• 【通过新型电纺丝装置制造的3D双功能化神经导管的排列和随机纳米纤维的受控设计。】 复制标题 收藏 收藏
    DOI:10.1038/srep23761 复制DOI
    作者列表:Kim JI,Hwang TI,Aguilar LE,Park CH,Kim CS
    BACKGROUND & AIMS: :Scaffolds made of aligned nanofibers are favorable for nerve regeneration due to their superior nerve cell attachment and proliferation. However, it is challenging not only to produce a neat mat or a conduit form with aligned nanofibers but also to use these for surgical applications as a nerve guide conduit due to their insufficient mechanical strength. Furthermore, no studies have been reported on the fabrication of aligned nanofibers and randomly-oriented nanofibers on the same mat. In this study, we have successfully produced a mat with both aligned and randomly-oriented nanofibers by using a novel electrospinning set up. A new conduit with a highly-aligned electrospun mat is produced with this modified electrospinning method, and this proposed conduit with favorable features, such as selective permeability, hydrophilicity and nerve growth directional steering, were fabricated as nerve guide conduits (NGCs). The inner surface of the nerve conduit is covered with highly aligned electrospun nanofibers and is able to enhance the proliferation of neural cells. The central part of the tube is double-coated with randomly-oriented nanofibers over the aligned nanofibers, strengthening the weak mechanical strength of the aligned nanofibers.
    背景与目标: :由对齐的纳米纤维制成的脚手架由于其优越的神经细胞附着和增殖能力而有利于神经再生。然而,不仅要产生具有对齐的纳米纤维的整洁的垫子或导管形式,而且由于它们的机械强度不足而将它们用于外科手术作为神经引导导管也是具有挑战性的。此外,还没有关于在同一垫子上制造取向纳米纤维和随机取向的纳米纤维的研究的报道。在这项研究中,我们通过使用新型的静电纺丝技术成功地生产了具有取向和随机取向的纳米纤维的垫子。用这种改进的静电纺丝方法生产了一种具有高度对准的静电纺丝垫的新型导管,并将这种具有良好特性(如选择性渗透性,亲水性和神经生长定向操纵)的拟议导管制成了神经引导导管(NGC)。神经导管的内表面覆盖有高度排列的电纺纳米纤维,能够增强神经细胞的增殖。管的中央部分在取向的纳米纤维上被随机取向的纳米纤维双重涂覆,从而增强了取向的纳米纤维的弱机械强度。
  • 【一种具有通过同轴电纺丝形成的核-壳结构纳米纤维的血管组织工程支架及其生物相容性评价。】 复制标题 收藏 收藏
    DOI:10.1088/1748-6041/11/3/035007 复制DOI
    作者列表:Duan N,Geng X,Ye L,Zhang A,Feng Z,Guo L,Gu Y
    BACKGROUND & AIMS: :In this article, a tubular vascular tissue engineering scaffold with core-shell structured fibers was produced by coaxial electrospinning at an appropriate flow rate ratio between the inner and outer solution. PCL was selected as the core to provide the mechanical property and integrity to the scaffold while collagen was used as the shell to improve the attachment and proliferation of vascular cells due to its excellent biocompatibility. The fine core-shell structured fibers were demonstrated by scanning electron microscope and transmission electron microscope observations. Subsequently, the collagen shell was crosslinked by genipin and further bound with heparin. The crosslinking process was confirmed by the increasing of tensile strength, swelling ratio and thermogravimetric analysis measurements while the surface heparin content was characterized by means of a UV-spectrophotometer and activated partial thromboplastin time tests. Furthermore, the mechanical properties such as stitch strength and bursting pressure of the as-prepared scaffold were measured. Moreover, the biocompatibility of the scaffold was evaluated by cytotoxicity investigation with L929 cells via MTT assay. Endothelial cell adhesion assessments were conducted to reveal the possibility of the formation of an endothelial cell layer on the scaffold surface, while the ability of smooth muscle cell penetration into the scaffold wall was also assessed by confocal laser scanning microscopy. The as-prepared core-shell structured scaffold showed promising potential for use in vascular tissue engineering.
