This study presents a bilayer structure as a skin scaffold comprised of an electrospun sheet layer made of polycaprolactone and polyvinil alcohol and a porous hydrogel layer made of chitosan and gelatin. The hydrogel layer was fabricated by employing the freeze-gelation technique. The bilayer structure was achieved by pouring the hydrogel solution on the electrospun sheet at the bottom of a mold followed by the freeze-gelation technique to obtain a porous structure in the hydrogel. The hydrogel and hydrogel-electrospun samples were characterized by scanning electron microscopy, swelling, tensile strength, in vitro and in vivo analyses. From a mechanical strength standpoint, the combination of hydrogel and electrospun layers produced 110 and 133% increases in mean tensile strength and elastic modulus values, respectively compared to the single layer hydrogel sample. While the results of swelling and cell proliferation tests did not yield significant differences between the two groups, the bilayer scaffold performed better considering the results of the in vivo analysis. It was also seen that there was a good connection between the two layers in the bilayer scaffold, making the necessary manipulations and clinical handling convenient.

译文

:这项研究提出了一种双层结构,作为皮肤支架,由聚己内酯和聚乙烯醇制成的电纺片层和壳聚糖和明胶制成的多孔水凝胶层组成。水凝胶层是通过采用冷冻凝胶技术制备的。通过将水凝胶溶液倒在模具底部的电纺片上,然后进行冷冻凝胶化技术以获得水凝胶中的多孔结构,从而获得双层结构。通过扫描电子显微镜,溶胀,拉伸强度,体外和体内分析来表征水凝胶和水凝胶电纺丝样品。从机械强度的观点来看,与单层水凝胶样品相比,水凝胶层和电纺丝层的组合产生的平均抗张强度和弹性模量值分别提高了110%和133%。尽管两组之间的肿胀和细胞增殖测试结果没有显着差异,但考虑到体内分析的结果,双层支架的性能更好。还发现双层支架中的两层之间有良好的连接,从而使必要的操作和临床处理变得方便。

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