BACKGROUND & AIMS: :Heterotetrameric sarcosine oxidase (TSOX) is a complex bifunctional flavoenzyme that contains two flavins. Most of the FMN in recombinant TSOX is present as a covalent adduct with an endogenous ligand. Enzyme denaturation disrupts the adduct, accompanied by release of a stoichiometric amount of sulfide. Enzyme containing>or=90% unmodified FMN is prepared by displacement of the endogenous ligand with sulfite, a less tightly bound competing ligand. Reaction of adduct-depleted TSOX with sodium sulfide produces a stable complex that resembles the endogenous TSOX adduct and known 4a-S-cysteinyl flavin adducts. The results provide definitive evidence for sulfide as the endogenous TSOX ligand and strongly suggest that the modified FMN is a 4a-sulfide adduct. A comparable reaction with sodium sulfide is not detected with other flavoprotein oxidases. A model of the postulated TSOX adduct suggests that it is stabilized by nearby residues that may be important in the electron transferase/oxidase function of the coenzyme.

背景与目标： : 异四聚肌氨酸氧化酶 (TSOX) 是一种复杂的双功能黄素酶，含有两种黄素。重组TSOX中的大多数FMN作为与内源性配体的共价加合物存在。酶变性破坏加合物，伴随着化学计量量的硫化物的释放。含有> 或 = 90% 未修饰的FMN的酶是通过用亚硫酸盐置换内源配体来制备的，亚硫酸盐是结合不太紧密的竞争配体。减少加合物的TSOX与硫化钠的反应产生稳定的络合物，类似于内源性TSOX加合物和已知的4a-s-半胱氨酸黄素加合物。结果为硫化物作为内源性TSOX配体提供了明确的证据，并强烈表明修饰的FMN是4a-硫化物加合物。与其他黄素蛋白氧化酶未检测到与硫化钠的类似反应。假定的TSOX加合物模型表明，它被附近的残基稳定，这可能对辅酶的电子转移酶/氧化酶功能很重要。

BACKGROUND & AIMS: :The oxidative deamination of serotonin (5-HT) to 5-hydroxyindoleacetic acid (5-HIAA) by rat primary astrocyte cultures was investigated in intact cells using HPLC. All detectable 5-HIAA accumulated in the extracellular medium, and its rate of production was proportional to the 5-HT concentration over the tested range of 5 x 10(-7) to 10(-4) M. At 5 x 10(-7) M 5-HT, intracellular 5-HT was detectable only in astrocytes treated with monoamine oxidase (MAO) inhibitors. These findings are consistent with the idea that 5-HT taken up into astrocytes is not stored for re-release, but is rapidly metabolized to 5-HIAA, which is then extruded from the cell. At 5 x 10(-7) M 5-HT, 5-HIAA formation in intact cells was blocked 63% by the selective high-affinity 5-HT uptake inhibitor fluoxetine. 5-HT oxidation to 5-HIAA is carried out principally by MAO-A, because clorgyline was more effective at inhibiting the production of 5-HIAA than was pargyline. Radioenzymatic determinations of MAO activity in cell homogenates supported these findings, because under these conditions clorgyline was 1,000-fold more effective than pargyline at inhibiting MAO activity toward 14C-labelled 5-HT. However, the relatively selective MAO-B substrate beta-phenylethylamine (PEA) was also oxidized, showing that these cultures also contained MAO-B activity; the Km values for MAO-A oxidation of 5-HT and MAO-B oxidation of PEA were 135 and 45 microM, and Vmax values were 88 and 91 nmol/mg of total cell protein/h, respectively. Higher concentrations of PEA (greater than 20 microM) were oxidized by both MAO-A and MAO-B isozymes.(ABSTRACT TRUNCATED AT 250 WORDS)

背景与目标： : 使用HPLC在完整细胞中研究了大鼠原代星形胶质细胞培养物将5-羟色胺 (5-HT) 氧化脱氨为5-羟基吲哚乙酸 (5-HIAA)。所有可检测到的5-HIAA都积累在细胞外培养基中，在5x10(-7) 至10(-4) M的测试范围内，其产生速率与5-HT浓度成正比。在5x10(-7) M 5-HT时，仅在用单胺氧化酶 (MAO) 抑制剂处理的星形胶质细胞中可检测到细胞内5-HT。这些发现与以下观点一致: 摄取到星形胶质细胞中的5-HT不会储存以重新释放，而是会迅速代谢为5-HIAA，然后从细胞中挤出。在5 × 10(-7) m5-ht时，选择性高亲和力5-HT摄取抑制剂氟西汀63% 阻断完整细胞中的5-HIAA形成。5-HT氧化为5-HIAA主要由MAO-A进行，因为clorgyline比pargyline更有效地抑制5-HIAA的产生。细胞匀浆中MAO活性的放射酶测定支持了这些发现，因为在这些条件下，clorgyline在抑制针对14c标记的5-HT的MAO活性方面比pargyline有效1,000倍。然而，相对选择性的MAO-B底物 β-苯乙胺 (PEA) 也被氧化，表明这些培养物也含有MAO-B活性; 5-HT的MAO-A氧化和PEA的MAO-B氧化的Km值分别为135和45微米，vmax值分别为88和91 nmol/mg的总细胞蛋白/h。较高浓度的豌豆 (大于20微米) 被MAO-A和MAO-B同工酶氧化。(摘要截短于250字)