    背景与目标: :在本文中,通过在内部溶液和外部溶液之间的适当流速比下进行同轴电纺丝,生产了具有核-壳结构纤维的管状血管组织工程支架。选择PCL作为核心,以为支架提供机械性能和完整性,而由于其优异的生物相容性,将胶原蛋白用作外壳,以改善血管细胞的附着和增殖。通过扫描电子显微镜和透射电子显微镜观察证明了核壳结构细纤维。随后,胶原蛋白壳通过Genipin交联并进一步与肝素结合。交联过程通过提高抗张强度,溶胀率和热重分析法测定得到证实,而表面肝素含量则通过紫外分光光度计和活化的部分凝血活酶时间测试来表征。此外,测量了所制备的脚手架的机械性能,例如针脚强度和破裂压力。此外,通过MTT测定法对L929细胞进行细胞毒性研究,评价了支架的生物相容性。进行内皮细胞粘附评估以揭示在支架表面上形成内皮细胞层的可能性,同时还通过共聚焦激光扫描显微镜评估了平滑肌细胞渗透到支架壁中的能力。所制备的核-壳结构支架显示出在血管组织工程中使用的有希望的潜力。
  • 【静电纺丝法合成中空氧化铜及其在非酶化双氧水传感器中的应用。】 复制标题 收藏 收藏
    DOI:10.1016/j.colsurfb.2012.03.013 复制DOI
    作者列表:Wang B,Luo L,Ding Y,Zhao D,Zhang Q
    BACKGROUND & AIMS: :Cupric acetate/polyacrylonitrile composite nanofibers were prepared by electrospinning and hollow copper oxide (CuO) particles were produced after subsequent thermal treatment process. The electrospun hollow CuO particles modified carbon paste electrode (CPE) was demonstrated for the first time for nonenzymatic hydrogen peroxide (H(2)O(2)) sensor. The structures and morphologies of hollow CuO particles were characterized by scanning electron microscopy and X-ray diffraction spectrum. The assay performance of the modified sensor to H(2)O(2) was evaluated by cyclic voltammetry and amperometry, revealing high sensitivity (1746.50 μA mM(-1) cm(-2)), low detection limit (0.022 μM) and wide linear response of determination of H(2)O(2) oxidation in the range of 0.05 μM to 1.00 mM.
    背景与目标: :通过静电纺丝制备乙酸铜/聚丙烯腈复合纳米纤维,并在随后的热处理工艺后生产出中空的氧化铜(CuO)颗粒。电纺空心CuO颗粒改性碳糊电极(CPE)首次展示了非酶促过氧化氢(H(2)O(2))传感器。用扫描电子显微镜和X射线衍射光谱对中空CuO颗粒的结构和形貌进行了表征。改进的传感器对H(2)O(2)的测定性能通过循环伏安法和电流分析法进行评估,显示出高灵敏度(1746.50μAmM(-1)cm(-2)),低检测限(0.022μM)和H(2)O(2)氧化测定的宽线性响应,范围为0.05μM至1.00 mM。
  • 【通过电纺丝和热压花相结合制造的用于血管组织工程的聚合物支架。】 复制标题 收藏 收藏
    DOI:10.1088/1748-605X/aa8a81 复制DOI
    作者列表:Yan S,Zhang X,Zhang L,Liu H,Wang X,Li Q
    BACKGROUND & AIMS: :Although tissue engineering has shown great advances in recent years, creating proper mechanical properties and cell growth microenvironments is still challenging. In this study, electrospun nanofibrous membranes were hot embossed to develop three-dimensional hierarchical micro/nanostructures that load and culture human umbilical-vein endothelial cells (HUVECs). The hot-embossed membranes exhibited not only superior mechanical properties (the tensile strength was 7.01 ± 0.18 MPa and the tensile modulus was 166.91 ± 15.54 MPa), but also better cell viability evaluated through a CCK-8 assay and fluorescent dye. The grating and well arrays of the micropatterned fibre mats encouraged the HUVECs to proliferate. Therefore, the approach proposed in this paper-combined electrospinning and hot embossing-has bright prospects in biomedical applications for the use of polymer scaffold in tissue engineering.
