BACKGROUND & AIMS:
:This study determined the selenium (Se) bioavailability from Se-enriched garlic and cabbage using broiler chickens. Se-enriched garlic (18.5 mg of Se/kg) and cabbage (101.5 mg of Se/kg) were produced by soil enrichment using selenate. Conventional and Se-enriched garlic and cabbage were dried, ground, and added to broiler chick diets. Ninety-six broiler chickens at 1 day of age were assigned to four dietary treatments: NC (cabbage + garlic), PC (cabbage + garlic + selenomethionine, 0.5 mg of Se/kg of diet), GS (cabbage + Se-enriched garlic, 0.5 mg of Se/kg of diet), and CS (garlic + Se-enriched cabbage, 0.5 mg of Se/kg of diet), with six replicates per treatment and four birds per cage. Birds were fed the experimental diets for 4 weeks and slaughtered to obtain blood and tissues: white (breast) muscle, dark (thigh) muscle, liver, and feathers. All excreta were collected weekly, dried, and ground for Se analysis. Bird weight gain and feed intake were measured weekly. Total Se content and glutathione peroxidase (GPX) activity in liver and plasma were measured. Total liver Se content of the PC birds (0.876 mg of Se/kg) was the highest (P < .05). The CS (0.693 mg of Se/kg) and GS (0.627 mg of Se/kg) birds had higher (P < .05) total liver Se than the NC birds (0.514 mg of Se/kg). Plasma GPX activity of the PC birds was highest (P < .05), and that of CS and GS birds was higher (P < .05) than the NC birds. Liver GPX activity of the PC birds was higher (P < .05) than all other treatments. Bioavailability of Se to broiler chickens was not different (P > .05) among PC (65.2%), CS (61.2%), and GS (70.7%) birds. This study indicates that the Se from Se-enriched garlic and cabbage is highly bioavailable and can potentially be beneficial in enhancing Se status and GPX activity.
背景与目标:
:本研究使用肉鸡确定了富硒大蒜和卷心菜中硒的生物利用度。通过使用硒酸盐进行土壤富集,可以生产出富硒的大蒜(Se的含量为18.5 mg / kg)和卷心菜(Se的含量为101.5 mg / kg)。将常规的和富硒的大蒜和白菜干燥,磨碎,然后添加到肉鸡的日粮中。 1日龄的96只肉鸡被分配到四种饮食疗法中:NC(白菜大蒜),PC(白菜大蒜硒蛋氨酸,0.5 mg Se / kg饮食),GS(白菜中富含Se的大蒜,0.5 mg硒/千克日粮)和CS(大蒜富硒卷心菜,0.5毫克硒/千克日粮),每次处理重复六次,每笼饲养四只鸡。给鸟类喂食4周的实验饮食,并宰杀以获取血液和组织:白(乳房)肌肉,黑(大腿)肌肉,肝脏和羽毛。每周收集所有排泄物,干燥并研磨以进行Se分析。每周测量家禽体重增加和采食量。测量肝脏和血浆中的总硒含量和谷胱甘肽过氧化物酶(GPX)活性。 PC禽的总肝硒含量最高(Se / kg为0.876 mg)(P <0.05)。 CS(0.693 mg Se / kg)和GS(0.627 mg Se / kg)禽类的总肝Se含量高于NC禽类(0.514 mg Se / kg)(P <.05)。 PC鸟的血浆GPX活性最高(P <.05),CS和GS鸟的血浆GPX活性高于NC鸟(P <.05)。 PC禽的肝GPX活性高于所有其他处理(P <.05)。在PC(65.2%),CS(61.2%)和GS(70.7%)禽类中,硒对肉鸡的生物利用度没有差异(P> .05)。这项研究表明,富硒大蒜和卷心菜中的硒具有很高的生物利用度,并且可能在提高硒状态和GPX活性方面具有潜在的益处。