, are important signaling molecules in cardiovascular cells. Their production is regulated by hormone-sensitive enzymes such as the vascular NAD(P)H oxidases, and their metabolism is coordinated by antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. Both of these reactive oxygen species serve as second messengers to activate multiple intracellular proteins and enzymes, including the epidermal growth factor receptor, c-Src, p38 mitogen-activated protein kinase, Ras, and Akt/protein kinase B. Activation of these signaling cascades and redox-sensitive transcription factors leads to induction of many genes with important functional roles in the physiology and pathophysiology of vascular cells. Thus, reactive oxygen species participate in vascular smooth muscle cell growth and migration; modulation of endothelial function, including endothelium-dependent relaxation and expression of a proinflammatory phenotype; and modification of the extracellular matrix. All of these events play important roles in vascular diseases such as hypertension and atherosclerosis, suggesting that the sources of reactive oxygen species and the signaling pathways that they modify may represent important therapeutic targets.

背景与目标： : 新兴证据表明，活性氧，尤其是超氧化物和过氧化氢，是心血管细胞中的重要信号分子。它们的产生受激素敏感酶 (例如血管NAD(P)H氧化酶) 调节，其代谢受抗氧化酶 (例如超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶) 协调。这两种活性氧都是激活多种细胞内蛋白质和酶的第二信使，包括表皮生长因子受体c-Src，p38丝裂原激活的蛋白激酶，Ras和Akt/蛋白激酶B。这些信号级联和氧化还原敏感的转录因子的激活导致诱导许多在血管细胞的生理和病理生理中具有重要功能作用的基因。因此，活性氧参与血管平滑肌细胞的生长和迁移; 调节内皮功能，包括内皮依赖性舒张和促炎表型的表达; 和细胞外基质的修饰。所有这些事件在诸如高血压和动脉粥样硬化等血管疾病中起着重要作用，表明活性氧的来源及其修饰的信号通路可能是重要的治疗靶点。

BACKGROUND & AIMS: -2

背景与目标： -2

BACKGROUND & AIMS: :An amendment to this paper has been published and can be accessed via a link at the top of the paper.

背景与目标： : 本文的修正案已经发表，可以通过本文顶部的链接进行访问。

BACKGROUND & AIMS: :Blended online and digital learning includes a variety of activities that combine engaging classroom-based education with online learning. The aim of this study is to evaluate undergraduate students' perceptions of a blended online and digital curriculum for anatomy and physiology in the nursing degree program. A quantitative methodology was used with a sample of 100 undergraduate nursing students from a single Australian University. Descriptive statistics are and presented in this article. Of the 100 participants, 90% were enrolled in the standard mode compared to 10% in a flexible mode of delivery. Results indicated that 29% of participants preferred laboratory classes as having the most impact. Participants (46%) also agreed that recorded lectures were useful, with 36% indicating that the online platform was easy to navigate going as far as wanting more online quizzes (49%). More than half of the participants (54%) acknowledged that anatomy and physiology was important for their future careers. The blended online and digital learner preferences in delivering anatomy and physiology-related courses should be adjusted in order for learning to be effective for undergraduate students in the future.

背景与目标： : 混合在线和数字学习包括各种活动，这些活动将基于课堂的引人入胜的教育与在线学习相结合。这项研究的目的是评估本科生对护理学位课程中解剖和生理学的在线和数字混合课程的看法。使用定量方法对来自一所澳大利亚大学的100名护理本科生进行了抽样。本文介绍了描述性统计。在100名参与者中，90% 名以标准模式登记，而10% 名以灵活的分娩方式登记。结果表明，29% 的参与者更喜欢实验班，因为实验班的影响最大。参与者 (46%) 还同意录制的讲座很有用，36% 表明在线平台很容易导航，甚至想要更多的在线测验 (49%)。超过一半的参与者 (54%) 承认解剖学和生理学对他们未来的职业很重要。在提供解剖学和生理学相关课程时，应调整在线和数字学习者的混合偏好，以便将来对本科生有效学习。

BACKGROUND & AIMS: :Plants have different physiological characteristics as the season changes, grazing management in compliance with plant growth and development characteristics may provide new ideas for sustainable livestock development. However, there has been little research on seasonal grazing and plants physiological responses under it. Here, we studied a typical steppe ecosystem of Inner Mongolia, with Leymus chinensis as the dominant species, in five grazing treatments: continuous grazing, seasonal grazing (which started in spring or in early and late summer), and no grazing (the control). We analyzed growth and resistance of L. chinensis in the five treatments by measuring annual primary productivity, morphological traits and various physiological processes. Compared with continuous grazing, seasonal grazing significantly alleviated grassland degradation. The plants were less affected by stress under spring grazing, with net photosynthesis and non-photochemical quenching closer to the control values and with a lower malondialdehyde content. The annual primary production of plants under grazing started in the early and late summer were 3-4 times the value under continuous grazing. Regrowth under early-summer grazing was greatly improved, and stress resistance was stronger with a higher proline content and high antioxidant enzyme activity. And nutrient accumulation at the end of the growing season such as abundant soluble sugars were transferred from aboveground tissue to the roots in September under late-summer grazing, which benefited regrowth the next year. All these physiological processes were regulated by hormonal changes. Our results highlight how plants response grazing stress in different growing seasons and suggest that seasonal grazing can improve the stress resistance and regrowth capacity of forage vegetation, and applying this knowledge can promote more sustainable grazing practices.