    背景与目标: :尽管近年来组织工程学已显示出巨大的进步,但是创建适当的机械性能和细胞生长微环境仍然具有挑战性。在这项研究中,对静电纺丝纳米纤维膜进行热压印,以开发三维层次的微/纳米结构,从而加载和培养人脐静脉内皮细胞(HUVEC)。热压花膜不仅显示出优异的机械性能(拉伸强度为7.01±0.18 MPa,拉伸模量为166.91±15.54 MPa),而且还通过CCK-8分析和荧光染料评估了更好的细胞活力。带有微图案的纤维垫的光栅和孔阵列促进了HUVEC的扩散。因此,本文提出的将静电纺丝和热压花相结合的方法在生物医学应用中在组织工程中使用聚合物支架具有广阔的前景。
  • 【通过同轴静电纺丝将药物封装在可生物降解的超细纤维中。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.30564 复制DOI
    作者列表:Huang ZM,He CL,Yang A,Zhang Y,Han XJ,Yin J,Wu Q
    BACKGROUND & AIMS: :This article describes an electrospinning process to fabricate double-layered ultrafine fibers. A bioabsorbable polymer, Polycaprolactone (PCL), was used as the outer layer or the shell and two medically pure drugs, Resveratrol (RT, a kind of antioxidant) and Gentamycin Sulfate (GS, an antibiotic), were used as the inner layers or the cores. Morphology and microstructure of the ultrafine fibers were characterized by scanning electron microscope (SEM) and transmission electron microscopy (TEM), whereas mechanical performance of them was understood through tensile test. In vitro degradation rates of the nanofibrous membranes were determined by measuring their weight loss when immersed in pH 7.4 phosphate-buffered saline (PBS) mixed with certain amount of Pseudomonas lipase for a maximum of 7 days. The drug release behaviors of the RT and GS were measured using a high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-vis) spectroscopy, respectively. It has been found that the drug solutions without any fiber-forming additive could be encapsulated in the PCL ultrafine fibers, although they alone cannot be made into a fiber form. Beads on the fiber surface influenced the tensile behavior of the ultrafine fibers remarkably. When the core solvent was miscible with the shell solvent, higher drug concentration decreased the bead formation and thus favored the mechanical performance. The situation, however, became different if the two solvents were immiscible with each other. The degradation rate was closely related to hydrophilicity of the drugs in the cores. Higher hydrophilicity apparently led to faster degradation. The release profiles of the RT and GS exhibited a sustained release characteristic, with no burst release phenomenon.
    背景与目标: :本文介绍了静电纺丝工艺以制造双层超细纤维。外层或外壳使用可生物吸收的聚合物聚己内酯(PCL),内层使用两种医用纯药物白藜芦醇(RT,一种抗氧化剂)和硫酸庆大霉素(GS)。核心。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)表征了超细纤维的形貌和微观结构,而通过拉伸试验可以了解它们的机械性能。通过将纳米纤维膜浸入与一定量的假单胞菌脂肪酶混合的pH 7.4磷酸盐缓冲盐水(PBS)中,最长不超过7天的重量损失来确定其体外降解速率。 RT和GS的药物释放行为分别使用高效液相色谱(HPLC)和紫外可见光谱(UV-vis)进行了测量。已经发现,没有任何成纤维添加剂的药物溶液可以封装在PCL超细纤维中,尽管不能单独将它们制成纤维形式。纤维表面的珠子显着影响超细纤维的拉伸性能。当核溶剂与壳溶剂混溶时,较高的药物浓度会减少珠粒的形成,从而有利于机械性能。但是,如果两种溶剂互不相溶,情况就会变得不同。降解速率与药芯中的亲水性密切相关。较高的亲水性显然导致更快的降解。 RT和GS的释放曲线表现出持续释放特性,没有爆发释放现象。
  • 【通过乳液静电纺丝将桔红素包裹在PVA / PAA交联的电纺纤维中:形态表征,缓释和抗氧化活性评估。】 复制标题 收藏 收藏
    DOI:10.1016/j.foodchem.2020.127763 复制DOI
    作者列表:Zhan F,Yan X,Li J,Sheng F,Li B
    BACKGROUND & AIMS: :In this work, water-resistant poly (vinyl alcohol) (PVA)/poly (acrylic acid) (PAA) electrospun fibers encapsulating tangeretin (Tan) were fabricated by emulsion-electrospinning. To optimize the electrospinning condition, the size and morphology of electrospun fibers were characterized by dynamic light scattering (DLS), optical light microscope, fluorescence microscopy, and scanning electron microscopy (SEM), respectively. The optimized initial concentration of PVA/PAA was 10% (w/w) with a mass ratio of 3:7. The time and temperature of optimized thermal crosslinking treatment were 2 h and 145 °C, respectively. The results of XRD and SEM showed that the Tan was successfully incorporated into smooth PVA/PAA electrospun fibers and those fibers possessed nano-diameter size and high porosity. The encapsulation of Tan had no significant impact on the antioxidant activity of PVA/PAA/Tan crosslinking electrospun fibers. The in vitro release test showed the PVA/PAA/Tan crosslinking electrospun fibers achieved durable release profiles and lower burst release rates than that from the pure Tan emulsion. Based on these results, it is concluded that PVA/PAA/Tan crosslinking electrospun fibers prepared by emulsion-electrospinning serve as a promising technique in the fields of water-insoluble drug delivery and slow-release.
