• 【一种固态多西环素HP-β-CD制剂,可通过按比例放大的静电纺丝法制备(静脉推注)。】 复制标题 收藏 收藏
    DOI:10.1016/j.ijpharm.2020.119539 复制DOI
    作者列表:Kiss K,Vass P,Farkas A,Hirsch E,Szabó E,Mező G,Nagy ZK,Marosi G
    BACKGROUND & AIMS: :In this study a new intravenous (i.v.) bolus dosage form of doxycycline was prepared by electrospinning. A tetracycline-type antibiotic with low water solubility (doxycycline (DOX)) was used with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) as solubilizer. The new solid formulation could be produced with high (~80 g/h) productivity rate using high-speed electrospinning (HSES) from a water-based precursor solution. Freeze-dried DOX-HP-β-CD was also prepared from the same precursor solution as HSES for comparison. Raman mapping showed that the amorphous DOX was uniformly distributed in the fibrous powder making precise dosing of the API possible. The new formulation's viability as an i.v. bolus product was examined with reconstitution test. The samples contained 100 mg of pure DOX (similarly to the products currently on the market). To ensure i.v. bolus applicability, the dissolution was carried out in 1.5 mL water. The final DOX concentration was 66.7 mg/(mL solvent), which is 7 times higher than the currently marketed formulation. The drug release was followed by UV-VIS spectrophotometry. The results confirmed that the reconstitution solution could be applied as an i.v. bolus dosage form. Moreover, the work confirmed that the continuous high-speed electrospinning process can be a viable high productivity alternative to freeze-drying.
    背景与目标: :在这项研究中,通过静电纺丝制备了一种新的多西环素静脉内(静脉)推注剂型。将水溶性低的四环素类抗生素(强力霉素(DOX))与2-羟丙基-β-环糊精(HP-β-CD)一起用作增溶剂。新的固体配方可以使用高速前纺(HSES)从水基前体溶液中以高(〜80 g / h)的生产率生产。还从与HSES相同的前体溶液中制备了冻干的DOX-HP-β-CD,用于比较。拉曼作图表明,无定形的DOX均匀地分布在纤维粉末中,从而使API的精确定量成为可能。新配方的i.v.推注产品经重构试验检查。样品中含有100 mg的纯DOX(类似于当前市场上的产品)。为了确保i.v.推注适用性,在1.5 mL水中溶解。最终的DOX浓度为66.7 mg /(mL溶剂),比目前市场上出售的制剂高7倍。药物释放后用UV-VIS分光光度法测定。结果证实,该重构溶液可以作为静脉注射应用。推注剂型。此外,这项工作证实,连续高速静电纺丝工艺可以替代冷冻干燥,是一种可行的高生产率方法。
  • 【具有多层多孔结构的布状碳纳米纤维薄膜的静电纺丝工艺及其在深度脱硫中的应用。】 复制标题 收藏 收藏
    DOI:10.1016/j.ecoenv.2020.110555 复制DOI
    作者列表:Sun XL,Liu Z,Cheng ZL
    BACKGROUND & AIMS: :A strategy for clean fuel by selective adsorption processing was deemed to be convenient and environmental-friendly in past decades. However, the development of adsorption desulfurization was tremendously subject to the fabrication of high-performance adsorbents with large capacity and high stability. Herein, we designed a novel route to fabricate the cloth-like carbon nanofiber film with a hierarchical porous structure by electrospinning. The structure and properties of the cloth-like carbon nanofiber films were determined by a series of characterizations. Subsequently, the desulfurization performance of the cloth-like carbon nanofiber films was examined by the simulated thiophene (TH) oil. Furthermore, the effect of adsorption conditions on the adsorption capacity was intensively investigated, such as carbonization temperature, initial concentration and desulfurization temperature. The results found that at optimal calcination temperature of 700 °C, the cloth-like carbon nanofiber films possessed the highest micropore volume (Vmic = 0.185 m3/g) and adsorption capacity (qe = 96.6 mg/g) at 800 mg/L initial concentration under the adsorption temperature of 25 °C. The results corroborated that the physical properties of the cloth-like carbon nanofiber films with the surface area of 417.8 m2/g, the total pore volume of 0.187 cm3/g and average pore diameter of 1.36 nm had an important influence on the high adsorption capacity. On this basis, the adsorption experimental data were best fitted to pseudo-second-order kinetic and Langmuir isotherm models. Furthermore, the other highlight of the cloth-like carbon nanofiber films was convenient for the separation from oil, thus achieving the desirable reused performance.
