BACKGROUND & AIMS:
:A neutral polysaccharide (PZMP1) with a molecular weight of 16.97 kDa was isolated from Ziziphus Jujuba cv. Muzao with water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. PZMP1 was structurally characterized with high-performance gel-permeation chromatography, gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, NMR spectroscopy, X-ray diffraction, scanning electron microscopy, and atomic force microscopy. The results show that PZMP1 is a homogeneous heteropolysaccharide composed of arabinose, galactose, glucose, mannose, and xylose in a molar percentage of 17.36: 3.29:2.68:1.05:1.00. The majorly backbone of PZMP1 is composed of 1,3,5-linked Araf, 1,3-linked Araf, 1,5-linked Araf, 1,4-linked Glcp, and 1-linked Araf, 1-linked Glcp. According to an NMR analysis, the repeating unit of PZMP1 is a linear backbone with branches at positions O-3, and O-5. PZMP1 also displays effective dose-dependent hypolipidemic activity, suggesting that it is a natural hypolipidemic agent, which could contribute to the functional food qualities of jujube fruit.
背景与目标:
:从Zipziphus Jujuba cv中分离出分子量为16.97kDa的中性多糖(PZMP1)。用水提取木枣,乙醇沉淀,并用DEAE-Sepharose Fast Flow和Sephacryl S-300柱色谱法纯化。通过高效凝胶渗透色谱,气相色谱,傅立叶变换红外光谱,甲基化分析,NMR光谱,X射线衍射,扫描电子显微镜和原子力显微镜对PZMP1进行结构表征。结果表明,PZMP1是一种均一的杂多糖,由阿拉伯糖,半乳糖,葡萄糖,甘露糖和木糖组成,摩尔百分比为17.36:3.29:2.68:1.05:1.00。 PZMP1的主要骨架由1,3,5-连接的Araf,1,3-连接的Araf,1,5-连接的Araf,1,4-连接的Glcp和1-连接的Araf,1-连接的Glcp组成。根据NMR分析,PZMP1的重复单元是在位置O-3和O-5处具有分支的线性骨架。 PZMP1还显示出有效的剂量依赖性降血脂活性,表明它是一种天然的降血脂药,可有助于枣果的功能食品品质。