    背景与目标: :在这项工作中,通过乳液静电纺丝制备了包裹橘皮素(Tan)的耐水聚乙烯醇(PVA)/聚丙烯酸(PAA)电纺纤维。为了优化电纺条件,分别通过动态光散射(DLS),光学显微镜,荧光显微镜和扫描电子显微镜(SEM)对电纺纤维的尺寸和形态进行了表征。 PVA / PAA的最佳初始浓度为10%(w / w),质量比为3:7。优化的热交联处理的时间和温度分别为2 h和145°C。 XRD和SEM的结果表明,Tan成功地掺入了光滑的PVA / PAA电纺纤维中,并且这些纤维具有纳米直径的尺寸和高孔隙率。 Tan的包封对PVA / PAA / Tan交联电纺纤维的抗氧化活性没有明显影响。体外释放测试表明,与纯棕褐色乳液相比,PVA / PAA / Tan交联电纺纤维具有持久的释放特性和较低的破裂释放速率。基于这些结果,得出结论,通过乳液电纺丝制备的PVA / PAA / Tan交联电纺纤维在水不溶性药物递送和缓释领域中是有前途的技术。
  • 【牛血清白蛋白的静电纺丝。优化和用于生产生物传感器。】 复制标题 收藏 收藏
    DOI:10.1021/bm800421s 复制DOI
    作者列表:Kowalczyk T,Nowicka A,Elbaum D,Kowalewski TA
    BACKGROUND & AIMS: :Electrospinning of the globular protein, bovine serum albumin (BSA), was optimized to obtain proteinous fibers suitable as biosensors. It was shown that the as-spun protein preserves its native form, whereas solubility of the cross-linked in the ambient conditions BSA nanofibers evidently decreases. Insoluble BSA fibers can be easily modified to be used as two-dimensional biosensors. Here, we show the micro pH sensor obtained from the BSA fiber stained with a fluorescein derivative (FITC).
    背景与目标: :优化球状蛋白牛血清白蛋白(BSA)的静电纺丝以获得适合作为生物传感器的蛋白纤维。结果表明,初生蛋白保留了其天然形式,而在环境条件下交联的BSA纳米纤维的溶解度则明显降低。不溶性BSA纤维可以很容易地改性以用作二维生物传感器。在这里,我们显示了从BSA纤维获得的微型pH传感器,该纤维被荧光素衍生物(FITC)染色。
  • 【通过电纺丝法制备的β-环糊精/槲皮素包合物纳米膜的表征和抑菌作用。】 复制标题 收藏 收藏
    DOI:10.1016/j.foodchem.2020.127980 复制DOI
    作者列表:Wang Z,Zou W,Liu L,Wang M,Li F,Shen W
    BACKGROUND & AIMS: :Quercetin has various biological activities, but its poor water solubility and stability limit its applications. In this study, β-cyclodextrin was used as the host and quercetin was encapsulated in its cavity to prepare an inclusion compound. Then, a nanofilm was formed using electrospinning. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermogravimetric differential scanning calorimetry (TG-DSC) were used to characterize the properties of the inclusion compound nanofilms. SEM images showed that the nanofilm prepared by optimizing the electrospinning process parameters had a good nanofiber structure. XRD, FTIR and TG/DSC characterization of the nanofilm showed that quercetin was encapsulated in the cavity of β-cyclodextrin and was present in the nanofilm. The quercetin was slowly released from the nanofilm and still had good bacteriostatic effects on Staphylococcus aureus and Escherichia coli, indicating that the process of embedding and electrospinning did not affect the antibacterial activity of quercetin.
