• 【单卵泡体外受精中的卵泡冲洗几乎使可转移胚胎的数量增加一倍。】 复制标题 收藏 收藏
    DOI:10.1111/aogs.12054 复制DOI
    作者列表:von Wolff M,Hua YZ,Santi A,Ocon E,Weiss B
    BACKGROUND & AIMS: :Follicle flushing has been proved to be ineffective in polyfollicular in vitro fertilization. To analyze the effect of flushing in monofollicular in vitro fertilization we aspirated and then flushed the follicles in 164 cycles. Total oocyte yield/aspiration was 44.5% in the aspirate, 20.7% in the 1(st) flush, 10.4% in the 2(nd) flush and 4.3% in the 3(rd) flush. By flushing, the total oocyte yield increased (p < 0.01) by 80.9%, from 44.5 to 80.5%. The total transfer rate increased (p < 0.01) by 91.0%, from 20.1 to 38.4%. The results indicate that the oocyte yield and the number of transferable embryos can be increased significantly by flushing.
    背景与目标: :卵泡冲洗已被证明在多卵泡体外受精中无效。为了分析潮红在单卵体外受精中的作用,我们吸出并随后在164个周期中冲洗了卵泡。抽出物中的卵母细胞总产量/抽吸率为44.5%,在第1次冲洗中为20.7%,在第2次冲洗中为10.4%,在第3次冲洗中为4.3%。通过冲洗,卵母细胞总产量从44.5%增至80.5%(p <0.01),增幅为80.9%。总传输率从20.1增加到38.4%,增幅(p <0.01)91.0%。结果表明通过冲洗可以显着提高卵母细胞的产量和可转移胚胎的数量。
  • 【体外受精后选择性单胚胎移植的有效多层面实施策略的评估。】 复制标题 收藏 收藏
    DOI:10.1093/humrep/des371 复制DOI
    作者列表:Kreuwel IA,van Peperstraten AM,Hulscher ME,Kremer JA,Grol RP,Nelen WL,Hermens RP
    BACKGROUND & AIMS: STUDY QUESTION:What is the relationship between the rate of elective single-embryo transfer (eSET) and couples' exposure to different elements of a multifaceted implementation strategy? SUMMARY ANSWER:Additional elements in a multifaceted implementation strategy do not result in an increased eSET rate. WHAT IS KNOWN ALREADY:A multifaceted eSET implementation strategy with four different elements is effective in increasing the eSET rate by 11%. It is unclear whether every strategy element contributes equally to the strategy's effectiveness. STUDY DESIGN AND SIZE:An observational study was performed among 222 subfertile couples included in a previously performed randomized controlled trial. PARTICIPANTS, SETTINGS AND METHODS:Of the 222 subfertile couples included, 109 couples received the implementation strategy and 113 couples received standard IVF care. A multivariate regression analysis assessed the effectiveness of four different strategy elements on the decision about the number embryos to be transferred. Questionnaires evaluated the experiences of couples with the different elements. MAIN RESULTS AND ROLE OF CHANCE:Of the couples who received the implementation strategy, almost 50% (52/109) were exposed to all the four elements of the strategy. The remaining 57 couples who received two or three elements of the strategy could be divided into two further classes of exposure. Our analysis demonstrated that additional elements do not result in an increased eSET rate. In addition to the physician's advice, couples rated a decision aid and a counselling session as more important for their decision to transfer one or two embryos, compared with a phone call and a reimbursement offer (P < 0.001). LIMITATIONS AND REASONS FOR CAUTION:The differences in eSET rate between exposure groups failed to reach significance, probably because of the small numbers of couples in each exposure group. WIDER IMPLICATIONS OF THE FINDINGS:Adding more elements to an implementation strategy does not always result in an increased effectiveness, which is in concordance with recent literature. This in-depth evaluation of a multifaceted intervention strategy could therefore help to modify strategies, by making them more effective and less expensive.
    背景与目标: 研究问题:选择性单胎移植率(eSET)与夫妇对多层面实施策略不同要素的接触程度之间有什么关系?
