Stress proteins of the Hsp70 family induced in the blue mussel Mytilus edulis exposed in the laboratory to increased concentrations of cadmium (Cd) or tributhyltin (TBT) were analysed using Western blotting and/or ELISA tests. Statistical evaluation of results indicated that increased concentrations of Hsp70 were detected by means of the ELISA tests as compared with control organisms in extracts from the gills of mussels exposed to both Cd or TBT (p = 0.022). Results of analysis by means of Western blotting showed no differences in the levels of Hsp70 in the extracts (p = 0.151). It was concluded that the ELISA test allowed a more sensitive detection of Hsp70 than did Western blotting.

译文

:使用蛋白质印迹和/或ELISA测试分析了在实验室中暴露于浓度升高的镉(Cd)或三丁锡(TBT)的蓝贻贝蓝色贻贝中诱导的Hsp70家族的应力蛋白。结果的统计评估表明,与暴露于Cd或TBT的贻贝the提取物中的对照生物相比,通过ELISA试验检测到的Hsp70浓度升高(p = 0.022)。通过蛋白质印迹分析的结果表明,提取物中Hsp70的水平没有差异(p = 0.151)。结论是,与蛋白质印迹法相比,ELISA试验可以更敏感地检测Hsp70。

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