BACKGROUND & AIMS: :Neutrophils cast neutrophil extracellular traps (NETs) to ensnare microbial pathogens. Nevertheless, the molecular rheostats that regulate NETosis in response to bacteria are not clearly established. We hypothesized that stress-activated protein kinase or c-Jun N-terminal Kinase (SAPK/JNK) is a molecular switch that turns on NETosis in response to increasing concentrations of lipopolysaccharide (LPS)- and Gram-negative bacteria. Here we show that Escherichia coli LPS (0111:B4; 10-25 μg/ml), but not phorbol myristate acetate (PMA), activates JNK in human neutrophils in a dose-dependent manner. JNK inhibitors SP600125 and TCSJNK6o, and a TLR4 inhibitor TAK242 suppress reactive oxygen species production and NETosis in LPS-, but not PMA-treated neutrophils. Diphenyleneiodonium suppresses LPS-induced NETosis, confirming that endotoxin induces NADPH oxidase-dependent NETosis. Immunoblots, Sytox Green assays, and confocal microscopy of cleaved caspase-3 and nuclear morphology show that JNK inhibition does not induce apoptosis in LPS-stimulated neutrophils. JNK inhibition also suppresses NETosis induced by two typical Gram-negative bacteria, E. coli and Pseudomonas aeruginosa. Therefore, we propose that neutrophils use a TLR4-dependent, JNK-mediated molecular sensing mechanism to initiate NADPH oxidase-dependent suicidal NETosis in response to increasing concentrations of LPS, and Gram-negative bacteria. The LPS-TLR4-JNK activation axis determines the fate of these cells: to be or not to be NETotic neutrophils.

背景与目标： 中性粒细胞投射中性粒细胞细胞外诱捕器 (NETs) 以诱捕微生物病原体。然而，尚未明确建立响应细菌调节NETosis的分子变阻剂。我们假设应激激活的蛋白激酶或c 6月的N端激酶 (SAPK/JNK) 是一种分子开关，可响应脂多糖 (LPS) 和革兰氏阴性菌浓度的增加而打开NETosis。在这里，我们显示大肠杆菌LPS (0111:B4; 10-25μg/ml)，而不是佛波醇肉豆蔻酸酯乙酸酯 (PMA)，以剂量依赖性方式激活人中性粒细胞中的JNK。JNK抑制剂SP600125和TCSJNK6o以及TLR4抑制剂TAK242抑制LPS-但不抑制PMA处理的嗜中性粒细胞中的活性氧产生和网状组织。二苯乙碘鎓抑制LPS诱导的NETosis，证实内毒素诱导NADPH氧化酶依赖性NETosis。免疫印迹，Sytox Green测定以及切割的caspase-3和核形态的共聚焦显微镜显示，JNK抑制不会诱导LPS刺激的中性粒细胞凋亡。JNK抑制也可以抑制由两种典型的革兰氏阴性菌 (大肠杆菌和铜绿假单胞菌) 诱导的网状病。因此，我们建议嗜中性粒细胞使用TLR4-dependent的JNK介导的分子传感机制来响应LPS和革兰氏阴性菌浓度的增加，启动NADPH氧化酶依赖性自杀网络。LPS-TLR4-JNK激活轴决定这些细胞的命运: 是或不是NETotic中性粒细胞。

BACKGROUND & AIMS: :The soya-derived phytoestrogen genistein has been suggested to be protective in cardiovascular diseases. In the present study, we have analysed whether chronic oral genistein might influence endothelial function in male SHRs (spontaneously hypertensive rats) via ERs (oestrogen receptors), changes in eNOS (endothelial NO synthase) activity and vascular O(2)(-) (superoxide) production. Rats (23-weeks old) were divided into the following groups: WKY (Wistar-Kyoto)-vehicle, SHR-vehicle, WKY-genistein (10 mg.kg(-1) of body weight.day(-1)); SHR-genistein; SHR-genistein-faslodex (ICI 182780; 2.5 mg.kg(-1) of body weight.day(-1)). Vascular expression of eNOS, caveolin-1 and calmodulin-1 were analysed by Western blotting, eNOS activity by conversion of [(3)H]arginine into L-[(3)H]citrulline and O(2)(-) production by chemoluminescence of lucigenin. In SHRs, after 5 weeks of treatment, genistein reduced systolic blood pressure and enhanced endothelium-dependent aortic relaxation to acetylcholine, but had no effect on the vasodilator responses to sodium nitroprusside. Compared with WKY rats, SHRs had up-regulated eNOS and down-regulated caveolin-1 and calmodulin-1 expression, increased NADPH-induced O(2)(-) production, but reduced eNOS activity. Genistein increased aortic calmodulin-1 protein abundance and eNOS activity, and reduced NADPH-induced O(2)(-) production in SHRs. The pure ERalpha and ERbeta antagonist faslodex did not modify any of the changes induced by genistein in SHRs, suggesting that these effects are unrelated to ER stimulation. In conclusion, genistein reduced the elevated blood pressure and endothelial dysfunction in SHRs. This latter effect appears to be related to increased eNOS activity associated with increased calmodulin-1 expression and decreased O(2)(-) generation.

