Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor) using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l(-1) and 50 g sucrose l(-1) for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium) a maximum yield of 11 g dry biomass l(-1) was achieved after 60 days. However, the amount of total phenolics (57 mg g(-1) DW), flavonoids (34 mg g(-1) DW) and caffeic acid derivatives (38 mg g(-1) DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g(-1) DW, 22 mg chichoric acid g(-1) DW and 4 mg caftaric acids g(-1) DW were achieved with adventitious roots grown in 1,000 l bioreactors.
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【Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid.].
【在空运生物反应器中大规模种植紫锥菊的不定根，用于生产Chichoricic酸，绿原酸和Caftaric酸。】
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DOI：10.1007/s10529-007-9399-1文章类型：杂志文章
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：紫锥菊的次生根在装有2 mg吲哚丁酸l（-1）的Murashige和Skoog（MS）培养基中于气举生物反应器（20 l，500 l气球型，气泡生物反应器和1,000 l鼓型气泡生物反应器）中培养。 ）和50克蔗糖l（-1）用于生产手性酸，绿原酸和异戊酸。 60天后，在20升生物反应器（包含14升MS培养基）中，最大产量为11克干燥生物质l（-1）。但是，总酚含量（57 mg g（-1）DW），类黄酮（34 mg g（-1）DW）和咖啡酸衍生物（38 mg g（-1）DW）的量在50天后最高。基于这些研究，建立了中试规模的培养，分别在500 l和1,000 l生物反应器中实现了3.6 kg和5.1 kg干燥生物量。 5 mg绿原酸g（-1）DW，22 mg chichoric acid g（-1）DW和4 mg caftaric acid g（-1）DW的积累是通过在1,000升生物反应器中生长的不定根实现的。
