BACKGROUND & AIMS:
:It is well known that clinically relevant concentrations of iodine-containing radiographic contrast media (CM) induce morphological changes in human erythrocytes. However, there are only few reports about CM effects on erythrocytes of animals (e.g. mice, rats, rabbits, and pigs). Thus, two conventional iodine-containing CM (iodixanol, Visipaque™ 320; iomeprol, Iomeprol™ 350) were tested for their effects on the morphology of erythrocytes from these. After venous blood sampling and blood centrifugation, the autologous plasma was supplemented with 40 vol% CM. Then, a defined number of erythrocytes was incubated in this CM-supplemented plasma for 5 min at body temperature (37°C). Subsequently, 10 μL of the cell suspension were transferred to a purified glass slide and the number of discocytes, echinocytes, and acanthocytes was counted within a total number of 100 erythrocytes (40 fold primary magnification, transmitted light mode). Shape changes of the erythrocytes from all animal species strongly depended on the type of CM and compared to the effects which have already been described for human erythrocytes. Incubation in both CM resulted in morphological changes of the erythrocytes. Incubation in a iodixanol/plasma mixture induced the lowest echinocyte or acanthocyte formation. Porcine erythrocytes showed a much more distinct shape change than those of other animal species and humans. These results suggest erythrocytes from mice, rats, and rabbits are a suitable model system for a model system for human erythrocytes when CM effects on the cellular shape of erythrocytes have to be tested. The distinct deformation of the pig erythrocytes could be due to differences in the pig erythrocyte membrane or the physical and chemical constitution of pig erythrocytes.
背景与目标:
:众所周知,临床上相关浓度的含碘射线造影剂(CM)会诱导人红细胞的形态变化。但是,关于CM对动物(例如小鼠,大鼠,兔和猪)的红细胞的作用的报道很少。因此,测试了两种常规的含碘的CM(碘克沙醇,Visipaque TM 320;碘美普尔,Iomeprol TM 350)对它们的红细胞形态的影响。在静脉血取样和血液离心后,自体血浆中补充了40%(体积)的CM。然后,将一定数量的红细胞在补充了CM的血浆中于体温(37°C)下孵育5分钟。随后,将10μL细胞悬液转移到纯化的载玻片上,并在总数为100的红细胞总数中计数盘状细胞,棘突细胞和棘突细胞的数量(一次放大40倍,透射光模式)。来自所有动物物种的红细胞的形状变化在很大程度上取决于CM的类型,并与已经针对人类红细胞描述的效果进行了比较。两个CM中的温育导致红细胞的形态变化。在碘克沙醇/血浆混合物中温育诱导最低的棘突细胞或棘突细胞形成。猪红细胞的形状变化远比其他动物物种和人类的明显变化。这些结果表明,当必须测试CM对红细胞细胞形状的影响时,来自小鼠,大鼠和兔子的红细胞是适用于人红细胞模型系统的模型系统。猪红细胞的明显变形可能是由于猪红细胞膜的差异或猪红细胞的物理和化学组成所致。