Thermosensitive injectable chitosan hydrogels can be formed by neutralization of acidic chitosan solutions with sodium betaglycerophosphate (Na-β-GP) coupled with increasing temperature to body temperature. Such hydrogels have been considered for applications in bone regeneration. In this study, chitosan hydrogels were enriched with glycerol and the enzyme alkaline phosphatase (ALP) with a view to improving their suitability as materials for bone tissue engineering. Mineral formation was confirmed by infrared spectroscopy (FTIR) and increases in the mass fraction of the hydrogel not consisting of water. Incorporation of ALP in hydrogels followed by incubation in a solution containing calcium ions and glycerophosphate, a substrate for ALP, led to formation of calcium phosphate within the hydrogel. MG-63 osteoblast-like cells were cultivated in eluates from hydrogels containing ALP and without ALP at different dilutions and directly on the hydrogel samples. Hydrogels containing ALP exhibited superior cytocompatibility to ALP-free hydrogels. These results pave the way for the use of glycerol- and ALP-enriched hydrogels in bone regeneration.

译文

热敏性可注射壳聚糖水凝胶可以通过用 β 甘油磷酸钠 (Na-β-GP) 中和酸性壳聚糖溶液并增加温度至体温来形成。这种水凝胶已被考虑用于骨再生。在这项研究中,壳聚糖水凝胶富含甘油和碱性磷酸酶 (ALP),以提高其作为骨组织工程材料的适用性。通过红外光谱 (FTIR) 确认了矿物的形成,并且不包含水的水凝胶的质量分数增加。将ALP掺入水凝胶中,然后在含有钙离子和甘油磷酸 (ALP的底物) 的溶液中孵育,导致在水凝胶中形成磷酸钙。MG-63成骨细胞样细胞在洗脱液中培养,从含有ALP和不含ALP的水凝胶在不同稀释度下,并直接在水凝胶样品上培养。含有ALP的水凝胶显示出优于无ALP水凝胶的细胞相容性。这些结果为在骨再生中使用富含甘油和ALP的水凝胶铺平了道路。

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