The antigens in normal human skin defined by antibodies in patients with bullous pemphigoid (BP) were studied by Western immunoblots. Eighteen (90%) of 20 BP sera reacted to a 230-kD antigen. Seven (35%) of the sera reacted to a 160-kD antigen. Two of these reacted only to the 160-kD antigen and five also reacted to the 230-kD antigen. Antibodies to the 160-kD antigen were not present in 25 control sera obtained from normal individuals or patients with other bullous diseases. The 160-kD antigen was present in epidermal extracts of four different specimens of normal human skin but not in dermal extracts or extracts of control cells including melanoma, fibroblasts, lung carcinoma, and colon carcinoma. Monospecific sera with antibodies to either the 230-kD or to the 160-kD antigen reacted solely to their respective target antigens, but not to both, in extracts of epidermis that contained both antigens. The 160-kD antigen broke down to a 140-kD fragment, while the 230-kD antigen was unchanged in the absence of protease inhibitors. Western blot affinity purified antibody to the 160-kD antigen bound only to the basement membrane zone on the epidermal side of 1M NaCl split skin. These results indicate that a 160-kD antigen is a normal component of the basement membrane zone of human skin. The antigen is located on the epidermal side of skin split with 1M NaCl. It is a minor BP antigen, antibodies to which are present in some patients with BP.

译文

通过Western免疫印迹研究了大疱性类天疱疮 (BP) 患者中由抗体定义的正常人皮肤中的抗原。20个BP血清中的18个 (90%) 对230-kD抗原反应。七个 (35%) 的血清反应至160-kD抗原。其中两个仅对160-kD抗原反应,五个也对230-kD抗原反应。在从正常个体或其他大疱性疾病患者获得的对照血清中不存在针对160-kD抗原的抗体。160-kD抗原存在于正常人皮肤的四个不同标本的表皮提取物中,但不存在于真皮提取物或对照细胞的提取物中,包括黑色素瘤、成纤维细胞、肺癌和结肠癌。在含有两种抗原的表皮提取物中,具有针对230-kD或针对160-kD抗原的抗体的单特异性血清仅与它们各自的靶抗原反应,但不与两者反应。160-kD抗原分解为140-kD片段,而230-kD抗原在没有蛋白酶抑制剂的情况下不变。Western印迹亲和纯化的160-kD抗原抗体仅与1mnacl分裂皮肤的表皮侧的基底膜区结合。这些结果表明160-kD抗原是人皮肤基底膜区的正常成分。抗原位于1M NaCl分裂的皮肤的表皮侧。它是一种次要的BP抗原,在某些BP患者中存在抗体。

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