    概要解答:多方面实施策略中的其他元素不会导致eSET率提高。
    已经知道的:具有四个不同元素的多层面eSET实施策略可以有效地将eSET率提高11%。尚不清楚每个策略要素是否均对策略的有效性做出同等贡献。
    研究设计和规模:一项观察性研究是对先前进行的随机对照试验中包括的222对亚生育夫妇进行的。
    参加者,地点和方法:在包括的222对不育夫妇中,有109对夫妇接受了实施策略,对113对夫妇接受了标准的IVF护理。多元回归分析评估了关于决定移植胚胎数量的四个不同策略要素的有效性。问卷评估了具有不同元素的夫妻的经历。
    主要结果和机会:在接受实施策略的夫妇中,几乎50%(52/109)接触了该策略的所有四个要素。剩下的接受了该策略的两个或三个要素的57对夫妇可以分为两类。我们的分析表明,其他元素不会导致eSET速率增加。除了医师的建议外,夫妻俩认为,与电话和报销相比,决定协助和咨询对他们决定转移一个或两个胚胎更为重要(P <0.001)。
    注意事项的局限性和原因:暴露组之间的eSET率差异未能达到显着水平,这可能是因为每个暴露组中的夫妻数量很少。
    结果的更广泛含义:将更多元素添加到实施策略中并不一定会导致有效性提高,这与最近的文献一致。因此,对多方面干预策略的这种深入评估可以使策略更有效,更便宜,从而有助于修改策略。
  • 【附睾液和睾丸匀浆中荷斯坦公牛精子中骨桥蛋白的检测及其在牛受精中的潜在作用。】 复制标题 收藏 收藏
    DOI:10.1530/REP-06-0228 复制DOI
    作者列表:Erikson DW,Way AL,Chapman DA,Killian GJ
    BACKGROUND & AIMS: :Osteopontin (OPN) is a secreted extracellular matrix phosphoprotein identified in various tissues and fluids including those of the male and female reproductive tracts. OPN was previously identified as a 55 kDa high fertility marker in Holstein bull seminal plasma, produced by the ampulla and the vesicular gland. The objectives of this study were to characterize OPN on ejaculated and cauda epididymal sperm using immunofluorescence and western blot analysis, and to assess the role of sperm OPN in fertilization. Solubilized sperm membrane proteins from ejaculated and cauda epididymal sperm were separated by 1D SDS-PAGE, transferred to nitrocellulose, and probed with an antibody to bovine milk OPN. A 35 kDa protein was detected by this antibody in both ejaculated and cauda epididymal sperm membranes. Analyses also recognized OPN at 55 and 25 kDa in cauda epididymal fluid and testicular parenchyma homogenates respectively. Immunofluorescent analysis of ejaculated and cauda epididymal sperm showed OPN localization in a well-defined band in the postacrosomal region of the sperm head and also on the midpiece. Results of in vitro fertilization experiments showed that sperm treated with an antibody to OPN fertilized fewer oocytes than sperm treated with control medium while increasing incidence of polyspermy, suggesting a role of sperm-associated OPN in fertilization and a block to polyspermy. These studies demonstrate that OPN exists at multiple molecular weight forms in the bull reproductive tract and its presence on ejaculated sperm may signal its importance in fertilization by interacting with integrins or other proteins on the oocyte plasma membrane.
    背景与目标: :骨桥蛋白(OPN)是一种分泌的细胞外基质磷蛋白,可在各种组织和体液(包括男性和女性生殖道的组织和体液)中鉴定出。在壶腹和水泡腺产生的荷斯坦公牛精浆中,OPN先前被鉴定为55 kDa的高生育力标记。这项研究的目的是使用免疫荧光和蛋白质印迹分析来表征射精和马尾附睾精子上的OPN,并评估精子OPN在受精中的作用。通过1D SDS-PAGE分离来自射精和马尾附睾精子的可溶性精子膜蛋白,将其转移到硝酸纤维素膜上,并用针对牛乳OPN的抗体进行探测。用此抗体在射精和马尾附睾精子膜中检测到35 kDa的蛋白质。分析还分别在马尾附睾液和睾丸薄壁组织匀浆中分别以55和25 kDa的浓度识别了OPN。射精和马尾附睾精子的免疫荧光分析显示,OPN定位在精子头部顶体后区域以及中段的明确定义的条带中。体外受精实验的结果表明,用抗OPN抗体处理的精子受精卵比用对照培养基处理的精子少,同时增加了多精子的发生率,这表明与精子相关的OPN在受精中的作用和对多精子的阻滞。这些研究表明,OPN在牛的生殖道中以多种分子量形式存在,并且其在精子上的存在可能通过与卵母细胞质膜上的整联蛋白或其他蛋白质相互作用来表明其在受精中的重要性。
  • 【海胆中的线粒体调控。 I.线粒体超微结构的转变和受精时ADP:ATP比率的变化。】 复制标题 收藏 收藏
    DOI:10.1016/0014-4827(76)90461-4 复制DOI
    作者列表:Innis MA,Beers TR,Craig SP
    BACKGROUND & AIMS: -2
    背景与目标: -2
  • 【定义体外受精成功率的三个简单指标。】 复制标题 收藏 收藏
    DOI:10.1016/j.fertnstert.2020.04.056 复制DOI
    作者列表:Fischer C,Scott RT Jr
    BACKGROUND & AIMS: :Describing clinical outcomes from assisted reproduction technology (ART) treatment cycles has been an evolving challenge throughout the world. Three simple metrics provide a transparent and highly accurate summary of ART outcomes. The first metric is the probability of having no embryos available to transfer. This metric incorporates all causes of failure from initiation of the treatment cycle up to the point immediately before actual embryo transfer. Patients will know what the risk is of failing, whether it is due to poor follicular stimulation, failed fertilization, poor embryo development, or abnormal preimplantation genetic testing for aneuploidy (PGT-A) results. The second and most important metric is sustained implantation rate: the probability that any transferred embryo will implant and progress to delivery. In the event of a single-embryo transfer, the metric is identical to delivery rate per transfer. By calculating per embryo, it provides a summary of the quality of outcomes within the program without the obscuring effect of multiple-embryo transfer. The final metric is the number of supernumerary embryos cryopreserved during the cycle. This speaks to the efficiency of the process by providing an estimate of potential benefits which may come from an additional transfer should the first one be unsuccessful or even to allow the couple to pursue an additional child without another full ART cycle. These metrics are easy to calculate and provide a detailed picture of the outcomes attained by the program.