背景与目标： : 大豆衍生的植物雌激素染料木黄酮被认为对心血管疾病具有保护作用。在本研究中，我们分析了慢性口服染料木黄酮是否可能通过ERs (雌激素受体)，eNOS (内皮NO合酶) 活性的变化和血管O(2)(-) 影响雄性shr (自发性高血压大鼠) 的内皮功能 (超氧化物) 产生。将大鼠 (23周龄) 分为以下组: WKY (Wistar-Kyoto)-载体，SHR-载体，WKY-染料木黄酮 (10 mg.kg(-1) 体重)。天 (-1)); SHR-染料木黄酮; SHR-genistein-faslodex (ICI 182780; 体重2.5 mg.kg(-1) 天 (-1))。通过蛋白质印迹法分析eNOS，caveolin-1和calmodulin-1的血管表达，通过将 [(3)H] 精氨酸转化为L-[(3)H] 瓜氨酸和通过荧光素的化学发光产生O(2)(-) 的eNOS活性。在shr中，治疗5周后，金雀异黄素降低了收缩压，增强了内皮依赖性主动脉对乙酰胆碱的舒张作用，但对硝普钠的血管舒张反应没有影响。与WKY大鼠相比，SHRs上调了eNOS，下调了caveolin-1和calmodulin-1表达，增加了NADPH诱导的O(2)(-) 产生，但降低了eNOS活性。染料木黄酮增加了主动脉calmodulin-1蛋白丰度和eNOS活性，并降低了NADPH诱导的shr中O(2)(-) 的产生。纯的ERalpha和ERbeta拮抗剂faslodex没有改变由染料木黄酮在shr中诱导的任何变化，提示这些作用与ER刺激无关。总之，染料木黄酮降低了shr的血压升高和内皮功能障碍。后一种作用似乎与与calmodulin-1表达增加和O(2)(-) 生成减少相关的eNOS活性增加有关。

BACKGROUND & AIMS: BACKGROUND:Atherosclerotic cardiovascular disease is the most common cause of death among hemodialysis patients; it has been attributed to increased oxidative stress, dyslipidemia, malnutrition, and chronic inflammation. Activation of neutrophils is a well-recognized feature in dialysis patients, and superoxide-anion production by neutrophil NADPH oxidase may contribute significantly to oxidative stress. OBJECTIVE:The aim of the study was to compare the effects of dietary supplementation with concentrated red grape juice (RGJ), a source of polyphenols, and vitamin E on neutrophil NADPH oxidase activity and other cardiovascular risk factors in hemodialysis patients. DESIGN:Thirty-two patients undergoing hemodialysis were recruited and randomly assigned to groups to receive dietary supplementation with RGJ, vitamin E, or both or a control condition without supplementation or placebo. Blood was obtained at baseline and on days 7 and 14 of treatment. RESULTS:RGJ consumption but not vitamin E consumption reduced plasma concentrations of total cholesterol and apolipoprotein B and increased those of HDL cholesterol. Both RGJ and vitamin E reduced plasma concentrations of oxidized LDL and ex vivo neutrophil NADPH oxidase activity. These effects were intensified when the supplements were used in combination; in that case, reductions in the inflammatory biomarkers intercellular adhesion molecule 1 and monocyte chemoattractant protein 1 also were observed. CONCLUSIONS:Regular ingestion of concentrated RGJ by hemodialysis patients reduces neutrophil NADPH-oxidase activity and plasma concentrations of oxidized LDL and inflammatory biomarkers to a greater extent than does that of vitamin E. This effect of RGJ consumption may favor a reduction in cardiovascular risk.

背景与目标：

BACKGROUND & AIMS: :Efferent innervation of the vestibular labyrinth is known to be cholinergic. More recent studies have also demonstrated the presence of the neuropeptide calcitonin gene-related peptide in this system. Nitric oxide is one of a new class of neurotransmitters, the gaseous transmitters. It acts as a second messenger and neurotransmitter in diverse physiological systems. We decided to investigate the anatomical distribution of the synthetic enzyme for nitric oxide, nitric oxide synthase (NOS), to clarify the role of nitric oxide in the vestibular periphery. NADPH diaphorase histochemical and NOS I immunohistochemical studies were done in the adult chinchilla and rat vestibular brainstem; diaphorase histochemistry was done in the chinchilla periphery. Retrograde tracing studies to verify the presence of NOS in brainstem efferent neurons were performed in young chinchillas. Our light microscopic results show that NOS I, as defined mainly by the presence of NADPH diaphorase, is present in a subpopulation of both brainstem efferent neurons and peripheral vestibular efferent boutons. Our ultrastructural results confirm these findings in the periphery. NADPH diaphorase is also present in a subpopulation of type I hair cells, suggesting that nitric oxide might be produced in and act locally upon these cells and other elements in the sensory epithelium. A hypothesis about how nitric oxide is produced in the vestibular periphery and how it may interact with other elements in the vestibular sensory apparatus is presented in the discussion.

