PURPOSE:This study aimed to determine the efficacy of thermotherapy in the presence of gold nanoparticles (GNPs) and microwave (MW) radiation at a frequency of 2450 MHz on the survival of Leishmania major promastigotes and amastigotes. MATERIALS AND METHODS:L. major promastigotes (strain MRHO/IR/75/ER) were cultured in RPMI-1640 medium supplemented with foetal bovine serum and antibiotic. The promastigotes were incubated with GNPs for 2 h. After washing, thermotherapy was performed by MW irradiation. After 48 h the promastigote survival rate was assessed using Alamar Blue assay. In the second part of the study, after culture and proliferation of J744 cells, the infected macrophages were incubated with the GNPs and were inserted under MW irradiation. After 24 h, the number of amastigotes in the macrophages was determined after Giemsa staining by a light microscope. RESULT:Increased exposure time of the microwave to the parasites in the presence of GNPs induced a significant decline in promastigotes survival rate in comparison to similar samples without GNPs. The least survival of amastigotes was also recorded in the groups containing GNPs. The presence of GNPs during MW irradiation was more lethal for promastigotes and amastigotes in comparison to MW alone. CONCLUSION:Thermotherapy using MW radiation in the presence of GNPs may be proposed as a new approach to treat leishmaniasis in future studies.

译文

目的:本研究旨在确定在2450 MHz频率存在金纳米颗粒(GNP)和微波(MW)辐射的情况下,热疗法对利什曼原虫主要前鞭毛体和拟吻足动物的存活的功效。
材料与方法:L.在补充胎牛血清和抗生素的RPMI-1640培养基中培养主要的前鞭毛体(菌株MRHO / IR / 75 / ER)。将前鞭毛体与GNP一起温育2小时。洗涤后,通过MW照射进行热疗。 48小时后,使用Alamar Blue分析法评估前鞭毛体的存活率。在研究的第二部分中,在培养和增殖J744细胞后,将感染的巨噬细胞与GNP一起孵育,并在MW辐射下插入。 24小时后,在吉姆萨染色后,用光学显微镜测定巨噬细胞中变形虫的数目。
结果:与不含GNP的类似样品相比,存在GNP的情况下微波暴露于寄生虫的时间增加,导致前鞭毛体成活率显着下降。在含有GNP的组中,amastigotes的存活率也最低。与单独的MW相比,MW照射期间GNPs的存在对前鞭毛体和扁桃体更具致死性。
结论:在未来的研究中,在存在GNPs的情况下使用MW辐射进行热疗可能是治疗利什曼病的一种新方法。

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