BACKGROUND & AIMS:
BACKGROUND:Ophiocordyceps sinensis is an important traditional Chinese medicine for its comprehensive active ingredients, such as cordycepin, cordycepic acid, and Cordyceps polysaccharide. O. sinensis zjut, a special strain isolated from O. sinensis, has similar pharmacological functions to wild O. sinensis. Currently, O. sinensis with artificial cultivation has been widely studied, but systematic fundamental research at protein levels has not been determined.
RESULTS:Proteomes of O. sinensis zjut at different culture periods (growth period, 3rd day; pre-stable period, 6th day; and stable period, 9th day) were relatively quantified by relative isotope markers and absolute quantitative technology. In total, 4005 proteins were obtained and further annotated with Gene Ontology, Kyoto Encyclopedia of Genes and Genomes database. Based on the result of the annotations, metabolic pathways of active ingredients, amino acids and fatty acid were constructed, and the related enzymes were exhibited. Subsequently, comparative proteomics of O. sinensis zjut identified the differentially expressed proteins (DEPs) by growth in different culture periods, to find the important proteins involved in metabolic pathways of active ingredients. 605 DEPs between 6d-VS-3d, 1188 DEPs between 9d-VS-3d, and 428 DEPs between 9d-VS-6d were obtained, respectively.
CONCLUSION:This work provided scientific basis to study protein profile and comparison of protein expression levels of O. sinensis zjut, and it will be helpful for metabolic engineering works to active ingredients for exploration, application and improvement of this fungus.
背景与目标:
背景:中华O虫草是虫草素,虫草酸,虫草多糖等综合活性成分,是一种重要的中药。 O. sinensis zjut是一种从O. sinensis分离的特殊菌株,具有与野生O. sinensis相似的药理功能。目前,对人工栽培的中华稻进行了广泛的研究,但尚未确定蛋白质水平的系统基础研究。
结果:通过相对同位素标记和绝对定量技术对不同培养时期(生长期第3天;稳定期第6天;稳定期第9天)中华稻的蛋白质组进行了相对定量。总共获得了4005种蛋白质,并用“基因本体论”,“京都基因百科全书和基因组”数据库进一步注释。根据注释结果,构建了活性成分,氨基酸和脂肪酸的代谢途径,并展示了相关的酶。随后,中华绒螯蟹的比较蛋白质组学通过在不同培养时期的生长鉴定出差异表达的蛋白质(DEPs),以发现参与活性成分代谢途径的重要蛋白质。获得了6d-VS-3d之间的605 DEP,9d-VS-3d之间的1188 DEP和9d-VS-6d之间的428 DEP。
结论:这项研究为研究中华稻的蛋白质谱和比较蛋白质表达水平提供了科学依据,将有助于该真菌的有效成分的代谢工程研究,探索,应用和改良。