Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing 1/2 MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 degrees C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on 1/2 MS medium with 0.1M sucrose and 0.7% percent agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
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【Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration.].
【低温保存文心兰模拟物的种子和离体培养的原球茎。 （兰科）通过包囊脱水。】
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：包囊脱水用于冷冻保存文心兰的种子和体外培养的原球茎。授粉后120天，将新鲜收获的种子封装在含有1/2 MS培养基（含3％蔗糖和3％海藻酸钙）的珠子中，然后在补充有0.15 M蔗糖的搅拌（80 rpm）液体培养基中进行预处理（24 h）， 0.25 M蔗糖（48 h），0.5 M蔗糖（24 h）和0.75 M蔗糖（24 h）。带有种子的珠子用硅胶脱水5 h至19.2％的水分含量，并浸入液氮中1 h，在30摄氏度下解冻2分钟，使用相同系列的液体培养基[0.5 M蔗糖（ 24小时），0.25 M蔗糖（48小时），0.15 M蔗糖（24小时）]，并在含0.1M蔗糖和0.7％琼脂的1/2 MS培养基上重新培养。冷冻保存的种子中有多达4.8％产生了完整的植物。按照相同的程序成功地冷冻保存了体外培养的原球茎，使其中的11.3％可以生产植物。