背景与目标： 前庭迷路的传出神经支配已知是胆碱能的。最近的研究还证明了该系统中存在神经肽降钙素基因相关肽。一氧化氮是一类新的神经递质之一，即气态递质。它在各种生理系统中充当第二信使和神经递质。我们决定研究一氧化氮合成酶一氧化氮合酶 (NOS) 的解剖分布，以阐明一氧化氮在前庭周围的作用。在成年黄鼠和大鼠前庭脑干中进行了NADPH黄递酶组织化学和NOS I免疫组织化学研究; 黄递酶组织化学在黄鼠周围进行。在年轻的龙猫中进行了逆行追踪研究，以验证脑干传出神经元中NOS的存在。我们的光学显微镜结果表明，NOS I (主要由NADPH diapposase的存在定义) 存在于脑干传出神经元和外周前庭传出波顿的亚群中。我们的超微结构结果证实了外围的这些发现。NADPH diappoase也存在于I型毛细胞的亚群中，这表明一氧化氮可能在这些细胞和感觉上皮中的其他元素中产生并局部作用于这些细胞。讨论中提出了有关一氧化氮如何在前庭周围产生以及它如何与前庭感觉装置中的其他元素相互作用的假设。

BACKGROUND & AIMS: :Abscisic acid (ABA) is a plant hormone involved in seed development and germination and in responses to various environmental stresses. The last step of ABA biosynthesis involves oxidation of abscisic aldehyde, and aldehyde oxidase (EC ) is thought to catalyze this reaction. An aldehyde oxidase isoform, AOdelta, encoded by AAO3, one of four Arabidopsis aldehyde oxidase genes (AAO1, AAO2, AAO3, and AAO4), is the most likely candidate for the enzyme, because it can efficiently catalyze the oxidation of abscisic aldehyde to ABA. Here, we report the isolation and characterization of an ABA-deficient Arabidopsis mutant that maps at the AAO3 locus. The mutant exhibits a wilty phenotype in rosette leaves, but seed dormancy is not affected. ABA levels were significantly reduced in the mutant leaves, explaining the wilty phenotype in rosettes, whereas the level in the mutant seeds was less reduced. No AOdelta activity could be detected in the rosette leaves of the mutant. Sequence data showed that the mutant contains a G to A substitution in the AAO3 gene. The mutation causes incorrect splicing of the ninth intron of AAO3 mRNA. We thus conclude that the ABA-deficient mutant is impaired in the AAO3 gene and that the gene product, AOdelta, is an aldehyde oxidase that catalyzes the last step of ABA biosynthesis in Arabidopsis, specifically in rosette leaves. Other aldehyde oxidases may be involved in ABA biosynthesis in other organs.

背景与目标： 脱落酸 (ABA) 是一种植物激素，参与种子发育和发芽以及对各种环境胁迫的响应。ABA生物合成的最后一步涉及脱落酸醛的氧化，醛氧化酶 (EC) 被认为可以催化该反应。由四个拟南芥醛氧化酶基因 (AAO1，AAO2，AAO3和AAO4) 之一的AAO3编码的醛氧化酶同工型AOdelta是该酶的最可能候选者，因为它可以有效地催化脱落酸醛向ABA的氧化。在这里，我们报告了定位在AAO3基因座的ABA缺陷型拟南芥突变体隔离和表征。该突变体在玫瑰花结叶片中表现出枯萎表型，但种子休眠不受影响。突变叶片中的ABA水平显着降低，这解释了玫瑰花结中的wilty表型，而突变种子中的水平降低较少。在突变体的莲座丛叶中未检测到AOdelta活性。序列数据表明，该突变体在AAO3基因中含有一个G到a的取代。该突变导致AAO3 mRNA第九个内含子的不正确剪接。因此，我们得出结论，ABA缺陷型突变体在AAO3基因中受损，并且基因产物AOdelta是一种醛氧化酶，可催化拟南芥 (特别是莲座叶) 中ABA生物合成的最后一步。其他醛氧化酶可能参与其他器官的ABA生物合成。

BACKGROUND & AIMS: :D-Amino acid oxidase (DAAO), a FAD-dependent peroxisomal flavoenzyme that catalyzes oxidation of D-amino acids to hydrogen peroxide, is distributed in the spinal cord almost exclusively expressed within astrocytes. The present study aims to explore potential contributions of spinal DAAO to the development of bone cancer pain and morphine tolerance to analgesia. Tibia inoculation of carcinoma cells produced mechanical allodynia (but not heat hyperalgesia), in synchronous with induction of DAAO expression and DAAO enzymatic activity, as well as activation of spinal astrocytes marked by GFAP. Subcutaneous and intrathecal injection of the specific DAAO inhibitor CBIO (5-chloro-benzo[d]isoxazol-3-ol) blocked mechanical allodynia in a dose- and time-dependent manner in tumor-bearing rats, with maximum inhibition of 40-50 %. Multi-daily intrathecal injections of the DAAO gene silencer siRNA/DAAO also yielded anti-allodynic effects by approximately 40 % and the analgesia remained for at least 6 days. Subcutaneous injection of CBIO suppressed the production of spinal hydrogen peroxide and GFAP expression. 7-Day multiple bi-daily injections of CBIO produced anti-allodynia without inducing self-tolerance to analgesia or cross-tolerance to morphine, and concurrent injections of CBIO with morphine produced apparent additive anti-allodynia and completely prevented morphine tolerance in behaviors and spinal expression of μ-opioid receptors. Our results provide the first evidence that spinal DAAO contributes to the development of morphine tolerance to analgesia and bone cancer pain accounting for 40-50 % pain status, probably via production of hydrogen peroxide leading to activation of astrocytes. The unique characterizations of DAAO inhibitors make them a potential for the treatment of cancer pain when they are administered alone or in combination with morphine.

