BACKGROUND & AIMS:
:Although poly(glycerol sebacate) (PGS) has enjoyed great success in soft tissue engineering, it remains challenging to fabricate PGS fibers. In this study, coaxial electrospinning, in which polylactide (PLA) was used to confine and draw PGS prepolymer, was used to fabricate PGS fibrous membranes. Specifically, effects of adding poly(ethylene oxide) (PEO), which was removed prior to curing, in the shell were investigated. Transmission and scanning electron microscopy were used to confirm core-shell structure and morphology of fibers, respectively. Both the removal of PEO or PLA in the shell and the efficacy of PGS curing were verified by Fourier transform infrared spectroscopy and differential scanning calorimetry. Mechanical properties of the membranes with different shell and core contents were examined. We found that the addition of PEO to the shell reduced Young׳s modulus of the resulting cured membrane and increased its elongation at break significantly, the latter indicating better PGS curing. Moreover, with the addition of PEO, increasing PGS prepolymer concentration further increased the elongation at break and appeared to enhance the structural integrity of fibers; PGS fibrous membranes (with no PLA shell) were thus successfully fabricated after the removal of PLA. The Young׳s modulus of the PGS fibrous membrane was ~0.47MPa, which is similar to that of PGS solid sheets and some soft tissues. Finally, the cytocompatibility of the electrospun membranes was validated by Alamar blue and LDH assays.
背景与目标:
:尽管聚癸二酸甘油酯(PGS)在软组织工程中获得了巨大的成功,但制造PGS纤维仍然具有挑战性。在这项研究中,使用聚乳酸(PLA)限制和拉伸PGS预聚物的同轴电纺丝用于制造PGS纤维膜。具体地,研究了在壳中添加在固化之前被除去的聚环氧乙烷(PEO)的效果。透射电子显微镜和扫描电子显微镜分别用于确认纤维的核-壳结构和形态。通过傅里叶变换红外光谱和差示扫描量热法验证了壳中PEO或PLA的去除以及PGS固化的有效性。检查了具有不同壳和芯含量的膜的机械性能。我们发现,在壳中添加PEO会降低所得固化膜的杨氏模量,并显着提高其断裂伸长率,后者表明PGS固化效果更好。而且,通过添加PEO,增加的PGS预聚物浓度进一步增加了断裂伸长率,并且似乎增强了纤维的结构完整性。因此,在除去PLA后成功地制备了PGS纤维膜(无PLA壳)。 PGS纤维膜的杨氏模量约为0.47MPa,与PGS固体薄片和一些软组织的杨氏模量相似。最后,通过Alamar蓝和LDH分析验证了电纺膜的细胞相容性。