    背景与目标: :描述辅助生殖技术(ART)治疗周期的临床结果一直是世界范围内不断发展的挑战。三个简单的指标可提供ART结果的透明且高度准确的摘要。第一个指标是没有胚胎可转移的概率。该指标涵盖了从治疗周期开始到实际胚胎移植之前的所有失败原因。患者将知道失败的风险是什么,无论是由于不良的卵泡刺激,受精失败,胚胎发育不良还是非整倍性(PGT-A)结果的植入前基因测试异常。第二个也是最重要的指标是持续着床率:任何转移的胚胎将着床并继续进行分娩的概率。如果是单胚胎移植,则该度量标准与每次转移的交付率相同。通过计算每个胚胎,它提供了该程序中结果质量的摘要,而没有多胚胎移植的影响。最终指标是在周期中冷冻保存的多余胚胎的数量。通过提供一个可能的收益的估计,可以证明该过程的效率,如果第一次转移失败或什至允许夫妇在没有另一个完整的抗逆转录病毒治疗周期的情况下继续寻找另一个孩子,可能会从一次额外的转移中获得潜在的利益。这些度量标准易于计算,并提供程序所达到的结果的详细信息。
  • 【组织学成分定量,用于评估自然周期接受体外受精/胞浆内精子注射的女性子宫内膜容受性。】 复制标题 收藏 收藏
    DOI:10.1016/j.tjog.2017.04.019 复制DOI
    作者列表:Wang DY,Tian L,Shen D,Yang ZJ
    BACKGROUND & AIMS: OBJECTIVE:Our aim was to evaluate the value of the volumetric fraction of vascular endothelial cells (EnVF) for determining endometrial receptivity in women undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). MATERIALS AND METHODS:The records of women undergoing IVF/ICSI between 2006 and 2010 were retrospectively reviewed. An endometrial biopsy was performed in the cycle prior to IVF/ICSI. EnVF was calculated from endometrial biopsy staining. RESULTS:Twenty-seven patients who did not become pregnant, 8 who had a miscarriage, and 21 with a clinical pregnancy were included. The three groups were similar with respect to infertility and IVF characteristics. An EnVF ≤3.85 was associated with not becoming pregnant, an EnVF >5.29 with miscarriage, and a level between 3.86 and 5.29 was associated with clinical pregnancy (p = 0.001). CONCLUSIONS:EnVF examined in the prior cycle may be a marker of endometrial receptivity and predict the chance of pregnancy in women undergoing IVF/ICSI.
    背景与目标: 目的:我们的目的是评估血管内皮细胞(EnVF)的体积分数对确定体外受精/胞浆内精子注射(IVF / ICSI)妇女的子宫内膜容受性的价值。
    材料与方法:回顾性分析了2006年至2010年间进行IVF / ICSI的妇女的记录。在IVF / ICSI之前的周期中进行子宫内膜活检。从子宫内膜活检染色计算EnVF。
    结果:27例未怀孕,8例流产,21例临床妊娠。这三组在不育和IVF特征方面相似。 EnVF≤3.85与未怀孕有关,EnVF> 5.29与流产有关,而3.86至5.29之间的水平与临床妊娠有关(p = 0.001)。
    结论:前一个周期检查的EnVF可能是子宫内膜接受性的标志,并预测接受IVF / ICSI的妇女怀孕的机会。
  • 【抗谱蛋白抗体会污染斑点豆(Ancos)卵中的卵母细胞核和受精通道位点。】 复制标题 收藏 收藏
    DOI:10.1002/mrd.1080260207 复制DOI
    作者列表:Campanella C,Carotenuto R,Gabbiani G
    BACKGROUND & AIMS: :In Discoglossus pictus eggs, only the dimple contains ionic channels active at fertilization; in particular, chloride channels are found in the central portion of the dimple, which is also the site of sperm penetration. Moreover the dimple hosts an imposing cytoskeleton, consisting of a cortical network and bundles of microfilaments extending from the microvilli. Since spectrin cross links actin and is connected through ankyrin to anion transporters in the plasma membrane of erythrocytes as well as to anion channels in other cells, we studied, in D. pictus egg, the relationship between the localization of spectrin and the high polarization of ionic channels and cytoskeletal organization. By means of immunocytochemistry, we localized spectrin exclusively in the egg dimple. In an attempt to trace back the source of spectrin localization, we immunostained sections of D. pictus ovary and localized spectrin in the nuclei of previtellogenic oocytes, where actin is also present. Antispectrin staining remained until germinal vesicle breakdown. By contrast, a cortical localization was found only when the oocytes divided into two hemispheres and into the germinative area (GA), which, after germinal vesicle breakdown, gives rise to the dimple. At this stage the antispectrin signal was particularly strong in the GA. Using Rho-pialloidin, we also established that spectrin is generally present where F-actin is found. However, spectrin and F-actin do not have the same pattern of fluorescence. In conclusion, our data suggest that spectrin may play a role in oocyte and egg polarity. In eggs, it could be instrumental in anchoring to the cytoskeleton membrane proteins such as receptors and ionic channels, including chloride-permeable channels.