背景与目标： : D-氨基酸氧化酶 (DAAO) 是一种FAD依赖性过氧化物酶体黄素酶，可催化D-氨基酸氧化为过氧化氢，分布在脊髓中，几乎仅在星形胶质细胞内表达。本研究旨在探讨脊髓DAAO对骨癌疼痛和吗啡镇痛耐受性的潜在贡献。与DAAO表达和DAAO酶活性的诱导以及GFAP标记的脊髓星形胶质细胞的激活同步，胫骨接种癌细胞会产生机械性异常性疼痛 (但不会产生热痛觉过敏)。皮下和鞘内注射特定的DAAO抑制剂CBIO (5-氯-苯并 [d]isoxazol-3-ol) 以剂量和时间依赖性方式阻断荷瘤大鼠的机械性异常性疼痛，最大抑制作用为40-50%。DAAO基因沉默子siRNA/DAAO的多天鞘内注射也产生了约40% 的抗异常性作用，镇痛作用保持至少6天。皮下注射CBIO抑制了脊髓过氧化氢的产生和GFAP的表达。7天多次双日注射CBIO可产生抗异常性疼痛，而不会引起对镇痛的自我耐受性或对吗啡的交叉耐受性，同时注射CBIO与吗啡可产生明显的累加性抗异常性疼痛，并完全阻止行为和行为中的吗啡耐受性。μ 阿片受体的脊髓表达。我们的结果提供了第一个证据，表明脊髓DAAO有助于吗啡对镇痛和骨癌疼痛的耐受性的发展，这可能是通过产生过氧化氢导致星形胶质细胞活化而导致的40-50% 疼痛状态。DAAO抑制剂的独特特征使其成为单独或与吗啡联合使用时治疗癌症疼痛的潜力。

BACKGROUND & AIMS: :Although hyperuricemia has been shown to be associated with the progression of cardiovascular disorder and chronic kidney disease (CKD), there is conflicting evidence as to whether xanthine oxidase (XO) inhibitors confer organ protection besides lowering serum urate levels. In this study, we addressed the cardio-renal effects of XO inhibition in rodent CKD model with hyperuricemia. Sprague-Dawley rats underwent 5/6 nephrectomy and received a uricase inhibitor oxonic acid for 8 weeks (RK + HUA rats). In some rats, a XO inhibitor febuxostat was administered orally. Compared with control group, RK + HUA group showed a significant increase in albuminuria and renal injury. Febuxostat reduced serum uric acid as well as urinary albumin levels. Histological and immunohistochemical analysis of the kidney revealed that febuxostat alleviated glomerular, tubulointerstitial, and arteriolar injury in RK + HUA rats. Moreover, in the heart, RK + HUA showed individual myofiber hypertrophy and cardiac fibrosis, which was significantly attenuated by febuxostat. We found that renal injury and the indices of cardiac changes were well correlated, confirming the cardio-renal interaction in this model. Finally, NF-E2-related factor 2 (Nrf2) and the downstream target heme oxygenase-1 (HO-1) protein levels were increased both in the heart and in the kidney in RK + HUA rats, and these changes were alleviated by febuxostat, suggesting that tissue oxidative stress burden was attenuated by the treatment. These data demonstrate that febuxostat protects against cardiac and renal injury in RK + HUA rats, and underscore the pathological importance of XO in the cardio-renal interaction.

背景与目标： : 尽管高尿酸血症已被证明与心血管疾病和慢性肾脏疾病 (CKD) 的进展有关，但关于黄嘌呤氧化酶 (XO) 抑制剂是否除了降低血清尿酸水平外还具有器官保护作用，存在相互矛盾的证据。在这项研究中，我们讨论了XO抑制在患有高尿酸血症的啮齿动物CKD模型中的心肺作用。Sprague-Dawley大鼠接受5/6肾切除术，并接受尿酸酶抑制剂oxonic酸治疗8周 (rk   +  HUA大鼠)。在某些大鼠中，口服XO抑制剂非布索坦。与对照组相比，rk   +  HUA组蛋白尿和肾损伤明显增加。非布索坦可降低血清尿酸和尿白蛋白水平。肾脏的组织学和免疫组织化学分析表明，非布司他减轻了rk   hua hua大鼠的肾小球，肾小管间质和小动脉损伤。此外，在心脏中，rk   +  HUA表现为单个肌纤维肥大和心脏纤维化，非布沙坦明显减弱。我们发现肾损伤与心脏变化的指标具有良好的相关性，从而证实了该模型中的心-肾相互作用。最终，NF-E2-related因子2 (Nrf2) 和下游靶血红素oxygenase-1 (HO-1) 蛋白在rk   +  HUA大鼠心脏和肾脏中的水平均升高，非布沙坦缓解了这些变化，提示该治疗减轻了组织氧化应激负荷。这些数据表明，非布索坦可预防rk   hua hua大鼠的心脏和肾脏损伤，并强调了XO在心肺相互作用中的病理重要性。

