BACKGROUND & AIMS:
:In our search for novel anaerobes with potential carbohydrate polymers degrading activity, we have isolated a xylan-degrading bacterial strain SYSU GA17129T from an anammox bacteria dominant wastewater treatment plant. Phylogenetic analysis of the 16S rRNA gene sequence indicated the strain SYSU GA17129T belong to the order Clostridiales and shared highest sequence identity with Caldicoprobacter faecalis DSM 20678T (89.9%). The strain was thermophilic, obligately anaerobic, non-motile and rod shaped. Optimum growth of the strain was observed at 45 °C, pH 8.0 and in the presence of 0.5% NaCl (w/v). The chemotaxonomic features of the strain SYSU GA17129T comprised of C14:0 FAME, iso-C15:0 FAME and C16:0 FAME as the major fatty acids (>10%), diphosphatidylglycerol, phosphatidylinositol mannoside, an unidentified phospholipid, three unidentified polar lipids and two unidentified glycolipids as its polar lipids, and meso-diaminopimelic acid (meso-DAP) as the diamino acid in peptidoglycan. The G + C content of the genomic DNA was 35.9%. The strain could be distinguished from other defined families within the order Clostridiales by the differences in phenotypic and physiological characteristics, distinct phylogenetic lineage in 16S rRNA gene- and genome-based phylogenies and low genomic relatedness index. Based on these distinguishing properties, strain SYSU GA17129T is proposed to represent a new species of a new genus Xylanivirga thermophila gen. nov., sp. nov., within a new family Xylanivirgaceae fam. nov. The type species of the new taxon is SYSU GA17129T (=KCTC 15754T = CGMCC 1.5282T). This strain is characterized within the order Clostridiales, class Clostridia of the phylum Firmicutes.
背景与目标:
: 在寻找具有潜在碳水化合物聚合物降解活性的新型厌氧菌时,我们从anammox细菌优势废水处理厂中分离出了降解木聚糖的细菌菌株SYSU GA17129T。对16S rRNA基因序列的系统发育分析表明,SYSU GA17129T菌株属于梭状芽胞杆菌的目的,并且与粪钙菌DSM 20678T (89.9%) 具有最高的序列同一性。该菌株是嗜热的,专性厌氧的,不活动的和杆状的。在45 ℃ 、pH 8.0和0.5% NaCl (w/v) 存在下观察到菌株的最佳生长。SYSU GA17129T菌株的化学分类学特征包括C14:0 FAME,iso-C15:0 FAME和C16:0 FAME作为主要脂肪酸 (>10%),二磷脂酰甘油,磷脂酰肌醇甘露糖苷,一种未鉴定的磷脂,三种未鉴定的极性脂质和两种未鉴定的糖脂作为其极性脂质,肽聚糖中的二氨基甲酸 (meso-DAP) 作为二氨基酸。基因组DNA的G + C含量为35.9%。通过表型和生理特征的差异,16S rRNA基因和基于基因组的系统发育的独特系统发育谱系以及低基因组相关性指数,可以将该菌株与梭状芽胞杆菌中的其他定义家族区分开。基于这些区别特性,提出了SYSU GA17129T菌株代表新的木聚糖11月属的新物种。11月,在一个新的木聚糖科家族中。11月新分类单元的模式种为SYSU GA17129T (= KCTC 15754T = CGMCC 1.5282T)。该菌株的特征是梭状芽胞杆菌的梭状芽胞杆菌类。