    背景与目标: :在盘pic蛋中,只有酒窝中含有在受精时活跃的离子通道;特别地,在酒窝的中央部分发现了氯离子通道,这也是精子穿透的部位。此外,酒窝有一个气势旺盛的细胞骨架,由皮质网络和从微绒毛延伸出来的微丝束组成。由于血影蛋白交联肌动蛋白并通过锚蛋白连接至红细胞质膜中的阴离子转运蛋白以及其他细胞中的阴离子通道,因此我们在D. pictus卵中研究了血影蛋白的定位与高蛋白极化之间的关系。离子通道和细胞骨架组织。通过免疫细胞化学,我们将血影蛋白仅定位在蛋酒窝中。为了追溯血影蛋白定位的来源,我们对D. pictus卵巢的切片进行了免疫染色,并在前玻璃化卵母细胞的核中定位了血影蛋白,那里也存在肌动蛋白。保留抗谱蛋白染色直至生发囊泡破裂。相比之下,仅当卵母细胞分为两个半球并进入发芽区域(GA)时才发现皮层定位,在发芽囊泡破裂后会形成酒窝。在此阶段,GA中的抗谱蛋白信号特别强。使用Rho-pialloidin,我们还确定在发现F-肌动蛋白的地方通常存在血影蛋白。然而,血影蛋白和F-肌动蛋白没有相同的荧光模式。总之,我们的数据表明血影蛋白可能在卵母细胞和卵的极性中起作用。在卵中,它可能有助于锚定细胞骨架膜蛋白,例如受体和离子通道,包括氯离子可渗透通道。
  • 【睾丸精子提取程序和体外受精-胞浆内精子注射结果的时间选择。】 复制标题 收藏 收藏
    DOI:10.1016/s0015-0282(01)01908-2 复制DOI
    作者列表:Levran D,Ginath S,Farhi J,Nahum H,Glezerman M,Weissman A
    BACKGROUND & AIMS: OBJECTIVE:To compare the outcome of IVF-intracytoplasmic sperm injection (ICSI) using testicular spermatozoa obtained on the day of ovum pick-up (OPU) or on the day before OPU. DESIGN:Retrospective study. SETTING:An IVF clinic in a university hospital. PATIENT(S):Forty-seven IVF-ICSI cycles using testicular spermatozoa in 28 couples with the male partner suffering from nonobstructive azoospermia. INTERVENTION(S):Sperm retrieval was performed either on the OPU day (23 cycles in 19 patients; group A) or on the day before OPU (24 cycles in 15 patients; group B). Testicular sperm aspiration (TESA) was performed and followed by testicular sperm extraction (TESE) if no spermatozoa could be found. MAIN OUTCOME MEASURE(S):The presence of motile spermatozoa at the time of ICSI and fertilization and clinical pregnancy rates. RESULT(S):A similar proportion of motile spermatozoa (60.9% vs. 62.5%), fertilization rate (61.7% vs. 58.9%), and clinical pregnancy rate per transfer (34.8% and 29.2%) were obtained for groups A and B, respectively. CONCLUSION(S):Testicular sperm retrieval can be performed on the day before OPU without compromising success. Considerable medical and practical advantages may be offered by further advancement of testicular sperm retrieval procedures to 48 hours before OPU. This approach should thus be further evaluated.