背景与目标： : 随着季节的变化，植物具有不同的生理特征，放牧管理符合植物生长发育特征，可能为畜牧业的可持续发展提供新思路。然而，关于季节性放牧和植物在其下的生理反应的研究很少。在这里，我们研究了内蒙古典型的草原生态系统，以羊草为优势种，在五种放牧处理中: 连续放牧，季节性放牧 (从春季开始或夏初和夏末开始) 和不放牧 (对照)。我们通过测量年度初级生产力，形态特征和各种生理过程，分析了五种处理中中华L的生长和抗性。与连续放牧相比，季节性放牧明显缓解了草地退化。春季放牧下植物受胁迫的影响较小，净光合作用和非光化学猝灭更接近对照值，丙二醛含量较低。夏初和夏末开始放牧的植物年初级产量是连续放牧的3-4倍。初夏放牧条件下的再生长大大提高，抗逆能力强，脯氨酸含量高，抗氧化酶活性高。在夏末放牧的情况下，生长季节结束时的养分积累 (例如丰富的可溶性糖) 从地上组织转移到9月的根部，这有利于第二年的再生。所有这些生理过程都受到激素变化的调节。我们的结果强调了植物在不同生长季节如何应对放牧胁迫，并表明季节性放牧可以提高牧草植被的抗逆性和再生能力，应用这些知识可以促进更可持续的放牧实践。

BACKGROUND & AIMS: :Cellular function requires coordination between different organelles and metabolic cues. Mitochondria and lysosomes are essential for cellular metabolism as major contributors of chemical energy and building blocks. It is therefore pivotal for cellular function to coordinate the metabolic roles of mitochondria and lysosomes. However, these organelles do more than metabolism, given their function as fundamental signaling platforms in the cell that regulate many key processes such as autophagy, proliferation, and cell death. Mechanisms of crosstalk between mitochondria and lysosomes are discussed, both under physiological conditions and in diseases that affect these organelles.

背景与目标： : 细胞功能需要不同细胞器和代谢线索之间的协调。线粒体和溶酶体对于细胞代谢至关重要，是化学能和组成部分的主要贡献者。因此，协调线粒体和溶酶体的代谢作用对于细胞功能至关重要。然而，这些细胞器不仅仅是代谢，因为它们作为细胞中的基本信号平台，调节许多关键过程，如自噬、增殖和细胞死亡。讨论了在生理条件下和影响这些细胞器的疾病中线粒体和溶酶体之间的串扰机制。

BACKGROUND & AIMS: :Limbic epilepsy is a chronic condition associated with a broad zone of seizure onset and pathology. Studies have focused mainly on the hippocampus, but there are indications that changes occur in other regions of the limbic system. This study used in vitro intracellular recording and histology to examine alterations to the physiology and anatomy of the basal nucleus of the amygdala in a rat model of chronic limbic epilepsy characterized by spontaneously recurring seizures. Epileptic pyramidal neuron responses evoked by stria terminalis stimulation revealed hyperexcitability characterized by multiple action potential bursts and no evident inhibitory potentials. In contrast, no hyperexcitability was observed in amygdalar neurons from kindled (included as a control for seizure activity) or control rats. Blockade of ionotropic glutamate receptors unmasked inhibitory postsynaptic potentials in epileptic pyramidal neurons. Control, kindled and epileptic inhibitory potentials were predominantly biphasic, with fast and slow components, but a few cells exhibited only the fast component (2/12 in controls, 0/3 in kindled, 3/10 in epileptic). Epileptic fast inhibitory potentials had a more rapid onset and shorter duration than control and kindled. Approximately 40% of control neurons exhibited spontaneous inhibitory potentials; no spontaneous inhibitory potentials were observed in neurons from kindled or epileptic rats. A preliminary histological examination revealed no gross alterations in the basal amygdala from epileptic animals. These results extend previous findings from this laboratory that hyperexcitability is found in multiple epileptic limbic regions and may be secondary to multiple alterations in excitatory and inhibitory efficacy. Because there were no differences between control and kindled animals, the changes observed in the epileptic animals are unlikely to be secondary to recurrent seizures.