    背景与目标: :槲皮素具有多种生物活性,但水溶性和稳定性差,限制了其应用。在这项研究中,β-环糊精被用作宿主,槲皮素被包裹在其腔中以制备包合物。然后,使用静电纺丝形成纳米膜。傅里叶变换红外光谱(FT-IR),X射线衍射(XRD),扫描电子显微镜(SEM)和热重差示扫描量热法(TG-DSC)用于表征包合物纳米薄膜的性能。 SEM图像表明,通过优化静电纺丝工艺参数制备的纳米膜具有良好的纳米纤维结构。纳米膜的XRD,FTIR和TG / DSC表征表明槲皮素被包裹在β-环糊精的空腔中,并存在于纳米膜中。槲皮素从纳米膜上缓慢释放,对金黄色葡萄球菌和大肠杆菌仍具有良好的抑菌作用,表明包埋和静电纺丝过程不影响槲皮素的抗菌活性。
  • 【喷雾干燥,电吹法和静电纺丝法制备Eudragit E和伊曲康唑固体分散体的比较。】 复制标题 收藏 收藏
    DOI:10.1016/j.ijpharm.2015.07.076 复制DOI
    作者列表:Sóti PL,Bocz K,Pataki H,Eke Z,Farkas A,Verreck G,Kiss É,Fekete P,Vigh T,Wagner I,Nagy ZK,Marosi G
    BACKGROUND & AIMS: :Three solvent based methods: spray drying (SD), electrospinning (ES) and air-assisted electrospinning (electroblowing; EB) were used to prepare solid dispersions of itraconazole and Eudragit E. Samples with the same API/polymer ratios were prepared in order to make the three technologies comparable. The structure and morphology of solid dispersions were identified by scanning electron microscopy and solid phase analytical methods such as, X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC) and Raman chemical mapping. Moreover, the residual organic solvents of the solid products were determined by static headspace-gas chromatography/mass spectroscopy measurements and the wettability of samples was characterized by contact angle measurement. The pharmaceutical performance of the three dispersion type, evaluated by dissolution tests, proved to be very similar. According to XRPD and DSC analyses, made after the production, all the solid dispersions were free of any API crystal clusters but about 10 wt% drug crystallinity was observed after three months of storage in the case of the SD samples in contrast to the samples produced by ES and EB in which the polymer matrix preserved the API in amorphous state.
    背景与目标: :三种基于溶剂的方法:喷雾干燥(SD),电纺(ES)和空气辅助电纺(电吹; EB)用于制备伊曲康唑和Eudragit E的固体分散体。按顺序制备具有相同API /聚合物比的样品使这三种技术具有可比性。通过扫描电子显微镜和固相分析方法,如X射线粉末衍射(XRPD),差示扫描量热法(DSC)和拉曼化学作图,鉴定了固体分散体的结构和形态。此外,通过静态顶空-气相色谱/质谱法测定来测定固体产物的残留有机溶剂,并通过接触角测定来表征样品的润湿性。通过溶出度测试评估的三种分散体类型的药物性能非常相似。根据生产后进行的XRPD和DSC分析,所有固体分散体均不含任何API晶体簇,但与生产的样品相比,SD样品在储存三个月后观察到约10 wt%的药物结晶度通过ES和EB,其中聚合物基质将API保持在非晶态。
  • 【低分子量可交联大分子单体对聚丙交酯-乙交酯共聚物纤维电纺丝的影响。】 复制标题 收藏 收藏
    DOI:10.1163/156856207782246821 复制DOI
    作者列表:Xu W,He X,Sarvestani AS,Jabbari E
    BACKGROUND & AIMS: :A mixture of low-molecular-weight poly(L-lactide-co-glycolide ethylene oxide fumarate) (PLGEOF) macromer and high-molecular-weight poly(lactide-co-glycolide) (PLGA) was used to produce fibers by electrospinning. PLGEOF is a biodegradable and in situ cross-linkable terpolymer made from building blocks with excellent biocompatibility. PLGA provides the required elongational viscosity to the spinning jet while the unsaturated PLGEOF macromers contribute to in situ crosslinking of fibers and attachment of bioactive functional groups. Mechanical rheometry demonstrated that PLGEOF macromers cross-link in situ by ultraviolet radiation. The addition of PLGEOF macromer to PLGA solutions had a significant effect on size and morphology of the electrospun fibers. The morphology of the electrospun fibers changed from bead- to fiber-like with increasing PLGEOF concentration. As PLGEOF was added to 12 wt% PLGA solutions, the fiber diameter first decreased with 2% PLGEOF and then increased with the addition of 5% and 10% PLGEOF. Our results demonstrate that the fiber size initially is decreased with the addition of PLGEOF due to an increase in solution conductivity and then is increased with further PLGEOF addition due to higher viscosity of the polymerizing mixture.
