BACKGROUND & AIMS:
:Based on colloidal gold and broad-spectrum monoclonal antibody that binds to zeranol and its five analogues with high sensitivity, a lateral flow immunochromatographic assay (LFIA) in a competitive format was developed to specifically determine residues of zeranol, an illegal growth promoter in livestock. In this study, the assay had high sensitivity and was broad-spectrum only for zeranol and its five analogues, and the results were obtained within 10 min without needing sophisticated procedures. The cutoff values for zeranol and its five analogues were 10 ng/mL, and the IC50 values for zeranol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol and zearalenone were 1.250, 1.800, 1.775, 1.225, 1.709 and 1.319 ng/mL, respectively. The recovery rates were ranged from 85.6 to 93.9%, with the coefficient of variations less than 12.4%. The results demonstrated that the LFIA could be used for rapid, simultaneous, semi-quantitative and quantitative detection of residues of zeranol and its five analogous in milk.
背景与目标:
:基于胶体金和广谱单克隆抗体,它们以高灵敏度结合至沙仑醇及其五种类似物,开发了一种竞争性形式的侧流免疫色谱测定法(LFIA),用于特异性测定家畜非法生长促进剂沙仑醇的残留量。在这项研究中,该测定法具有很高的灵敏度,并且仅对麦醇及其五种类似物具有广谱性,并且无需复杂的程序即可在10分钟内获得结果。 zeranol及其5个类似物的截断值为10 ng / mL,zeranol,β-玉米赤霉烯醇,玉米赤霉烯酮,α-玉米赤霉烯醇,β-玉米赤霉烯醇和玉米赤霉烯酮的IC50值为1.250、1.800、1.775、1.225、1.709和1.319 ng / mL。回收率介于85.6%至93.9%之间,变异系数小于12.4%。结果表明,LFIA可用于快速,同时,半定量和定量检测牛奶中的麦角甾醇及其五种类似物的残留。