背景与目标： : 边缘癫痫是一种慢性疾病，与癫痫发作和病理的广泛区域相关。研究主要集中在海马体上，但有迹象表明边缘系统的其他区域也发生了变化。这项研究使用体外细胞内记录和组织学检查了以自发性癫痫为特征的慢性边缘癫痫大鼠模型中杏仁核基底核的生理和解剖学变化。纹状体刺激引起的癫痫锥体神经元反应显示出过度兴奋，其特征是多动作电位爆发，没有明显的抑制电位。相反，在点燃的杏仁核神经元 (包括作为癫痫发作活动的对照) 或对照大鼠中未观察到过度兴奋。离子型谷氨酸受体的阻断揭示了癫痫锥体神经元的抑制性突触后电位。对照，点燃和癫痫抑制电位主要是双相的，具有快速和缓慢成分，但少数细胞仅表现出快速成分 (对照2/12，点燃0/3，癫痫3/10)。与对照和点燃相比，癫痫快速抑制电位的发作更快，持续时间更短。大约40% 的对照神经元表现出自发抑制电位; 在点燃或癫痫大鼠的神经元中未观察到自发抑制电位。初步的组织学检查显示，癫痫动物的基底杏仁核没有明显改变。这些结果扩展了该实验室先前的发现，即在多个癫痫边缘区域发现了过度兴奋，并且可能继发于兴奋性和抑制性功效的多种改变。由于对照动物和点燃动物之间没有差异，因此在癫痫动物中观察到的变化不太可能继发于反复发作。

BACKGROUND & AIMS: :The stoichiometric limit to the biomass yield (maximal assimilation of the carbon source) is determined by the amount of CO2 lost in anabolism and the amount of carbon source required for generation of NADPH. This stoichiometric limit may be reached when yeasts utilize formate as an additional energy source. Factors affecting the biomass yield on single substrates are discussed under the following headings: Energy requirement for biomass formation (YATP). YATP depends strongly on the nature of the carbon source. Cell composition. The macroscopic composition of the biomass, and in particular the protein content, has a considerable effect on the ATP requirement for biomass formation. Hence, determination of for instance the protein content of biomass is relevant in studies on bioenergetics. Transport of the carbon source. Active (i.e. energy-requiring) transport, which occurs for a number of sugars and polyols, may contribute significantly to the calculated theoretical ATP requirement for biomass formation. P/O-ratio. The efficiency of mitochondrial energy generation has a strong effect on the cell yield. The P/O-ratio is determined to a major extent by the number of proton-translocating sites in the mitochondrial respiratory chain. Maintenance and environmental factors. Factors such as osmotic stress, heavy metals, oxygen and carbon dioxide pressures, temperature and pH affect the yield of yeasts. Various mechanisms may be involved, often affecting the maintenance energy requirement. Metabolites such as ethanol and weak acids. Ethanol increases the permeability of the plasma membrane, whereas weak acids can act as proton conductors. Energy content of the growth substrate. It has often been attempted in the literature to predict the biomass yield by correlating the energy content of the carbon source (represented by the degree of reduction) to the biomass yield or the percentage assimilation of the carbon source. An analysis of biomass yields of Candida utilis on a large number of carbon sources indicates that the biomass yield is mainly determined by the biochemical pathways leading to biomass formation, rather than by the energy content of the substrate.

背景与目标： : 生物量产量的化学计量极限 (碳源的最大同化) 取决于合成代谢中损失的CO2量和产生NADPH所需的碳源量。当酵母利用甲酸作为附加能源时，可能会达到该化学计量极限。在以下标题下讨论了影响单基质生物量产量的因素: 生物质形成的能量需求 (YATP)。YATP在很大程度上取决于碳源的性质。细胞组成。生物质的宏观组成，特别是蛋白质含量，对生物质形成的ATP需求具有相当大的影响。因此，例如确定生物质的蛋白质含量与生物能学研究有关。碳源的运输。对于许多糖和多元醇发生的主动 (即需要能量的) 运输，可能对计算出的生物质形成的理论ATP需求有很大贡献。市盈率。线粒体能量产生的效率对细胞产量有很大影响。P/O比在很大程度上取决于线粒体呼吸链中质子转移位点的数量。维护和环境因素。渗透胁迫，重金属，氧气和二氧化碳压力，温度和pH等因素影响酵母的产量。可能涉及各种机制，通常会影响维护能量需求。代谢产物如乙醇和弱酸。乙醇增加了质膜的渗透性，而弱酸可以充当质子导体。生长基质的能量含量。文献中经常尝试通过将碳源的能量含量 (由还原程度表示) 与生物质产量或碳源的同化百分比相关联来预测生物质产量。对大量碳源上的假丝酵母的生物量产量的分析表明，生物量产量主要取决于导致生物量形成的生化途径，而不是取决于底物的能量含量。

BACKGROUND & AIMS: :The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cav channel and rectifying Kv channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.