    背景与目标: :在过去的几十年中,通过选择性吸附处理获得清洁燃料的策略被认为是方便且环保的。然而,吸附脱硫的发展极大地受到了制备具有大容量和高稳定性的高性能吸附剂的影响。在这里,我们设计了一种新颖的方法,通过静电纺丝来制造具有分层多孔结构的布状碳纳米纤维薄膜。布状碳纳米纤维薄膜的结构和性能是通过一系列表征确定的。随后,通过模拟的噻吩(TH)油检查了布状碳纳米纤维薄膜的脱硫性能。此外,深入研究了吸附条件对吸附容量的影响,如碳化温度,初始浓度和脱硫温度。结果发现,在700°C的最佳煅烧温度下,布状碳纳米纤维薄膜在800 mg / L的初始浓度下具有最高的微孔体积(Vmic = 0.185 m3 / g)和吸附容量(qe = 96.6 mg / g)吸附温度为25°C时的浓度。结果证实了表面积为417.8×m2 / g,总孔体积为0.187×cm3 / g,平均孔径为1.36nm的布状碳纳米纤维薄膜的物理性能对高吸附能力有重要影响。在此基础上,吸附实验数据最适合拟二阶动力学模型和Langmuir等温模型。此外,布状碳纳米纤维膜的另一个亮点是便于从油中分离,从而实现了理想的重复使用性能。
  • 【用于药物递送的静电纺丝的最新发展。】 复制标题 收藏 收藏
    DOI:10.3390/pharmaceutics12010005 复制DOI
    作者列表:Zelkó R,Lamprou DA,Sebe I
    BACKGROUND & AIMS: :Electrospinning is one of the most widely used techniques for the fabrication of nano/microparticles and nano/microfibers, induced by a high voltage applied to the drug-loaded solution [...].
    背景与目标: 静电纺丝是通过施加到载药溶液上的高电压诱导的制造纳米/微粒和纳米/微纤维的最广泛使用的技术之一。
  • 【通过电纺丝制备的葡聚糖膜的优化和表征。】 复制标题 收藏 收藏
    DOI:10.1021/bm034345w 复制DOI
    作者列表:Jiang H,Fang D,Hsiao BS,Chu B,Chen W
    BACKGROUND & AIMS: :Dextran is soluble in both water and organic solvents, so it could be a versatile biomacromolecule for preparing nanofibrous electrospun membranes by blending with either water-soluble bioactive agents or hydrophobic biodegradable polymers for biomedical applications. We have formulated electrospun dextran membranes, and the effects of various processing parameters on the membrane properties were investigated. It was found that uniform nanofibrous dextran membranes could be formed by using water, DMSO/water, and DMSO/DMF mixtures as solvents through adjusting the processing conditions (solution concentration, voltage, and the distance between the electrode and the collecting plate). When water was used as a solvent, up to 10% (w/w) of bovine serum albumin (BSA) or lysozyme could be directly incorporated into the dextran electrospun membrane without compromising its morphology. No significant effect of the electrospinning process on lysozyme activity was observed. The composite electrospun membranes consisting of poly(D,L-lactide-co-glycolide) (PLGA) and dextran were obtained using DMSO/DMF (50/50, volume ratio) mixture as solvents. For cross-linking the electrospun membrane, dextran was modified by substitution of methacrylate groups at the hydroxyl sites. It was found that the electrospun membranes prepared from methacrylated dextran can be cured by UV irradiation in the presence of 1% of 2,2-dimethoxy-2-phenylacetophenone (DMPA) as a photoinitiator.
    背景与目标: :Dextran可溶于水和有机溶剂,因此通过与水溶性生物活性剂或疏水性可生物降解的聚合物共混用于生物医学应用,它可以成为制备纳米纤维电纺膜的多功能生物大分子。我们配制了静电纺丝的右旋糖酐膜,并研究了各种工艺参数对膜性能的影响。已经发现,通过调节处理条件(溶液浓度,电压以及电极与集电板之间的距离),以水,DMSO /水和DMSO / DMF混合物为溶剂可以形成均匀的纳米纤维葡聚糖膜。当使用水作为溶剂时,可以将不超过10%(w / w)的牛血清白蛋白(BSA)或溶菌酶直接掺入葡聚糖电纺膜中,而不会影响其形态。没有观察到静电纺丝过程对溶菌酶活性的显着影响。使用DMSO / DMF(50/50,体积比)混合物作为溶剂,获得由聚(D,L-丙交酯-共-乙交酯)(PLGA)和右旋糖酐组成的复合电纺膜。为了使电纺膜交联,通过在羟基位点取代甲基丙烯酸酯基团对葡聚糖进行改性。发现由甲基丙烯酸酯化的葡聚糖制备的电纺膜可以在1%的2,2-二甲氧基-2-苯基苯乙酮(DMPA)作为光引发剂存在下通过UV辐射固化。
  • 【冷冻凝胶法制备的双层水凝胶电纺丝支架的制备和评价。】 复制标题 收藏 收藏
    DOI:10.1016/j.jbiomech.2019.109466 复制DOI
    作者列表:Kamali A,Shamloo A
    BACKGROUND & AIMS: :This study presents a bilayer structure as a skin scaffold comprised of an electrospun sheet layer made of polycaprolactone and polyvinil alcohol and a porous hydrogel layer made of chitosan and gelatin. The hydrogel layer was fabricated by employing the freeze-gelation technique. The bilayer structure was achieved by pouring the hydrogel solution on the electrospun sheet at the bottom of a mold followed by the freeze-gelation technique to obtain a porous structure in the hydrogel. The hydrogel and hydrogel-electrospun samples were characterized by scanning electron microscopy, swelling, tensile strength, in vitro and in vivo analyses. From a mechanical strength standpoint, the combination of hydrogel and electrospun layers produced 110 and 133% increases in mean tensile strength and elastic modulus values, respectively compared to the single layer hydrogel sample. While the results of swelling and cell proliferation tests did not yield significant differences between the two groups, the bilayer scaffold performed better considering the results of the in vivo analysis. It was also seen that there was a good connection between the two layers in the bilayer scaffold, making the necessary manipulations and clinical handling convenient.