BACKGROUND & AIMS: :We studied whether angiotensin II (ANG II) via superoxide may contribute to retinal leukostasis and thus to the pathogenesis of retinopathies. We studied: 1) whether intravitreal ANG II induces retinal leukostasis that is altered by antioxidants or by apocynin, a NAD(P)H oxidase inhibitor and 2) whether retinal leukostasis induced by diabetes in rats is also altered by these treatments. Rats were injected intravitreally with ANG II (20 microg in 2 microl), and divided into the following three groups: 1) untreated; 2) treated with tempol doses ( approximately 3 mM/day) and N-acetylcysteine (NAC; approximately 1 g.kg(-1).day(-1)); and 3) treated with apocynin ( approximately 2 mM/day), both in the drinking water. Rats with streptozotocin-induced diabetes were similarly treated. Leukostasis was evaluated 48 h after ANG II or 2 wk after diabetes induction. ANG II increased retinal leukostasis from 0.3 +/- 0.5 to 3.7 +/- 0.4 leukocytes/ mm(2) (P < 0.01), and these changes were markedly decreased by treatment with tempol + NAC or apocynin, and also by a blocking antibody against vascular endothelial growth factor given intravitreally (P < 0.01). In addition, incubation of dihydroethidium-loaded retina sections with ANG II caused marked increase in superoxide formation. Compared with normal controls, retinal leukostasis in diabetic rats markedly increased from 0.2 +/- 0.3 to 3.8 +/- 0.1 leukocytes/mm(2) (P < 0.01). Diabetic retinal leukostasis was also decreased by treatment with tempol-NAC and normalized by apocynin. Thus increases in intravitreal ANG II can induce retinal leukostasis, which appears to be mediated via increasing superoxide generation by NAD(P)H oxidase, and by VEGF. The activity of NAD(P)H oxidase is required for leukostasis to occur in the diabetic retina.

背景与目标： : 我们研究了通过超氧化物引起的血管紧张素II (ANG II) 是否可能导致视网膜白细胞平衡，从而导致视网膜病变的发病机理。我们研究了: 1) 玻璃体内ANG II是否诱导了由抗氧化剂或apocynin (一种NAD(P)H氧化酶抑制剂) 改变的视网膜白细胞平衡，以及2) 这些治疗是否也改变了糖尿病引起的大鼠视网膜白细胞平衡。大鼠玻璃体内注射ANG II (2 microl中的20 microg)，并分为以下三组: 1) 未经治疗; 2) 用tempol剂量 (约3毫米/天) 和N-乙酰半胱氨酸 (NAC) 处理; 约1g.kg(-1) 天 (-1)); 和3) 用apocynin (约2毫米/天) 处理，两者均在饮用水中。对链脲佐菌素诱导的糖尿病大鼠进行了类似的治疗。在ANG II后48小时或糖尿病诱导后2周评估白细胞平衡。ANG II使视网膜白细胞从0.3 +/- 0.5增加到3.7 +/- 0.4白细胞/mm(2) (P <0.01)，这些变化通过tempol + NAC或apocynin治疗明显减少，并且还通过抗血管内皮生长因子的阻断抗体给予玻璃体内注射 (P <0.01)。此外，将载有二氢乙锭的视网膜切片与ANG II孵育会导致超氧化物形成显着增加。与正常对照组相比，糖尿病大鼠的视网膜白细胞平衡从0.2/0.3显着增加到3.8/0.1/mm(2) (P <0.01)。用tempol-NAC治疗也可以减少糖尿病性视网膜白细胞平衡，并通过apocynin恢复正常。因此，玻璃体内ANG II的增加可以诱导视网膜白细胞平衡，这似乎是通过NAD(P)H氧化酶和VEGF增加超氧化物生成来介导的。NAD(P)H氧化酶的活性是糖尿病视网膜中发生白细胞平衡所必需的。

BACKGROUND & AIMS: :Until the 1970s, reactive oxygen species (ROS) were considered merely harmful by-products of aerobic respiration and the driving force behind the evolution of an array of cellular antioxidant enzymes with the purpose of rapidly metabolising ROS to minimise their oxidising effects. However, the perception that ROS are only harmful to cells has since been questioned by a burgeoning body of evidence pointing to the existence of enzymes with the dedicated function of generating ROS. NADPH oxidases represent the only known family of enzymes whose sole purpose is to generate ROS. Members of this enzyme family are expressed across mammalian and non-mammalian cells, and influence a multitude of biological functions including host defence and redox signalling. However, although ROS are deliberately generated by NADPH oxidases during normal cell physiology, the observations that their expression and activity is markedly upregulated in the blood vessel wall in a number of cardiovascular 'high-risk' states (e.g. hypertension, hypercholesterolemia) implicates them in the oxidative stress that gives rise to artery disease and ultimately heart attacks and strokes. These observations highlight the fact that NADPH oxidases are important therapeutic targets in cardiovascular disease and that, hence, there is clearly a need for the development of selective inhibitors of these enzymes. Here we highlight the structural and biochemical characteristics of the NADPH oxidase family and then comprehensively review the literature on the currently available pharmacological inhibitors of these enzymes with a particular emphasis on their mechanisms of action, isoform selectivity and therapeutic potential in cardiovascular disease.