背景与目标： : anamniote侧线系统，包括机械感觉神经管和电感觉壶腹器官，是研究不同器官和细胞类型的发育和进化多样化的有用模型。斑马鱼神经肥大的发育越来越被人们所了解，但是斑马鱼和非洲爪蟾都不是电感受性的，我们对壶腹器官发育的分子理解还很初步。我们已经使用RNA-seq从晚幼虫paddlefish (Polyodon spathula) 产生了一条富含侧线的基因集。子集的验证揭示了对毛细胞发育至关重要的转录因子基因的发育中的壶腹器官中的表达，以及毛细胞带状突触中谷氨酸释放必不可少的基因，表明非硬骨膜电感受器与毛细胞之间的密切发育，生理和进化联系。我们确定了壶腹器官特异性的前神经转录因子，并根据滑冰 (软骨鱼) 壶腹器官电生理预测了电压感应L型Cav通道和整流Kv通道。总的来说，我们的结果阐明了壶腹器官的发育，生理和进化。

BACKGROUND & AIMS: :Cannabis sativa has been known, used, and misused by mankind for centuries, and yet only over the last two decades has research stemming from the chemical constituents specific to this plant, the cannabinoids, started to provide fundamental insights into animal physiology and pathology, resulting in the development of new therapeutics. The discovery of the endocannabinoid system, and its targeting with two new pharmaceutical preparations now on the market in several countries, represent the most recent example of how studies on medicinal plants and on the mechanism of their biological effects can reveal, through a chain of breakthroughs, new systems of endogenous signals and physiological phenomena that can become the source of novel strategies for unmet therapeutic challenges.

背景与目标： : 大麻已经被人类认识，使用和滥用了几个世纪，但仅在过去的二十年中，来自该植物特有的化学成分大麻素的研究开始提供对动物生理学和病理学的基本见解，导致新疗法的发展。内源性大麻素系统的发现及其以目前在几个国家市场上的两种新药物制剂为目标，代表了对药用植物及其生物效应机制的研究如何通过一系列突破揭示的最新例子，内源性信号和生理现象的新系统，可以成为应对未解决的治疗挑战的新策略的来源。

BACKGROUND & AIMS: PURPOSE:This review presents a perspective on the expansive literature on rowing. METHODS:The PubMed database was searched for the most relevant literature, while some information was obtained from books. RESULTS:Following the life span of former rowers paved the way to advocate exercise for health promotion. Rowing involves almost all muscles during the stroke and competition requires a large oxygen uptake, which is challenged by the pulmonary diffusion capacity and restriction in blood flow to the muscles. Unique training adaptations allow for simultaneous engagement of the legs in the relatively slow movement of the rowing stroke that, therefore, involves primarily slow-twitch muscle fibres. Like other sport activities, rowing is associated with adaptation not only of the heart, including both increased internal diameters and myocardial size, but also skeletal muscles with hypertrophy of especially slow-twitch muscle fibres. The high metabolic requirement of intense rowing reduces blood pH and, thereby, arterial oxygen saturation decreases as arterial oxygen tension becomes affected. CONCLUSION:Competitive rowing challenges most systems in the body including pulmonary function and circulatory control with implication for cerebral blood flow and neuromuscular activation. Thus, the physiology of rowing is complex, but it obviously favours large individuals with arms and legs that allow the development of a long stroke. Present inquiries include the development of an appropriately large cardiac output despite the Valsalva-like manoeuvre associated with the stroke, and the remarkable ability of the brain to maintain motor control and metabolism despite marked reductions in cerebral blood flow and oxygenation.

背景与目标：

BACKGROUND & AIMS: :Articular cartilage serves as a load-bearing elastic material that is responsible for the frictionless movement of the surfaces of articulating joints. Its ability to undergo reversible deformation depends on its structural organization, including the specific arrangement of the matrix macromolecules and the chondrocytes. Interactions between the matrix and chondrocytes are responsible for the biological and mechanical properties of articular cartilage and enable it to respond by effecting a balance between anabolism and catabolism as well as continual internal remodeling. Age-related changes in the function of chondrocytes may contribute to the initiation and progression of osteoarthritis.

背景与目标： : 关节软骨是一种承重的弹性材料，负责关节关节表面的无摩擦运动。它进行可逆变形的能力取决于其结构组织，包括基质大分子和软骨细胞的特定排列。基质和软骨细胞之间的相互作用负责关节软骨的生物学和机械特性，并通过影响合成代谢和分解代谢之间的平衡以及持续的内部重塑来使其做出反应。软骨细胞功能的年龄相关变化可能有助于骨关节炎的发生和发展。