A study was conducted to determine the changes that occur to proteolysis and related genes due to age, protein, and energy intake in high-yield broiler breeder hens (Gallus gallus). Cobb 700 broiler breeders were randomly assigned to one of six diets in a 2×3 factorial fashion. Two levels of energy (390 and 450 kcal/day) and three levels of protein (22, 24, and 26 g CP/day) were utilized. Protein turnover was determined in the left pectoralis at 22, 26, 31 and 44 weeks. Relative mRNA expression of calpain 2 (CAPN2), proteasome C2 subunit (PSMA1), and F box protein 32 (FBXO32) were determined via RT-PCR at 20, 25, and 44 weeks. Contrasts indicate fractional synthesis rate (FSR) and FBXO32 increase to a maximum at 25-26 weeks and a decrease thereafter. A significant drop in PSMA1 and FBXO32 was observed between 25 and 44 weeks and matched the decrease observed in FBR. No differences were detected in the levels of fractional synthesis and degradation, or the expression of CAPN2, PSMA1, and FBXO32, due to protein or energy intake. In summary, protein turnover was upregulated during the transition into sexual maturity and decreased thereafter. The observed changes in degradation appeared to be mediated by the ubiquitin-proteasome pathway.

译文

进行了一项研究,以确定由于年龄,蛋白质和能量摄入而导致的蛋白水解和相关基因发生的变化。高产肉鸡种鸡 (Gallus gallus)。将Cobb 700肉鸡饲养者以2 × 3因子的方式随机分配到六种饮食中的一种。利用两个水平的能量 (390和450 kcal/天) 和三个水平的蛋白质 (22、24和26g CP/天)。在22、26、31和44周时确定左胸肌的蛋白质更新。在第20、25和44周通过rt-pcr测定calpain 2 (CAPN2),蛋白酶体C2亚基 (PSMA1) 和F box蛋白32 (FBXO32) 的相对mRNA表达。对比表明分数合成速率 (FSR) 和FBXO32在25-26周时增加到最大值,此后降低。在25到44周之间观察到PSMA1和FBXO32的显着下降,并且与FBR的下降相匹配。由于蛋白质或能量摄入,在分数合成和降解水平或CAPN2,PSMA1和FBXO32的表达中未检测到差异。总而言之,在过渡到性成熟期间,蛋白质更新上调,此后降低。观察到的降解变化似乎是由泛素-蛋白酶体途径介导的。

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