    背景与目标: 目的:比较使用卵子取卵日(OPU)或OPU前一天获得的睾丸精子进行IVF-胞浆内精子注射(ICSI)的结果。
    设计:回顾性研究。
    地点:大学医院的试管婴儿诊所。
    患者:28对使用睾丸精子的IVF-ICSI周期为28对,其男性伴侣患有非阻塞性无精子症。
    干预:在OPU当天(19例,23个周期; A组)或OPU注射前一天(15例,24个周期; B组)进行精子取出。进行睾丸精子抽吸(TESA),如果找不到精子,则进行睾丸精子提取(TESE)。
    主要观察指标:ICSI,施肥和临床妊娠率时存在活动精子。
    结果:A组和A组获得了相似比例的活动精子(60.9%对62.5%),受精率(61.7%对58.9%)和每次转移的临床妊娠率(34.8%和29.2%)。 B分别。
    结论:可以在OPU前一天进行睾丸精子取回,而不会影响成功率。通过将睾丸精子取出程序进一步推进到OPU之前48小时,可以提供相当大的医学和实践优势。因此,应进一步评估这种方法。
  • 【对磷肥的响应,形成了多种多样的附生植物群落。】 复制标题 收藏 收藏
    DOI:10.1111/j.1461-0248.2007.01054.x 复制DOI
    作者列表:Benner JW,Vitousek PM
    BACKGROUND & AIMS: :The role of terrestrial soil nutrient supply in determining the composition and productivity of epiphyte communities has been little investigated. In a montane Hawaiian rainforest, we documented dramatic increases in the abundance and species richness of canopy epiphytes in a forest that had been fertilized annually with phosphorus (P) for 15 years; there was no response in forest that had been fertilized with nitrogen (N) or other nutrients. The response of N-fixing lichens to P fertilization was particularly strong, although mosses and non-N-fixing lichens also increased in abundance and diversity. We show that enhancement of canopy P availability is the most likely factor driving the bloom in epiphytes. These results provide strong evidence that terrestrial soil fertility may structure epiphyte communities, and in particular that the abundance of N-fixing lichens--a functionally important epiphyte group--may be particularly sensitive to ecosystem P availability.
    背景与目标: :很少研究陆地土壤养分供应对确定附生植物群落组成和生产力的作用。在夏威夷山区的雨林中,我们记录了每年用磷(P)施肥15年的森林中冠层附生植物的丰度和物种丰富度的急剧增加;在用氮(N)或其他养分施肥的森林中没有反应。固氮地衣对磷肥的响应特别强烈,尽管苔藓和非固氮地衣的丰度和多样性也有所增加。我们表明,冠层P可用性的提高是驱动附生植物开花的最可能因素。这些结果提供了有力的证据,表明陆地土壤肥力可能构成了附生植物群落,尤其是固氮的地衣植物(功能上重要的附生植物群)可能对生态系统磷的可用性特别敏感。
  • 【人体体外受精结果的卵泡液生物标志物:原理证明。】 复制标题 收藏 收藏
    DOI:10.1186/s12953-016-0106-9 复制DOI
    作者列表:Chen F,Spiessens C,D'Hooghe T,Peeraer K,Carpentier S
    BACKGROUND & AIMS: BACKGROUND:Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported. METHODS:This study screened FF for peptide biomarkers that predict the outcome of in vitro fertilization (IVF). Potential biomarkers were discovered by investigating 2 training datasets, consisting both of 17 samples and validating on an independent experiment containing 32 samples. Peptide profiles were acquired by nano-scale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). RESULTS:From the training datasets 53 peptides were found as potential biomarker candidates, predicting the fertilization outcome of 24 out of the 32 validation samples blindly (81.3% sensitivity, 68.8% specificity, AUC = 0.86). Seven potential biomarker peptides were identified. They were derived from: insulin-like growth factor binding protein-5, alpha-2-antiplasmin, complement component 3, inter-alpha-trypsin inhibitor heavy chain H1, serum albumin, protein diaphanous homolog 1 and plastin-3. CONCLUSIONS:The MS-based comprehensive peptidomic approach carried out in this study, established a novel panel of potential biomarkers that present a promising predictive accuracy rate in fertilization outcome, and indicates FF as an interesting biomarker resource to improve IVF clinic routine.
    背景与目标: 背景:人卵泡液(FF)是卵母细胞在体内发育的独特生物液,是非侵入性生化预测因子的最佳来源。卵母细胞的质量直接影响胚胎的发育,因此可以用作胚胎质量的预测指标。 FF的肽谱分析及其作为卵母细胞质量生物标志物的潜在用途尚未见报道。
    方法:本研究从FF中筛选了预测体外受精(IVF)结果的肽生物标志物。通过研究2个训练数据集(包括17个样本)并在包含32个样本的独立实验中进行验证,发现了潜在的生物标志物。通过与串联质谱联用的纳米级液相色谱(纳米LC-MS / MS)获得肽谱。
    结果:从训练数据集中发现了53种肽作为潜在的生物标志物候选物,盲目预测了32个验证样品中24个的受精结果(敏感性为81.3%,特异性为68.8%,AUC = 0.86)。鉴定了七个潜在的生物标志物肽。它们来自:胰岛素样生长因子结合蛋白5,α-2-抗纤溶酶,补体成分3,α-胰蛋白酶间抑制剂重链H1,血清白蛋白,透色同源物1和plastin-3。
    结论:在这项研究中基于质谱的综合肽组学方法,建立了一个潜在的生物标志物的新小组,提出了有希望的受精结果的预测准确率,并表明FF是改善IVF临床常规的有趣的生物标志物资源。
  • 【低体重指数会损害体外受精周期中新转移的活产率:一项针对中国人群的回顾性研究。】 复制标题 收藏 收藏
    DOI:10.1016/j.fertnstert.2016.10.029 复制DOI
    作者列表:Cai J,Liu L,Zhang J,Qiu H,Jiang X,Li P,Sha A,Ren J
    BACKGROUND & AIMS: OBJECTIVE:To evaluate the effects of low body mass index (BMI) on in vitro fertilization (IVF) outcomes in fresh transfer cycles. DESIGN:Retrospective cohort study. SETTING:University-affiliated hospital. PATIENT(S):A total of 4,798 cycles with conventional stimulation and fresh transfer in a single IVF center during the period 2013-2014. Low BMI (<18.5 kg/m2) was defined according to World Health Organization guidelines, and cycles within a normal weight range (18.5-24.9 kg/m2) were used as reference. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Live birth rate per fresh embryo transfer. RESULT(S):Low BMI was associated with reduced live birth rates and increased miscarriage rates compared with normal weight, controlling for important covariates known to influence IVF outcomes. Patient age was the most potent confounder, causing a 10.5% reduction in the odds ratio (OR) for live birth between the groups compared. When an interaction term (age × BMI) was introduced, the OR for live birth was reduced in cycles of those aged ≥35 years compared with cycles of those aged 28-34 years, whereas the change in OR between cycles in those aged <28 and cycles in those aged 28-34 years was insignificant. CONCLUSION(S):Low BMI is associated with negative outcomes in fresh transfer cycles, especially for women of advanced age.