背景与目标： : 在20世纪70年代之前，活性氧 (ROS) 仅被认为是有氧呼吸的有害副产物，也是一系列细胞抗氧化酶进化的驱动力，目的是快速代谢ROS以最大程度地减少其氧化作用。然而，自那以后，越来越多的证据表明存在具有生成ROS的专用功能的酶，从而质疑ROS仅对细胞有害的看法。NADPH氧化酶代表唯一已知的酶家族，其唯一目的是产生ROS。该酶家族的成员在哺乳动物和非哺乳动物细胞中表达，并影响多种生物学功能，包括宿主防御和氧化还原信号传导。然而，尽管ROS是在正常细胞生理过程中由NADPH氧化酶故意产生的，但在许多心血管 “高风险” 状态 (例如高血压，高胆固醇血症) 使它们参与氧化应激，从而导致动脉疾病并最终导致心脏病发作和中风。这些观察结果强调了一个事实，即NADPH氧化酶是心血管疾病的重要治疗靶标，因此，显然需要开发这些酶的选择性抑制剂。在这里，我们重点介绍了NADPH氧化酶家族的结构和生化特征，然后全面回顾了有关这些酶的当前可用药理抑制剂的文献，特别强调了它们的作用机制，同工型选择性和在心血管疾病中的治疗潜力。

BACKGROUND & AIMS: :In cytochrome c oxidase, oxido-reductions of heme a/Cu(A) and heme a3/Cu(B) are cooperatively linked to proton transfer at acid/base groups in the enzyme. H+/e- cooperative linkage at Fe(a3)/Cu(B) is envisaged to be involved in proton pump mechanisms confined to the binuclear center. Models have also been proposed which involve a role in proton pumping of cooperative H+/e- linkage at heme a (and Cu(A)). Observations will be presented on: (i) proton consumption in the reduction of molecular oxygen to H2O in soluble bovine heart cytochrome c oxidase; (ii) proton release/uptake associated with anaerobic oxidation/reduction of heme a/Cu(A) and heme a3/Cu(B) in the soluble oxidase; (iii) H+ release in the external phase (i.e. H+ pumping) associated with the oxidative (R-->O transition), reductive (O-->R transition) and a full catalytic cycle (R-->O-->R transition) of membrane-reconstituted cytochrome c oxidase. A model is presented in which cooperative H+/e- linkage at heme a/Cu(A) and heme a3/Cu(B) with acid/base clusters, C1 and C2 respectively, and protonmotive steps of the reduction of O2 to water are involved in proton pumping.

背景与目标： : 在细胞色素c氧化酶中，血红素a/Cu(A) 和血红素a3/Cu(B) 的氧化还原与酶中酸/碱基团的质子转移协同连接。预计Fe(a3)/Cu(B) 处的H/e协同连接涉及限于双核中心的质子泵机制。还提出了一些模型，这些模型涉及在血红素a (和Cu (a)) 的协同H/e键的质子泵送中的作用。将提出以下观察结果 :( i) 将可溶性牛心脏细胞色素c氧化酶中的分子氧还原为H2O的质子消耗; (ii) 与可溶性氧化酶中血红素a/Cu(A) 和血红素a3/Cu(B) 的厌氧氧化/还原相关的质子释放/吸收; (iii) 与氧化 (R->O转变) 相关的外部相 (即H泵) 中的H释放，膜重建的细胞色素c氧化酶的还原 (O->R跃迁) 和全催化循环 (R->O->R跃迁)。提出了一个模型，其中血红素A/Cu (a) 和血红素a3/Cu(B) 分别具有酸/碱簇C1和C2的协同H/e键，以及将O2还原为水的质子运动步骤参与质子泵送。

