EMX2 and PAX6 are expressed by cortical progenitors and specify area patterning. We used representational difference analysis (RDA) to compare expressed RNAs from wild type and Emx2-/- cortex and identified 41 unique clones. Using secondary screening by in situ hybridization, we selected five genes for further analysis, Cdk4, Cofilin1, Crmp1, ME2, and Odz4, involved in neuronal proliferation, differentiation, migration, and axon guidance. Each exhibits differential expression in wild type cortex. Odz4 is one of four members of a vertebrate gene family homologous to the Drosophila pair-rule patterning gene, Odd Oz (Odz), a transmembrane receptor. We show that Odz genes are expressed in complementary patterns in cortex, as well as in nuclei-specific patterns in thalamus that relates to their area-unique cortical expression. In addition, each of the genes analyzed shows different expression patterns in wild type cortex, Emx2, and Pax6 mutant cortex, consistent with potential roles in area patterning. These findings identify potential targets of EMX2 that might account for its function and the defects in Emx2-/- cortex, and suggest that the Odz family of transmembrane proteins influences cortical area patterning downstream to EMX2 and PAX6.

译文

:EMX2和PAX6由皮质祖细胞表达,并指定区域构图。我们使用代表性差异分析(RDA)来比较野生型和Emx2-/-皮质表达的RNA,并鉴定出41个独特的克隆。使用通过原位杂交的二次筛选,我们选择了五个基因进行进一步分析,分别是Cdk4,Cofilin1,Crmp1,ME2和Odz4,它们参与神经元增殖,分化,迁移和轴突引导。每种在野生型皮质中表现出差异表达。 Odz4是与果蝇对规则模式基因跨膜受体Odd Oz(Odz)同源的脊椎动物基因家族的四个成员之一。我们显示,Odz基因在皮质中的互补模式中表达,以及在丘脑中与它们的面积独特的皮质表达有关的特定于细胞核的模式中表达。此外,分析的每个基因在野生型皮层,Emx2和Pax6突变皮层中均显示出不同的表达模式,这与区域模式中的潜在作用一致。这些发现确定了可能解释其功能和Emx2-/-皮质缺陷的EMX2潜在靶标,并表明跨膜蛋白的Odz家族影响了EMX2和PAX6下游的皮质区域构图。

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