    背景与目标: 目的:评估低体重指数(BMI)对新鲜转移周期中体外受精(IVF)结果的影响。
    设计:回顾性队列研究。
    单位:大学附属医院。
    患者:2013-2014年期间,单个IVF中心总共进行了4,798个周期的常规刺激和新鲜转移。根据世界卫生组织的指南定义了低BMI(<18.5 kg / m2),并以正常体重范围(18.5-24.9 kg / m2)内的周期为参考。
    干预措施:无。
    主要观察指标:每次新鲜胚胎移植的活产率。
    结果:与正常体重相比,低BMI与活产率降低和流产率增加有关,控制了已知影响IVF结果的重要协变量。患者年龄是最有力的混杂因素,导致两组之间活产的比值比(OR)降低10.5%。引入交互作用项(年龄×BMI)时,≥35岁的人的活产儿的OR值比28-34岁的人的活产儿的OR值降低,而<28岁的人的活产儿的OR值变化并且年龄在28-34岁之间的周期微不足道。
    结论:低BMI与新鲜转移周期的负结果有关,尤其是对于高龄女性。
  • 【在体外受精和胞浆内精子注射周期中未受精的人卵母细胞的后期受精:常规授精与ICSI。】 复制标题 收藏 收藏
    DOI:10.1023/a:1009409100941 复制DOI
    作者列表:Park KS,Song HB,Chun SS
    BACKGROUND & AIMS: PURPOSE:The aim of this study was to evaluate the efficacy of intracytoplasmic sperm injection (ICSI) in comparison with conventional reinsemination using fertilization failed oocytes by conventional in vitro fertilization (IVF). METHODS:Oocytes were collected from patients of IVF or ICSI cycles. Patients were grouped by fertilization techniques: group 1: conventional IVF; group 2: reinsemination after conventional IVF failure; group 3: regular ICSI; group 4: 1-day-old ICSI after conventional IVF failure; group 5: 2-day-old ICSI after conventional IVF failure; group 6: re-ICSI after regular ICSI failure. RESULTS:In different insemination groups, normal fertilization rate was higher (P < 0.001) in 1-day-old ICSI (47.1%) and 2-day-old ICSI groups (40.0%) than in reinsemination (14.7%). Abnormal fertilization rate was higher (P < 0.05) in re-ICSI group (21.7%) than any other groups (range: 0-8%). Cleavage rate was higher in 1-day-old (36.7%) and 2-day-old ICSI groups (36.0%) than in reinsemination (5.3%, P < 0.001) or re-ICSI groups (17.4%, P < 0.05). Pregnancy rate was 27.6% and 20.0% in conventional IVF and regular ICSI groups, respectively. However, 1-day-old ICSI (group 4) and 2-day-old ICSI (group 5) were attempted once embryo transfer (ET) but failed pregnancy occurred in each group. CONCLUSIONS:In fertilization failure cycles, late ICSI increases the rate of fertilization and embryonic development and may rescue the completely failed attempt of pregnancy.
