BACKGROUND & AIMS:
:The aim was to compare IgE and IgG4 RAST-inhibition assay (RI), monoclonal antibody ELISA (Mab-ELISA), counter current immuno electrophoresis (CCIE) and histamine release from basophil leukocytes (HR) for allergen quantification with special reference to aeroallergen detection. As components of indoor aeroallergens, cat, dog, and Derm. pter. allergen extracts were selected for the experiments. To evaluate unspecific interference, these allergens were compared mutually and with Cladosporium herbarum. Allergen extracts in varying dilutions were mixed with crushed glass fibre filter materials, eluted, recovered by centrifugation, and allergen concentration quantified by the assays. Equal sensitivity was found for both IgE- and IgG4-RI assaying cat allergen (in the range 5-50 SQ-U/ml) and dog allergen (in the range 10(2)-10(3) SQ-U/ml). The IgG4-RI assaying Derm. pter. was more sensitive (50 SQ-U/ml) than IgE-RI (2*10(3) SQ-U/ml). The ranges of allergen detection limits for the Mab-ELISA were equal for cat and Derm. pter. (10-10(2) SQ-U/ml). The range of allergen detection limits for CCIE, assaying dog were 10(4)-10(5) SQ-U/ml. The ranges of allergen detection limits for HR were equal for cat and Derm. pter. (10-10(2) SQ-U/ml), and 10(2)-10(3) SQ-U/ML for dog. Because of cross-reactivity, a minor degree of interference was observed in the IgE-RI and the HR test for the highest concentration of cat and dog allergens.
背景与目标:
:目的是比较IgE和IgG4 RAST抑制测定(RI),单克隆抗体ELISA(Mab-ELISA),逆流免疫电泳(CCIE)和嗜碱性白细胞(HR)释放的组胺以定量过敏原,并特别参考空气过敏原检测。作为室内空气过敏原的成分,猫,狗和皮肤。 pter。选择过敏原提取物用于实验。为了评估非特异性干扰,将这些变应原相互比较并与桔梗比较。将不同稀释度的过敏原提取物与碎玻璃纤维过滤材料混合,洗脱,离心分离回收,并通过化验定量量化过敏原浓度。发现IgE-和IgG4-RI检测猫过敏原(范围为5-50 SQ-U / ml)和狗过敏原(范围为10(2)-10(3)SQ-U / ml)均具有相同的敏感性。 IgG4-RI测定皮肤。 pter。比IgE-RI(2 * 10(3)SQ-U / ml)更敏感(50 SQ-U / ml)。 Mab-ELISA的过敏原检出限的范围对于猫和真皮均相等。 pter。 (10-10(2)SQ-U / ml)。 CCIE的过敏原检出限范围为10(4)-10(5)SQ-U / ml。猫和真皮的HR过敏原检出限范围是相等的。 pter。 (10-10(2)SQ-U / ml)和10(2)-10(3)SQ-U / ML用于狗。由于交叉反应,在IgE-RI和HR测试中观察到猫和狗过敏原的最高浓度的干扰程度较小。