Professional antigen-presenting cells (APC) are able to process and present exogenous antigen leading to the activation of T cells. Antigen-immunoglobulin (Ig)G complexes (IC) are much more efficiently processed and presented than soluble antigen. Dendritic cells (DC) are known for their ability to take up and process immune complex (IC) via FcgammaR, and they have been shown to play a crucial role in IC-processing onto major histocompatibility complex (MHC) class I as they contain a specialized cross-presenting transport system required for MHC class I antigen-processing. However, the MHC class II-antigen-processing pathway is distinct. Therefore various other professional APC, like macrophages and B cells, all displaying FcgammaR, are thought to present IC-delivered antigen in MHC class II. Nonetheless, the relative contribution of these APC in IC-facilitated antigen-presentation for MHC class II in vivo is not known. Here we show that, in mice, both macrophages and DC, but not B cells, efficiently capture IC. However, only DC, but not macrophages, efficiently activate antigen-specific MHC class II restricted CD4(+) T cells. These results indicate that mainly DC and not other professional APC, despite expressing FcgammaR and MHC class II, contribute significantly to IC-facilitated T cell activation in vivo under steady-state conditions.

译文

:专业抗原呈递细胞(APC)能够处理并呈递导致T细胞活化的外源性抗原。抗原-免疫球蛋白(Ig)G复合物(IC)比可溶性抗原的加工和呈递效率更高。树突状细胞(DC)以其通过FcgR吸收并处理免疫复合物(IC)的能力而闻名,并且由于它们包含I型树突状细胞(DC),它们在IC处理I类主要组织相容性复合物(MHC)中起着至关重要的作用。 MHC I类抗原加工所需的专业交叉展示转运系统。但是,MHC II类抗原加工途径是不同的。因此,所有其他均显示FcγR的其他专业APC(如巨噬细胞和B细胞)被认为在II类MHC中呈递IC传递的抗原。然而,尚不清楚这些APC在体内II型MHC的IC促进的抗原呈递中的相对贡献。在这里,我们表明,在小鼠中,巨噬细胞和DC都有效捕获了IC,而B细胞却没有。但是,只有DC,而不是巨噬细胞,可以有效地激活II型MHC限制性抗原特异性CD4()T细胞。这些结果表明,尽管表达FcgammaR和II类MHC,主要还是DC,而不是其他专业APC,在稳态条件下在体内促进了IC促进的T细胞活化。

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