    背景与目标: :使用低分子量聚(L-丙交酯-乙交酯-环氧乙烷富马酸酯)(PLGEOF)大分子单体和高分子量聚(丙交酯-乙交酯)(PLGA)的混合物通过静电纺丝生产纤维。 PLGEOF是由具有出色生物相容性的结构单元制成的可生物降解的原位可交联三元共聚物。 PLGA向纺丝喷嘴提供所需的伸长粘度,而不饱和PLGEOF大分子单体则有助于纤维的原位交联和生物活性官能团的附着。机械流变学证明PLGEOF大分子单体通过紫外线辐射原位交联。将PLGEOF大分子单体添加到PLGA溶液中对电纺纤维的尺寸和形态有重大影响。随着PLGEOF浓度的增加,电纺纤维的形态从珠状变为类纤维。当将PLGEOF添加到12 wt%PLGA溶液中时,纤维直径首先在2%PLGEOF下减小,然后在添加5%和10%PLGEOF时增大。我们的结果表明,由于溶液电导率的增加,纤维尺寸最初随着添加PLGEOF而减小,然后由于聚合混合物的较高粘度而随着进一步PLGEOF添加而增大。
  • 【3D耳廓形支架的直接静电纺丝,用于组织工程应用。】 复制标题 收藏 收藏
    DOI:10.1088/1758-5090/8/2/025007 复制DOI
    作者列表:Walser J,Stok KS,Caversaccio MD,Ferguson SJ
    BACKGROUND & AIMS: :Thirty-two poly(ε)caprolactone (PCL) scaffolds have been produced by electrospinning directly into an auricle-shaped mould and seeded with articular chondrocytes harvested from bovine ankle joints. After seeding, the auricle shaped constructs were cultured in vitro and analysed at days 1, 7, 14 and 21 for regional differences in total DNA, glycosaminoglycan (GAG) and collagen (COL) content as well as the expression of aggrecan (AGG), collagen type I and type II (COL1/2) and matrix metalloproteinase 3 and 13 (MMP3/13). Stress-relaxation indentation testing was performed to investigate regional mechanical properties of the electrospun constructs. Electrospinning into a conductive mould yielded stable 3D constructs both initially and for the whole in vitro culture period, with an equilibrium modulus in the MPa range. Rapid cell proliferation and COL accumulation was observed until week 3. Quantitative real time PCR analysis showed an initial increase in AGG, no change in COL2, a persistent increase in COL1, and only a slight decrease initially for MMP3. Electrospinning of fibrous scaffolds directly into an auricle-shape represents a promising option for auricular tissue engineering, as it can reduce the steps needed to achieve an implantable structure.
    背景与目标: :已经通过直接电纺入耳廓形模具并植入从牛踝关节收获的关节软骨细胞来生产32个聚(ε)己内酯(PCL)支架。播种后,将耳廓形构建体进行体外培养,并在第1、7、14和21天分析总DNA,糖胺聚糖(GAG)和胶原蛋白(COL)含量以及聚集蛋白聚糖(AGG)的表达的区域差异, I型和II型胶原(COL1 / 2)以及基质金属蛋白酶3和13(MMP3 / 13)。进行应力松弛压痕测试以研究电纺结构的区域机械性能。静电纺成导电模具可在最初和整个体外培养期间产生稳定的3D结构,其平衡模量在MPa范围内。观察到快速的细胞增殖和COL积累,直到第3周。定量实时PCR分析显示,AGG最初增加,COL2没有变化,COL1持续增加,而MMP3最初仅略有下降。将纤维支架直接电纺成耳廓形状代表了耳廓组织工程的一个有前途的选择,因为它可以减少实现可植入结构所需的步骤。
  • 【牺牲纤维的双重静电纺丝,可改善工程孔隙率并增强组织向内生长。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.b.33695 复制DOI
    作者列表:Voorneveld J,Oosthuysen A,Franz T,Zilla P,Bezuidenhout D
    BACKGROUND & AIMS: :Porosity, pore size and pore interconnectivity are critical factors for cellular infiltration into electrospun scaffolds. This study utilized dual electrospinning with sacrificial fiber extraction to produce scaffolds with engineered porosity and mechanical properties. Subsequently, scaffolds were covalently grafted with heparin, a known anti-coagulant with growth-factor binding properties. We hypothesized that the tissue ingrowth would correlate positively with the porosity of the scaffolds. Pellethane® (PU) was spun simultaneously with poly(ethylene oxide) (PEO, subsequently extracted). Low, medium and high porosity scaffolds and heparinized versions of each were characterized and implanted in vivo for evaluation of cellular infiltration and inflammation subcutaneously in male Wistar rats (7,14 and 28 days, n = 6). Average pore-size for low (76 ± 0.2%), medium (83 ± 0.5%) and high (90 ± 1.0%) porosity scaffolds was 4.0 ± 2.3 µm, 9.9 ± 4.2 µm and 11.1 ± 5.5 µm (p < 0.0001). Heparinization resulted in increased fiber diameter (3.6 ± 1.1 µm vs. 1.8 ± 0.8 µm, p < 0.0001) but influenced neither pore-size (p = 0.67) nor porosity (p = 0.27). Cellular infiltration for low, medium and high porosity scaffolds reached 33 ± 7%, 77 ± 20% and 98 ± 1% of scaffold width, respectively, by day 28 of implantation (p < 0001); heparinization did not affect infiltration (p = 0.89). The ultimate tensile strength (UTS) and Young's modulus (Ey ) of the constructs increased linearly with increasing PU fiber fraction (UTS: r2  = 0.97, p < 0.0001, Ey : r2 = 0.76, p < 0.0001) and heparinization resulted in decreased strength but increased stiffness compared to non-heparinized scaffolds. Increased PEO to PU fraction in the scaffold resulted in predictable losses to mechanical strength and improvements to cellular infiltration, which could make PEO to PU fraction a useful optimization parameter for small diameter vascular grafts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1559-1572, 2017.