    背景与目标: 目的:本研究的目的是评估与通过常规体外受精(IVF)使用受精失败的卵母细胞进行常规重新人工授精相比,胞浆内精子注射(ICSI)的疗效。
    方法:从IVF或ICSI周期患者中收集卵母细胞。通过受精技术对患者进行分组:第一组:常规IVF;第二组:常规IVF。第2组:常规IVF失败后再次人工授精;第三组:常规ICSI;第4组:常规IVF失败后1天大的ICSI;第5组:常规IVF失败后2天大的ICSI;第6组:常规的ICSI故障后重新使用ICSI。
    结果:在不同的授精组中,1天大的ICSI组(47.1%)和2天大的ICSI组(40.0%)的正常受精率高于再授精(14.7%)。 re-ICSI组(21.7%)的异常受精率高于其他任何组(范围:0-8%)(P <0.05)。 1天大(36.7%)和2天大ICSI组(36.0%)的卵裂率高于再受精(5.3%,P <0.001)或re-ICSI组(17.4%,P <0.05) 。常规IVF组和常规ICSI组的妊娠率分别为27.6%和20.0%。然而,一旦胚胎移植(ET)但怀孕失败,每组都尝试使用1天大的ICSI(第4组)和2天大的ICSI(第5组)。
    结论:在受精失败的周期中,晚期ICSI可增加受精和胚胎发育的速度,并可能挽救完全失败的妊娠尝试。
  • 【体外受精后甲氨蝶呤因异位妊娠失败的临床预测。】 复制标题 收藏 收藏
    DOI:10.1007/s10815-016-0861-7 复制DOI
    作者列表:Brady PC,Missmer SA,Farland LV,Ginsburg ES
    BACKGROUND & AIMS: PURPOSE:The aim of this study is to investigate the clinical predictors of failure of a single dose of methotrexate (MTX) for management of ectopic pregnancy after in vitro fertilization (IVF). METHODS:A retrospective cohort study was performed of women who conceived ectopic pregnancies following fresh or frozen IVF cycles at an academic infertility clinic between 2007 and 2014, and received intramuscular MTX (50 mg/m2). Successful single-dose MTX treatment was defined as a serum beta-human chorionic gonadotropin (hCG) decline ≥15% between days 4 and 7 post-treatment. Logistic regression models adjusted for oocyte age, number of embryos transferred, and prior ectopic pregnancy were used to estimate the adjusted odds ratio (OR) (95% confidence interval [CI]) of failing one dose of MTX. RESULTS:Sixty-four patients with ectopic pregnancies after IVF were included. Forty required only one dose of MTX (62.5%), while 15 required additional MTX alone (up to four total doses, 23.4%), and 9 required surgery (14.1%). By multivariable logistic regression, the highest tertiles of serum hCG at peak (≥499 IU/L, OR = 9.73, CI 1.88-50.25) and at first MTX administration (≥342 IU/L, OR = 4.74, CI 1.11-20.26), fewer embryos transferred (OR = 0.37 per each additional embryo transferred, CI 0.19-0.74), and adnexal mass by ultrasound (OR = 3.65, CI 1.10-12.11) were each correlated with greater odds of requiring additional MTX and/or surgery. CONCLUSION:This is the first study to report that in women with ectopic pregnancies after IVF, higher hCG-though well below treatment failure thresholds previously described in spontaneous pregnancies-fewer embryos transferred, and adnexal masses are associated with greater odds of failing one dose of MTX. These findings can be used to counsel IVF patients regarding the likelihood of success with single-dose MTX.
    背景与目标: 目的:本研究的目的是研究单剂量甲氨蝶呤(MTX)在体外受精(IVF)后处理异位妊娠失败的临床预测指标。
    方法:一项回顾性队列研究是对2007年至2014年间在学术不育诊所接受新鲜或冷冻IVF周期后异位妊娠并接受肌内MTX(50 mg / m2)的女性进行的。成功的单剂量MTX治疗定义为治疗后第4天至第7天血清β-人绒毛膜促性腺激素(hCG)下降≥15%。使用针对卵母细胞年龄,转移的胚胎数和先前的异位妊娠进行调整的逻辑回归模型,以评估一剂MTX失败的调整后的优势比(OR)(95%置信区间[CI])。
    结果:纳入IVF后异位妊娠的64例患者。四十只只需要一剂MTX(62.5%),而十五只需要单独的MTX(最多四剂,占总剂量的23.4%),九只需要手术(占14.1%)。通过多变量logistic回归分析,血清hCG的最高三分位数在峰值(≥499IU / L,OR = 9.73,CI 1.88-50.25)和首次MTX给药时(≥342IU / L,OR = 4.74,CI 1.11-20.26) ,更少的胚胎被移植(每增加一个胚胎,OR = 0.37,CI 0.19-0.74)和超声附件质量(OR = 3.65,CI 1.10-12.11)都与需要额外MTX和/或手术的几率相关。
    结论:这是第一项报道IVF后异位妊娠妇女的hCG较高,尽管其远低于自发妊娠所描述的治疗失败阈值,但胚胎移植却少了,而附件包块与一次剂量的失败失败的可能性更大。 MTX。这些发现可用于就单剂量MTX成功的可能性向IVF患者提供咨询。
  • 【青藏高原高寒草原丛枝菌根真菌群落对短期施肥的快速反应。】 复制标题 收藏 收藏
    DOI:10.7717/peerj.2226 复制DOI
    作者列表:Xiang X,Gibbons SM,He JS,Wang C,He D,Li Q,Ni Y,Chu H
    BACKGROUND & AIMS: BACKGROUND:The Qinghai-Tibet Plateau (QTP) is home to the vast grassland in China. The QTP grassland ecosystem has been seriously degraded by human land use practices and climate change. Fertilization is used in this region to increase vegetation yields for grazers. The impact of long-term fertilization on plant and microbial communities has been studied extensively. However, the influence of short-term fertilization on arbuscular mycorrhizal fungal (AMF) communities in the QTP is largely unknown, despite their important functional role in grassland ecosystems. METHODS:We investigated AMF community responses to three years of N and/or P addition at an experimental field site on the QTP, using the Illumina MiSeq platform (PE 300). RESULTS:Fertilization resulted in a dramatic shift in AMF community composition and NP addition significantly increased AMF species richness and phylogenetic diversity. Aboveground biomass, available phosphorus, and NO3 (-) were significantly correlated with changes in AMF community structure. Changes in these factors were driven by fertilization treatments. Thus, fertilization had a large impact on AMF communities, mediated by changes in aboveground productivity and soil chemistry. DISCUSSION:Prior work has shown how plants often lower their reliance on AMF symbioses following fertilization, leading to decrease AMF abundance and diversity. However, our study reports a rise in AMF diversity with fertilization treatment. Because AMF can provide stress tolerance to their hosts, we suggest that extreme weather on the QTP may help drive a positive relationship between fertilizer amendment and AMF diversity.
