BACKGROUND & AIMS:
:Three bacteria namely Bacillus luciferensis K2, Bacillus amyloliquefaciens K12 and Bacillus subtilis BioCWB possessing plant growth promotion and biocontrol potential against phytopathogens and rice leaf folder were identified from organic soils of Sikkim, India. The results revealed significant higher production of phytohormones IAA (97.1 μg mL-1) and GA3 (10.6 μg mL-1) was found in K2, whereas BioCWB had higher phosphate solubilization (570.0 μg mL-1) efficacy and also possessed nitrogen fixation ability (5.34 log copy number mL-1 culture). All these bacteria had higher antagonistic activities against phytopathogens viz. Rhizoctonia solani, Fusarium proliferatum, Athelia rolfsii and Colletotrichum gloeosporioides and also had higher larvicidal activity against rice leaf folder Cnaphalocrocis medinalis (Guenne) under in vitro conditions. Molecular insights into the antagonistic mechanisms of Bacillus strains deciphered the presence of several antimicrobial peptides (ericin, subtilin, surfactin, iturin, bacilysin, subtilosin, fengycin and bacillomycin), volatiles (dimethyl disulphide, methyl-Furan, acetic acid, Z-1,3-pentadiene and 3-hexyn-2-ol) and soluble metabolites (9-octadecenamide, E-15-heptadecenal, E-3-eicosene and 5-octadecene). Furthermore, liquid microbial inoculum prepared using the bacterial strains (K2, K12 and BioCWB) were evaluated under glass house (rice) and field condition (capsicum), which significantly enhanced plant growth in rice and yield in capsicum compared to control. The present study revealed the combination of Bacillus spp. (K2, K12 and BioCWB) can be used as bio-inoculants for improving agricultural production in Sikkim. Moreover, for the first time, we demonstrated plant growth promoting (PGP) traits, antifungal and insecticidal properties of B. luciferensis.
背景与目标:
: 从印度锡金的有机土壤中鉴定出三种细菌,即萤光虫芽孢杆菌K2,解淀粉芽孢杆菌K12和枯草芽孢杆菌BioCWB,它们具有促进植物生长和对植物病原体和稻叶折叠的生物防治潜力。结果表明,在K2中发现了更高的植物激素IAA (97.1 μ g mL-1) 和GA3 (10.6 μ g mL-1),而BioCWB具有更高的磷酸盐增溶作用 (570.0 μ g mL-1),并且还具有固氮能力 (5.34对数拷贝数mL-1培养)。所有这些细菌对植物病原菌均具有较高的拮抗活性,即在体外条件下,对水稻叶夹棉,镰刀菌,镰刀菌和炭疽菌具有较高的杀幼虫活性。对芽孢杆菌菌株拮抗机制的分子见解揭示了几种抗菌肽 (ericin,subtilin,表面活性剂,伊图林,bacilysin,subtilosin,丰霉素和杆菌霉素),挥发物 (二甲基二硫化物,甲基呋喃,乙酸,Z-1,3-戊二烯和3-己-2-醇) 和可溶性代谢物 (9-十八烯酰胺,E-15-heptadecenal,e-3-eicosene和5-十八烯)。此外,在玻璃屋 (水稻) 和田间条件 (辣椒) 下评估了使用细菌菌株 (K2,K12和BioCWB) 制备的液体微生物接种物,与对照相比,这显着提高了水稻的植物生长和辣椒的产量。本研究揭示了芽孢杆菌属的组合。(K2,K12和BioCWB) 可用作生物接种剂,以改善锡金的农业生产。此外,我们首次证明了萤光虫的植物生长促进 (PGP) 性状,抗真菌和杀虫特性。