In-vitro metabolite and drug detection rely on designed materials-based analytical platforms, which are universally used in biomedical research and clinical practice. However, metabolic analysis in bio-samples needs tedious sample preparation, due to the sample complexity and low molecular abundance. A further challenge is to construct diagnostic tools. Herein, we developed a platform using silver nanoshells. We synthesized SiO2@Ag with tunable shell structures by multi-cycled silver mirror reactions. Optimized nanoshells achieved direct laser desorption/ionization mass spectrometry in 0.5 μL of bio-fluids. We applied these nanoshells for disease diagnosis and therapeutic evaluation. We identified patients with postoperative brain infection through daily monitoring and glucose quantitation in cerebrospinal fluid. We measured drug distribution in blood and cerebrospinal fluid systems and validated the function of blood-brain/cerebrospinal fluid-barriers for pharmacokinetics. Our work sheds light on the design of materials for advanced metabolic analysis and precision diagnostics.Preparation of samples for diagnosis can affect the detection of biomarkers and metabolites. Here, the authors use a silver nanoparticle plasmonics approach for the detection of biomarkers in patients as well as investigate the distribution of drugs in serum and cerebral spinal fluid.

译文

:体外代谢物和药物检测依赖于基于材料的设计分析平台,这些平台普遍用于生物医学研究和临床实践。但是,由于样品的复杂性和低分子丰度,生物样品中的代谢分析需要繁琐的样品制备。另一个挑战是构建诊断工具。在这里,我们开发了一个使用银纳米壳的平台。我们通过多周期银镜反应合成了具有可调壳结构的SiO2 @ Ag。优化的纳米壳可在0.5μL的生物流体中实现直接激光解吸/电离质谱。我们将这些纳米壳用于疾病诊断和治疗评估。我们通过每日监测和脑脊液中的葡萄糖定量来确定术后脑部感染的患者。我们测量了血液和脑脊液系统中的药物分布,并验证了血脑/脑脊液屏障对药代动力学的作用。我们的工作为高级代谢分析和精确诊断的材料设计提供了启发,用于诊断的样品制备可能会影响生物标志物和代谢物的检测。在这里,作者使用银纳米粒子等离激元方法检测患者体内的生物标志物,并研究药物在血清和脑脊髓液中的分布。

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