BACKGROUND & AIMS:
:The purpose of this research is to establish an injectable hydrogel encapsulating copper sulfide (CuS) nanodots for photothermal therapy against cancer. The CuS nanodots were prepared by one-pot synthesis, and the thermosensitive Pluronic F127 was used as the hydrogel matrix. The CuS nanodots and the hydrogel were characterized by morphous, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation, and rheology features. The effects of the CuS nanodots and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention, photothermal efficacy, therapeutic effects, and systemic toxicity of the hydrogel were assessed in tumor bearing mouse model. The CuS nanodots with a diameter of about 8 nm exhibited satisfying serum stability, photoheat conversion ability, and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanodot-loaded hydrogel shows a phase transition at body temperature and, as a result, a long retention in vivo. The photothermal-agent-embedded hydrogel played a promising photothermal therapeutic effect in the tumor bearing mouse model with low systemic toxicity after peritumoral administration.
背景与目标:
: 这项研究的目的是建立一种可注射的水凝胶,该水凝胶封装了硫化铜 (CuS) 纳米点,用于光热治疗癌症。通过一锅法合成制备了CuS纳米点,并将热敏Pluronic F127用作水凝胶基质。Cu纳米点和水凝胶的特征在于形态,粒径,血清稳定性,重复808 nm激光照射后的光热性能和流变学特征。定性和定量评估了CuS纳米点和水凝胶在4T1小鼠乳腺癌细胞中的作用。在荷瘤小鼠模型中评估了水凝胶的保留,光热功效,治疗效果和全身毒性。直径约为8 nm的CuS纳米点表现出令人满意的血清稳定性,光热转换能力和重复激光曝光稳定性。水凝胶封装不会对光热剂的上述特征产生负面影响。装有纳米点的水凝胶在体温下显示出相变,因此在体内保留时间长。包埋的光热剂水凝胶在瘤周给药后具有低全身毒性的荷瘤小鼠模型中发挥了有希望的光热治疗作用。