    背景与目标: :孔隙度,孔径和孔隙互连性是细胞渗透到电纺支架中的关键因素。这项研究利用双重电纺与牺牲纤维的提取来生产具有工程孔隙率和机械性能的支架。随后,将支架与肝素共价移植,肝素是一种具有生长因子结合特性的已知抗凝剂。我们假设组织向内生长将与支架的孔隙率成正相关。 (PU)与聚环氧乙烷(PEO,随后萃取)同时纺丝。对低,中,高孔隙率的支架和肝素化的每种支架进行表征并植入体内,以评估雄性Wistar大鼠皮下的细胞浸润和炎症(7、14和28天,n = 6)。低(76±0.2%),中(83±0.5%)和高(90±1.0%)多孔支架的平均孔径为4.0±2.3 µm,9.9±4.2 µm和11.1±5.5 µm(p <0.0001) 。肝素化导致纤维直径增加(3.6±1.1 µm与1.8±0.8 µm,p <0.0001),但既不影响孔径(p = 0.67),也不影响孔隙率(p = 0.27)。到植入第28天时,低,中和高孔隙率支架的细胞浸润分别达到支架宽度的33%±7%,77%±20%和98%±1%(p <0001);肝素化不影响浸润(p = 0.89)。结构的极限抗拉强度(UTS)和杨氏模量(Ey)随着PU纤维含量的增加而线性增加(UTS:r2 = 0.97,p <0.0001,Ey:r2 = 0.76,p <0.0001),肝素化导致强度降低但与未肝素化的支架相比增加了刚度。支架中PEO占PU分数的增加导致机械强度的可预测损失和细胞浸润的改善,这可能使PEO占PU分数成为用于小直径血管移植物的有用的优化参数。 ©2016 Wiley Periodicals,Inc.J Biomed Mater Res B部分:Appl Biomater,105B:1559-1572,2017。
  • 【通过双源双功率静电纺丝制成的双组分纤维支架:rhBMP-2和Ca-P纳米颗粒的双重递送并增强了生物学性能。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.36084 复制DOI
    作者列表:Wang C,Lu WW,Wang M
    BACKGROUND & AIMS: :Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning. The release behavior of rhBMP-2 and Ca2+ ions could be separately tuned and the released rhBMP-2 retained a 68% level for biological activity. MC3T3-E1 cells showed high viability and normal morphology on scaffolds. Compared to monocomponent scaffolds, enhanced cell proliferation, alkaline phosphatase activity, cell mineralization, and gene expression of osteogenic markers were achieved for bicomponent scaffolds due to the synergistic effect of rhBMP-2 and Ca-P nanoparticles. Bicomponent scaffolds with a double mass elicited further enhanced cell adhesion, spreading, proliferation, and osteogenic differentiation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2199-2209, 2017.