BACKGROUND & AIMS: BACKGROUND:Nitric oxide (NO) is an important signal molecule in many types of cells and tissues. Efficiently balanced NO production was noted to play an important role in the healing of burns. However, the exact pathophysiological role of NO in burns and its potent relation with clinical and laboratory parameters has not been elucidated. METHODS:A cohort of 23 burn patients followed for 5 days were enrolled. NO, antioxidant capacity (AC), NO synthase (NOS) activity and xanthine oxidase (XO) activity were indirectly determined by fluorophotometer. Multiple regression against total burn surface area (TBSA), age, weight, height, proximity of septic episode, hemoglobin, white blood cells, percent of neutrophils, platelets, glucose, urea, potassium, sodium and albumin was performed. RESULTS:Elevation of NO, XO and AC levels is observed from day 2 (p<0.00001), day 4 (p=0.005) and day 6 (p=0.036), respectively. At the end of follow-up period (day 6), NO production was found to independently correlate with TBSA, glucose levels and percent of neutrophils (p=0.0004), AC with age, hemoglobin and glucose levels (p=0.012), and NOS with proximity of septic episode and glucose levels (p=0.027). CONCLUSIONS:NO production exerts its prophylactic effect from the first 24h after burn, and is independently correlated with severe injury, enhanced neutrophil motivation and augmented glucose levels, thus possibly representing a response to stress. This need might trigger induction of XO and salvage of antioxidants, as suggested by their rise at a later stage. These data underline that an effort to compromise stress and to administer antioxidants could be a priority in the treatment of these patients.

背景与目标：

BACKGROUND & AIMS: :The production of reactive oxygen species (ROS) by neutrophils has a vital role in defence against a range of infectious agents, and is driven by the assembly of a multi-protein complex containing a minimal core of five proteins: the two membrane-bound subunits of cytochrome b(558) (gp91(phox) and p22(phox)) and three soluble factors (GTP-Rac, p47(phox) and p67(phox) (refs 1, 2). This minimal complex can reconstitute ROS formation in vitro in the presence of non-physiological amphiphiles such as SDS. p40(phox) has subsequently been discovered as a binding partner for p67(phox) (ref. 3), but its role in ROS formation is unclear. Phosphoinositide-3-OH kinases (PI(3)Ks) have been implicated in the intracellular signalling pathways coordinating ROS formation but through an unknown mechanism. We show that the addition of p40(phox) to the minimal core complex allows a lipid product of PI(3)Ks, phosphatidylinositol 3-phosphate (PtdIns(3)P), to stimulate specifically the formation of ROS. This effect was mediated by binding of PtdIns(3)P to the PX domain of p40(phox). These results offer new insights into the roles for PI(3)Ks and p40(phox) in ROS formation and define a cellular ligand for the orphan PX domain.

背景与目标： : 嗜中性粒细胞产生活性氧 (ROS) 在防御一系列感染因子方面具有至关重要的作用，并且是由包含五种蛋白质的最小核心的多蛋白复合物的组装驱动的: 细胞色素b的两个膜结合亚基 (558) (gp91(phox) 和p22(phox)) 和三个可溶性因子 (gtp-rac，p47(phox) 和p67(phox) (参考文献1，2)。这种最小复合物可以在非生理两亲物如SDS的存在下在体外重建ROS的形成。p40(phox) 随后被发现作为p67(phox) 的结合伴侣 (参考文献3)，但是它在ROS形成中的作用尚不清楚。Phosphoinositide-3-OH激酶 (PI(3)Ks) 已与协调ROS形成的细胞内信号通路有关，但通过未知的机制。我们表明，将p40(phox) 添加到最小核心复合物中可以使PI(3)Ks的脂质产物,磷脂酰肌醇3-磷酸 (PtdIns(3)P)，具体地刺激ROS的形成。这种作用是通过PtdIns(3)P与p40(phox) 的PX结构域的结合来介导的。这些结果为PI(3)Ks和p40(phox) 在ROS形成中的作用提供了新的见解，并为孤儿定义了细胞配体PX域。

BACKGROUND & AIMS: :The anthracycline anticancer agents daunorubicin (DAUN) and doxorubicin (DOX) are reduced by different NADPH-dependent cytosolic reductases into their corresponding alcohol metabolites daunorubicinol (DAUNol) and doxorubicinol (DOXol), which have been implicated in the development of chronic cardiomyopathy. To better understand the individual importance of each enzyme in the reduction and to provide deeper insight into the binding at atomic level we performed molecular docking and dynamics simulations of DAUN and DOX into the active sites of human carbonyl reductase 1 (CBR1) and human aldehyde reductase (AKR1A1). Such simulations evidenced a different behavior between the reductases with respect to DAUN and DOX suggesting major contribution of CBR1 in the reduction. The results are in agreement with available experimental data and for each enzyme and anthracycline pair provided the identification of key residues involved in the interactions. The structural models that we have derived could serve as a useful tool for structure-guided drug design studies.

背景与目标： : 蒽环类抗癌剂柔红霉素 (DAUN) 和阿霉素 (DOX) 被不同的NADPH依赖性胞质还原酶还原成其相应的醇代谢产物柔红霉素 (DAUNol) 和阿霉素 (DOXol)，这与慢性心肌病的发展有关。为了更好地了解每种酶在还原中的个体重要性并提供对原子水平结合的更深入的了解，我们进行了DAUN和DOX到人羰基还原酶1 (CBR1) 和人的活性位点的分子对接和动力学模拟醛还原酶 (AKR1A1)。这样的模拟证明了还原酶之间相对于DAUN和DOX的不同行为，表明CBR1在还原中的主要贡献。结果与可用的实验数据一致，并且对于每种酶和蒽环类药物对，都提供了相互作用中涉及的关键残基的鉴定。我们得出的结构模型可以作为结构指导药物设计研究的有用工具。