    背景与目标: 背景:青藏高原(QTP)是中国广阔的草原所在地。人们的土地使用方式和气候变化已严重破坏了QTP草原生态系统。在该地区使用施肥来提高放牧者的植被产量。长期研究了长期施肥对植物和微生物群落的影响。然而,尽管短期施肥对QTP中的丛枝菌根真菌(AMF)群落具有重要的作用,但其影响在很大程度上尚不清楚。
    方法:我们使用Illumina MiSeq平台(PE 300)在QTP上的一个实验现场调查了AMF群落对添加N和/或P的三年的响应。
    结果:施肥导致AMF群落组成发生了巨大变化,而NP的添加显着增加了AMF物种的丰富度和系统发育多样性。地上生物量,有效磷和NO3(-)与AMF群落结构的变化显着相关。这些因素的变化是受精处理的驱动。因此,受地上生产力和土壤化学变化的影响,施肥对AMF群落有很大的影响。
    讨论:先前的工作表明植物在施肥后如何经常降低对AMF共生的依赖,从而降低AMF的丰度和多样性。但是,我们的研究报告说,施肥会增加AMF的多样性。由于AMF可以为寄主提供抗逆性,因此我们建议QTP上的极端天气可能有助于推动肥料改良剂与AMF多样性之间的正相关关系。
  • 【传统非转基因大豆品种的矿物成分与氮肥的关系。】 复制标题 收藏 收藏
    DOI:10.1155/2020/9374564 复制DOI
    作者列表:Szostak B,Głowacka A,Klebaniuk R,Kiełtyka-Dadasiewicz A
    BACKGROUND & AIMS: :Soybean is widely used as food. Genetic factors, as well as agrotechnical procedures, affect the yield and quality of soybeans. The subject of our research was the synchronization between soil N supply (from both mineralization and fertilization) and crop N demand. The aim of the research was to determine the effect of the cultivar and nitrogen application on the seed yield and mineral content in soybeans. Two non-GMO soybean cultivars (Amandine and Merlin) and four mineral nitrogen fertilizers ((A) N 0, control; (B) N 30:0, 30 kg N ha-1 before sowing; (C) N 0:30, 30 kg N ha-1 at BBCH 73-75; (D) N 30:30, 30 kg N ha-1 before sowing and 30 kg N ha-1 at BBCH 73-75) were tested. The highest soybean yield was obtained following nitrogen application at a rate of 60 kg ha-1. The genetic factor was found to significantly influence the content of some macronutrients (P, K, and Mg) and micronutrients (Cu, Mn, and Fe). In general, the Merlin cultivar had better macronutrient parameters except nitrogen, while Amandine had a higher content of Cu and Fe. Nitrogen fertilization decreased the content of P, K, and Zn in the soybeans but significantly increased the content of Ca, Mg, Cu, and Mn.
    背景与目标: :大豆被广泛用作食品。遗传因素以及农业技术程序都会影响大豆的产量和品质。我们研究的主题是土壤氮供应(来自矿化和施肥)与作物氮需求之间的同步。该研究的目的是确定栽培品种和施氮量对大豆种子产量和矿物质含量的影响。两个非转基因大豆品种(Amandine和Merlin)和四个矿物氮肥((A)N 0,对照;(B)N 30:0,30 kg N ha-1播种前;(C)N 0:30, (BB)73-75时30kg N ha-1;(D)N 30:30,播前30 kg N ha-1和BBCH 73-75时30 kg N ha-1)。施氮量为60 kg ha-1后,大豆产量最高。发现遗传因素显着影响某些常量营养素(磷,钾和镁)和微量营养素(铜,锰和铁)的含量。通常,除氮外,Merlin品种的常量营养素参数更好,而Amandine的Cu和Fe含量更高。施氮减少了大豆中P,K和Zn的含量,但显着增加了Ca,Mg,Cu和Mn的含量。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录