BACKGROUND & AIMS:
:The aim of the present study was to evaluate the hypolipidemic and antioxidant potential of saffron and its active constituent, crocin, in hyperlipidemic rats. The animals fed either with normal fat diet or high fat diet were administered orally saffron (25, 50, and 100 mg/kg) or crocin (4.84, 9.69, and 19.38 mg/kg) in their respective groups for five consecutive days. Biochemical estimations of triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), alkaline phosphatase (ALP), aspartate transaminase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), glutathione peroxidase enzyme activity (GSHPx), total glutathione (GSH), and oxidized glutathione (GSSG) in serum and superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive species (TBARS), ferric reducing/antioxidant power (FRAP), and total sulfhydryl (SH) groups in liver tissue homogenate were carried out. Both saffron and crocin were effective in decreasing the elevated levels of TG, TC, ALP, AST, ALT, MDA, GSHPx, GSH, and GSSG in serum and increasing SOD, CAT, FRAP, and SH values in liver tissue with reduction in TBARS. The saffron was found to be superior to crocin indicating the involvement of other potential constituents of saffron apart from crocin for its synergistic behavior of quenching the free radicals and ameliorating the damages of hyperlipidemia.
背景与目标:
:本研究的目的是评估高脂血症大鼠中藏红花及其活性成分藏红花素的降血脂和抗氧化能力。饲喂正常脂肪饮食或高脂肪饮食的动物连续五天分别口服番红花(25、50和100 mg / kg)或番红花(4.84、9.69和19.38 mg / kg)。甘油三酸酯(TG),总胆固醇(TC),高密度脂蛋白(HDL),低密度脂蛋白(LDL),碱性磷酸酶(ALP),天冬氨酸转氨酶(AST),丙氨酸氨基转移酶(ALT),丙二醛( MDA),血清和超氧化物歧化酶(SOD),过氧化氢酶(CAT),硫代巴比妥酸反应性物种(TBARS)中的谷胱甘肽过氧化物酶活性(GSHPx),总谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG),铁还原/抗氧化能力(FRAP)和肝组织匀浆中的总巯基(SH)基团。藏红花和藏红花均可有效降低血清中TG,TC,ALP,AST,ALT,MDA,GSHPx,GSH和GSSG的升高水平,并通过降低TBARS来提高肝脏组织中的SOD,CAT,FRAP和SH值。发现藏红花优于番红花,这表明藏红花除了藏红花素还参与了藏红花的其他潜在成分,因为它具有协同作用,可以淬灭自由基并减轻高脂血症的损害。