Four strains of ascomycetous yeasts were isolated from samples collected at two locations in southern Japan. The strains formed two warty ascospores that were joined together by an intersporal body appearing as a belt. Phylogenetic analysis of rRNA gene nucleotide sequences indicated that the strains represented two new and closely related species of the genus Kazachstania. Isolates of one of the species were from Miyazaki Prefecture and those of the other species were from the Iriomote Islands. Genetic separation of the two species was further confirmed by DNA-DNA reassociation, which gave values of 63.3-78.1%, and from interspecific crosses, which gave nonviable ascospores. On the basis of these data, the isolates from Miyazaki Prefecture are described as Kazachstania zonata sp. nov. [type strain NBRC 100504=CBS 10326, mating types NBRC 101821 (+), NBRC 101822 (-)], and the isolates from the Iriomote Islands are described as Kazachstania gamospora sp. nov. [type strain NBRC 11056=CBS 10328, mating types NBRC 101825 (+), NBRC 101826 (-)].

译文

:从日本南部两个地方收集的样品中分离出四株子囊酵母。这些菌株形成了两个疣状的子囊孢子,它们通过一个呈带状的孢子间体连接在一起。 rRNA基因核苷酸序列的系统发育分析表明,该菌株代表了哈萨克斯坦属的两个新的且密切相关的物种。其中一个物种的分离物来自宫崎县,而其他物种的分离物则来自西表岛。这两个物种的遗传分离进一步通过DNA-DNA重新关联(其值为63.3-78.1%)以及种间杂交获得了无生命的子囊孢子而得到证实。基于这些数据,来自宫崎县的分离株被描述为Kazachstania zonata sp.。十一月[类型菌株NBRC 100504 = CBS 10326,交配类型NBRC 101821(),NBRC 101822(-)]和来自西表岛的分离物被描述为Kazachstania gamospora sp.。十一月[类型菌株NBRC 11056 = CBS 10328,配合类型NBRC 101825(),NBRC 101826(-)]。

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