BACKGROUND:Flow cytometric basophil activation tests have been developed as cellular tests for in vitro diagnosis of IgE-mediated reactions. Different activation markers (CD63 or CD203c) with distinct ways of regulation have been used after stimulation with various allergens. OBJECTIVE:It was the aim of the present study to compare basophil activation tests by measuring both CD63 and CD203c upregulation in patients with insect venom allergy. MATERIALS AND METHODS:43 patients with a history of insect venom anaphylaxis were examined. A careful allergy history was taken, and skin tests and determination of specific IgE-antibodies were performed. Basophil activation tests (BAT) using CD63 or CD203c expression were done after stimulation with different concentrations of bee and wasp venom extracts. 25 healthy subjects with negative history of insect venom allergy were studied as controls. RESULTS:The CD203c protocol showed a slightly higher sensitivity than the CD63 protocol (97% vs. 89%) with regard to patients' history. The magnitude of basophil response was higher with CD203c in comparison to CD63 for both insect venoms. Specificity was 100% for the CD63 protocol and 89% for the CD203c protocol with regard to controls with negative history and negative RAST. CONCLUSION:These results support the reliability of basophil activation tests using either CD63 or CD203c as cellular tests in the in vitro diagnosis of patients with bee or wasp venom allergy with a slightly higher sensitivity for the CD203c protocol.

译文

背景:流式细胞仪嗜碱性粒细胞活化测试已发展为用于体外诊断IgE介导的反应的细胞测试。在用各种变应原刺激后,已使用具有不同调节方式的不同激活标记(CD63或CD203c)。
目的:本研究的目的是通过测量昆虫毒液过敏患者中的CD63和CD203c上调来比较嗜碱性粒细胞活化测试。
材料与方法:检查43例有虫毒过敏史的患者。仔细记录过敏史,并进行皮肤测试和特定IgE抗体的测定。在用不同浓度的蜜蜂和黄蜂毒液提取物刺激后,进行了使用CD63或CD203c表达的嗜碱性粒细胞活化测试(BAT)。以25名昆虫毒液过敏史为阴性的健康受试者作为对照。
结果:就患者病史而言,CD203c方案显示出比CD63方案更高的敏感性(97%比89%)。与两种昆虫毒液的CD63相比,CD203c的嗜碱性粒细胞反应程度更高。对于具有阴性历史和阴性RAST的对照,CD63方案的特异性为100%,CD203c方案的特异性为89%。
结论:这些结果支持使用CD63或CD203c作为细胞测试的嗜碱性粒细胞活化测试在对蜜蜂或黄蜂毒液过敏患者进行体外诊断时的可靠性,对CD203c协议的敏感性稍高。

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