OBJECTIVE:To observe the effects of anti-fecundity and anti-embryonation immunity of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum on female adult worm.
METHODS:The active immunization of C57BL/6 mice was conducted by means of three intraperitoneal injections of NP30. The control group was injected with SP2/0 ascites intraperitoneally.
RESULTS:On the twenty-seventh day after challenge infection, the number of eggs in the liver tissue and in uterus of the group immunized with NP30 decreased by 30.91% and by 38.55%, respectively. On the thirty-ninth day after the challenge infection, the number of mature eggs in the liver tissue of the group immunized with NP30 decreased by 66.63% and the number of dead eggs increased by 60.66%.
CONCLUSIONS:NP30, with which mice were actively immunized, possesses double effects of anti-fecundity and anti-embryonation immunity on female adult worm of Schistosoma japonicum, therefore it can be used as a promising candidate of anti-pathologic vaccine molecule against Schistosomiasis japonica.
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【Anti-fecundity immunity in mice immunized with anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum.].
【用日本血吸虫的抗独特型单克隆抗体NP30免疫的小鼠的抗生殖力免疫力。】
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目的：观察日本血吸虫抗独特型单克隆抗体NP30对成虫的抗生殖力和抗胚芽免疫的作用。
方法：通过腹膜内注射3次NP30对C57BL / 6小鼠进行主动免疫。对照组腹腔注射SP2 / 0腹水。
结果：在攻击感染后的第二十七天，NP30免疫组的肝脏组织和子宫中的卵数分别减少了30.91％和38.55％。攻击感染后的第39天，NP30免疫组的肝脏组织中成熟卵的数量减少了66.63％，死卵的数量增加了60.66％。
结论：主动免疫小鼠的NP30对日本血吸虫成虫具有抗生殖力和抗胚芽免疫的双重作用，因此可作为抗日本血吸虫病的病理疫苗分子的有前途的候选者。
方法：通过腹膜内注射3次NP30对C57BL / 6小鼠进行主动免疫。对照组腹腔注射SP2 / 0腹水。
结果：在攻击感染后的第二十七天，NP30免疫组的肝脏组织和子宫中的卵数分别减少了30.91％和38.55％。攻击感染后的第39天，NP30免疫组的肝脏组织中成熟卵的数量减少了66.63％，死卵的数量增加了60.66％。
结论：主动免疫小鼠的NP30对日本血吸虫成虫具有抗生殖力和抗胚芽免疫的双重作用，因此可作为抗日本血吸虫病的病理疫苗分子的有前途的候选者。