    背景与目标: :结合磷酸钙(Ca-P)和骨形态发生蛋白2(BMP-2)的电纺支架已用于骨骼组织再生。但是,在大多数情况下,BMP-2和Ca-P只是简单地混合并以整体结构装载,存在BMP-2装载水平低,BMP-2生物活性降低,BMP-2释放不受控制以及Ca-P分布不均匀的风险。在这项研究中,利用乳液电纺和共混电纺的成熟双源双功率电纺技术,制备了具有均匀分布的含rhBMP-2纤维和含Ca-P纳米粒子的纤维的新型双组分支架。可以分别调节rhBMP-2和Ca2离子的释放行为,释放的rhBMP-2保留68%的生物活性。 MC3T3-E1细胞在支架上显示出高活力和正常形态。与单组分支架相比,由于rhBMP-2和Ca-P纳米颗粒的协同作用,双组分支架实现了增强的细胞增殖,碱性磷酸酶活性,细胞矿化和成骨标记基因表达。具有双重质量的双组分支架引发了进一步增强的细胞粘附,扩散,增殖和成骨分化。分级为4 +©2017 Wiley Periodicals,Inc.J Biomed Mater Res Part A:105A:2199-2209,2017。
  • 【新型可生物降解的聚(酯氨基甲酸酯)和聚(酯氨基甲酸酯尿素)的静电纺丝,用于软组织工程应用。】 复制标题 收藏 收藏
    DOI:10.1007/s10856-009-3768-3 复制DOI
    作者列表:Caracciolo PC,Thomas V,Vohra YK,Buffa F,Abraham GA
    BACKGROUND & AIMS: :The development of biomimetic highly-porous scaffolds is essential for successful tissue engineering. Segmented poly(ester urethane)s and poly(ester urethane urea)s have been infrequently used for the fabrication of electrospun nanofibrous tissues, which is surprising because these polymers represent a very large variety of materials with tailored properties. This study reports the preparation of new electrospun elastomeric polyurethane scaffolds. Two novel segmented polyurethanes (SPU), synthesized from poly(epsilon-caprolactone) diol, 1,6-hexamethylene diisocyanate, and diester-diphenol or diurea-diol chain extenders, were used (Caracciolo et al. in J Mater Sci Mater Med 20:145-155, 2009). The spinnability and the morphology of the electrospun SPU scaffolds were investigated and discussed. The electrospinning parameters such as solution properties (polymer concentration and solvent) and processing parameters (applied electric field, needle to collector distance and solution flow rate) were optimized to achieve smooth, uniform bead-free fibers with diameter (~700 nm) mimicking the protein fibers of native extracellular matrix (ECM). The obtained elastomeric polyurethane scaffolds could be appropriate for soft tissue-engineering applications.
    背景与目标: :仿生高多孔性支架的开发对于成功的组织工程至关重要。分段聚(酯氨基甲酸酯)和聚(酯氨基甲酸酯脲)已经很少用于电纺纳米纤维组织的制造,这是令人惊讶的,因为这些聚合物代表了具有定制性质的多种材料。这项研究报告了新型电纺弹性聚氨酯支架的制备。使用了两种由聚(ε-己内酯)二醇,1,6-六亚甲基二异氰酸酯和二酯-二酚或二脲-二醇扩链剂合成的新型分段聚氨酯(SPU)(Caracciolo et al。in J Mater Sci Mater Med 20 :145-155,2009)。研究并讨论了静电纺SPU支架的可纺性和形态。优化了电纺丝参数,例如溶液特性(聚合物浓度和溶剂)和加工参数(施加的电场,针到收集器的距离和溶液流速),以实现直径均匀(〜700 nm)的光滑,均匀的无珠纤维,其模拟天然细胞外基质(ECM)的蛋白质纤维。所获得的弹性体聚氨酯支架可适用于软组织工程应用。
  • 【聚(乙烯-共-乙烯醇)纤维的静电纺丝。】 复制标题 收藏 收藏
    DOI:10.1016/s0142-9612(02)00422-2 复制DOI
    作者列表:Kenawy el R,Layman JM,Watkins JR,Bowlin GL,Matthews JA,Simpson DG,Wnek GE
    BACKGROUND & AIMS: :Solutions of poly(ethylene-co-vinyl alcohol) or EVOH, ranging in composition from 56 to 71 wt% vinyl alcohol, can be readily electrospun at room temperature from solutions in 70% 2-propanol/water (rubbing alcohol). The solutions are prepared at 80 degrees C and allowed to cool to room temperature. Interestingly, the solutions are not stable at room temperature and eventually the polymer precipitates after several hours. However, prior to precipitation, electrospinning is extensive and rapid, allowing coverage of fibers on various substrates, including a grounded metal plate, dielectrics interposed between the charged jet and the metal ground, and on the human body. Fiber diameters of ca. 0.2-8.0 microm were obtained depending upon the solution concentration, an attractive range for tissue engineering, wound healing, and related applications. Electrospun EVOH mats have been shown to support the culturing of smooth muscle cells and fibroblasts.
    背景与目标: :聚乙烯(乙烯-共-乙烯醇)或EVOH溶液的组成范围为56至71 wt%的乙烯醇,在室温下可以很容易地从70%2-丙醇/水(摩擦醇)溶液中进行电纺丝。在80℃下制备溶液,并使其冷却至室温。有趣的是,溶液在室温下不稳定,最终聚合物在数小时后沉淀。然而,在沉淀之前,静电纺丝是广泛且快速的,从而允许将纤维覆盖在各种基底上,包括接地的金属板,介于带电射流和金属基底之间以及人体上的电介质。纤维直径约根据溶液浓度,组织工程,伤口愈合和相关应用的吸引范围而获得0.2-8.0微米。静电纺丝EVOH垫已被证明可以支持平滑肌细胞和成纤维细胞的培养。

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