BACKGROUND & AIMS:
:Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet. Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa. The structure was elucidated based on monosaccharide composition and methylation analysis, NMR, FT-IR, and AFM analysis. It is mainly composed of mannose (46.6%), galactose (17.3%), arabinose (11.2%), glucose (10.5%), xylose (8.2%), fucose (5.2%), and rhamnose (1.0%). The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → . Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3. Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.
背景与目标:
:白酒的副产品hui水(HS)由于其营养成分和微生物成分而备受关注。然而,尚无研究探讨HS中的多糖。在这里,我们从HS分离出一种新型多糖HSP-3,其平均分子量为26.40 kDa。根据单糖组成和甲基化分析,NMR,FT-IR和AFM分析阐明了结构。它主要由甘露糖(46.6%),半乳糖(17.3%),阿拉伯糖(11.2%),葡萄糖(10.5%),木糖(8.2%),岩藻糖(5.2%)和鼠李糖(1.0%)组成。 HSP-3的骨架由→2)-β-d-Manp-(1→2,6)-β-d-Manp-(1→6)-β-d-Galp-(1→3组成,6)-β-d-Galp-(1→4)-α-l-Rhap-(1→3,4)-α-l-Rhap-(1→ ,TNF-α和IL-6,THP-1细胞中TNF-α和IL-6的mRNA和蛋白表达水平上调以及增强THP-1细胞的吞噬和吞噬能力表现出HSP-S的显着免疫调节特性。 3.总的来说,这项研究表明,HSP-3可以用作功能性食品中的活性成分。