We constructed a recombinant industrial Saccharomyces cerevisiae yeast strain OC2-AXYL2-ABGL2-Xyl2 by inserting two copies of the β-glucosidase (BGL) and β-xylosidase (XYL) genes, and a gene cassette for xylose assimilation in the genome of yeast strain OC-2HUT. Both BGL and XYL were expressed on the yeast cell surface with high enzyme activities. Using OC2-AXYL2-ABGL2-Xyl2, we performed ethanol fermentation from a mixture of powdered cellulose (KC-flock) and Birchwood xylan, with the additional supplementation of a 30-g/l Trichoderma reesei cellulase complex mixture. The ethanol yield (gram per gram of added cellulases) of the strain OC2-AXYL2-ABGL2-Xyl2 increased approximately 2.5-fold compared to that of strain OC2-Xyl2, which lacked β-glucosidase and β-xylosidase activities. Notably, the concentration of additional T. reesei cellulase was reduced from 30 to 24 g/l without affecting ethanol production. The BGL- and XYL-displaying industrial yeast of the strain OC2-AXYL2-ABGL2-Xyl2 represents a promising yeast for reducing cellulase consumption of ethanol fermentation from lignocellulosic biomass by compensating for the inherent weak BGL and XYL activities of T. reesei cellulase complexes.

译文

我们通过插入两个拷贝的 β-葡萄糖苷酶 (BGL) 和 β-木糖苷酶 (XYL) 基因,以及在OC-2HUT酵母菌株基因组中用于木糖同化的基因盒,构建了重组工业酿酒酵母酵母菌株OC2-AXYL2-ABGL2-Xyl2。BGL和XYL均在高酶活性的酵母细胞表面表达。使用OC2-AXYL2-ABGL2-Xyl2,我们从粉状纤维素 (KC-flock) 和桦木木聚糖的混合物中进行了乙醇发酵,并补充了30g/l里氏木霉纤维素酶复合混合物。与缺乏 β-葡萄糖苷酶和 β-木糖苷酶活性的OC2-Xyl2菌株相比,该菌株OC2-AXYL2-ABGL2-Xyl2的乙醇产量 (克每克添加的纤维素酶) 增加了约2.5倍。值得注意的是,额外的里氏T纤维素酶的浓度从30g/l降低到24g/l,而不会影响乙醇的生产。OC2-AXYL2-ABGL2-Xyl2菌株的显示BGL和XYL的工业酵母代表了一种有前途的酵母,用于通过补偿里氏T纤维素酶复合物固有的弱BGL和XYL活性来减少木质纤维素生物质中乙醇发酵的纤维素酶消耗。

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