PURPOSE:Panax notoginseng is a commonly used Chinese herb. Although a few studies have found that notoginseng shows anti-tumor effects, the effect of this herb on colorectal cancer cells has not been investigated. 5-Fluorouracil (5-FU) is a chemotherapeutic agent for the treatment of colorectal cancer that interferes with the growth of cancer cells. However, this compound has serious side effects at high doses. In this study, using HCT-116 human colorectal cancer cell line, we investigated the possible synergistic anti-cancer effects between notoginseng flower extract (NGF) and 5-FU on colon cancer cells. METHODS:The anti-proliferation activity of these modes of treatment was evaluated by MTS cell proliferation assay. Apoptotic effects were analyzed by using Hoechst 33258 staining and Annexin-V/PI staining assays. The anti-proliferation effects of four major single compounds from NGF, ginsenosides Rb1, Rb3, Rc and Rg3 were also analyzed. RESULTS:Both 5-FU and NGF inhibited proliferation of HCT-116 cells. With increasing doses of 5-FU, the anti-proliferation effect was slowly increased. The combined usage of 5-FU 5 microM and NGF 0.25 mg/ml, significantly increased the anti-proliferation effect (59.4 +/- 3.3%) compared with using the two medicines separately (5-FU 5 microM, 31.1 +/- 0.4%; NGF 0.25 mg/ml, 25.3 +/- 3.6%). Apoptotic analysis showed that at this concentration, 5-FU did not exert an apoptotic effect, while apoptotic cells induced by NGF were observed, suggesting that the anti-proliferation target(s) of NGF may be different from that of 5-FU, which is known to inhibit thymidilate synthase. CONCLUSIONS:This study demonstrates that NGF can enhance the anti-proliferation effect of 5-FU on HCT-116 human colorectal cancer cells and may decrease the dosage of 5-FU needed for colorectal cancer treatment.

译文

目的:三七是一种常用的中草药。尽管一些研究发现三七具有抗肿瘤作用,但尚未研究该药对结肠直肠癌细胞的作用。 5-氟尿嘧啶(5-FU)是一种治疗结肠直肠癌的化学治疗剂,它会干扰癌细胞的生长。但是,这种化合物在高剂量时具有严重的副作用。在这项研究中,我们使用HCT-116人结肠直肠癌细胞系,研究了三七花提取物(NGF)和5-FU对结肠癌细胞可能的协同抗癌作用。
方法:通过MTS细胞增殖试验评估了这些治疗方式的抗增殖活性。通过使用Hoechst 33258染色和膜联蛋白-V / PI染色分析来分析细胞凋亡作用。还分析了NGF中的四种主要单一化合物,人参皂甙Rb1,Rb3,Rc和Rg3的抗增殖作用。
结果:5-FU和NGF均抑制HCT-116细胞的增殖。随着5-FU剂量的增加,抗增殖作用缓慢增加。与分别使用两种药物(5-FU 5 microM,31.1 /-0.4%)相比,5-FU 5 microM和NGF 0.25 mg / ml的联合使用显着提高了抗增殖效果(59.4 /-3.3%); NGF 0.25 mg / ml,25.3±3.6%)。凋亡分析表明,在此浓度下,5-FU不会发挥凋亡作用,而观察到NGF诱导的凋亡细胞,这表明NGF的抗增殖靶标可能与5-FU的不同。已知抑制胸苷酸合酶。
结论:本研究表明NGF可以增强5-FU对HCT-116人结肠直肠癌细胞的抗增殖作用,并可以减少5-FU治疗结肠直肠癌所需的剂量。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录