Ophiocordyceps sinensis (Berk.) Sacc. is one of the most well-known fungi in traditional Chinese medicine and is attracting attention because of its nutritious and medicinal properties. The present study aimed to produce a proteomic map to identify common O. sinensis proteins. The caterpillar body and stroma of O. sinensis collected from five locations and four fungal specimens of similar appearance were examined by two-dimensional electrophoresis (2-DE). Five proteins were identified using MALDI-TOF--TOF/MS, and the 2-DE identification pattern was provided. OCS_04585 and β-lactamase domain-containing protein, the two abundant and characteristic proteins, were separated and purified using liquid-phase isoelectric focusing. The products were high-quality materials that can be used for future protein-function studies and immunoassay development.

译文

中华phi是中草药中最著名的真菌之一,由于其营养和药用特性而引起了人们的关注。本研究旨在产生一个蛋白质组图谱,以鉴定常见的中华稻草蛋白。通过二维电泳(2-DE)检查了从五个位置收集的中华稻草的毛虫体和间质以及四个相似外观的真菌标本。使用MALDI-TOF--TOF / MS鉴定了5种蛋白质,并提供了2-DE鉴定模式。使用液相等电聚焦法分离并纯化了OCS_04585和含β-内酰胺酶结构域的蛋白质(这两个丰富且特征性的蛋白质)。这些产品是高质量的材料,可用于将来的蛋白质功能研究和免疫测定开发。

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