    背景与目标: :这项研究提出了一种双层结构,作为皮肤支架,由聚己内酯和聚乙烯醇制成的电纺片层和壳聚糖和明胶制成的多孔水凝胶层组成。水凝胶层是通过采用冷冻凝胶技术制备的。通过将水凝胶溶液倒在模具底部的电纺片上,然后进行冷冻凝胶化技术以获得水凝胶中的多孔结构,从而获得双层结构。通过扫描电子显微镜,溶胀,拉伸强度,体外和体内分析来表征水凝胶和水凝胶电纺丝样品。从机械强度的观点来看,与单层水凝胶样品相比,水凝胶层和电纺丝层的组合产生的平均抗张强度和弹性模量值分别提高了110%和133%。尽管两组之间的肿胀和细胞增殖测试结果没有显着差异,但考虑到体内分析的结果,双层支架的性能更好。还发现双层支架中的两层之间有良好的连接,从而使必要的操作和临床处理变得方便。
  • 【组织工程应用中电纺丝设计的最新进展:综述。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.36124 复制DOI
    作者列表:Kishan AP,Cosgriff-Hernandez EM
    BACKGROUND & AIMS: :Electrospinning, a technique used to fabricate fibrous scaffolds, has gained popularity in recent years as a method to produce tissue engineered grafts with architectural similarities to the extracellular matrix. Beyond its versatility in material selection, electrospinning also provides many tools to tune the fiber morphology and scaffold geometry. Recent efforts have focused on extending the capabilities of electrospinning to produce scaffolds that better recapitulate tissue properties and enhance regeneration. This review highlights these advancements by providing an overview of the processing variables and setups used to modulate scaffold architecture, discussing strategies to improve cellular infiltration and guide cell behavior, and providing a summary of electrospinning applications in tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2892-2905, 2017.
    背景与目标: :静电纺丝技术是一种用于制造纤维支架的技术,近年来作为生产与细胞外基质在结构上相似的组织工程移植物的一种方法而受到欢迎。除了在材料选择方面的多功能性之外,静电纺丝还提供许多工具来调整纤维的形态和支架的几何形状。最近的努力集中在扩展静电纺丝的能力上,以生产更好地概括组织特性并增强再生的支架。这篇综述通过概述用于调节支架结构的加工变量和设置,讨论了改善细胞浸润和指导细胞行为的策略,并提供了电纺技术在组织工程中的应用概述,突出了这些进展。分级为4 +©2017 Wiley Periodicals,Inc.J Biomed Mater Res Part A:105A:2892-2905,2017。
  • 【通过高速静电纺丝生产的单克隆抗体制剂。】 复制标题 收藏 收藏
    DOI:10.1016/j.ijpharm.2020.120042 复制DOI
    作者列表:Domján J,Vass P,Hirsch E,Szabó E,Pantea E,Andersen SK,Vigh T,Verreck G,Marosi G,Nagy ZK
    BACKGROUND & AIMS: :Solid formulations of monoclonal antibodies present several advantages, such as improved stability and increased shelf-life as well as simpler storage and transportation. In this study, we present a gentle drying technology for monoclonal antibodies, applying the water soluble 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) as matrix, to prepare a solid reconstitution dosage form. High-speed electrospinning of an aqueous infliximab-containing HP-β-CD solution was carried out at 25 °C resulting in fibers with an average diameter of 2.5 μm. The mAb-loaded electrospun fibers were successful to preserve the stability of infliximab in solid form. The results of size exclusion chromatography and gel electrophoresis indicated no significant increase in aggregate formation during the electrospinning process compared to the initial matrix solution. The binding activity of infliximab was preserved during electrospinning compared to the reference liquid formulation. Due to the enhanced surface area, excellent reconstitution capability, i.e. clear solution within 2 min without any vigorous mixing, could be achieved in a small-scale reconstitution test. The results of this work demonstrate that high-speed electrospinning is a very promising technique to manufacture the solid formulation of monoclonal antibodies for applications such as fast reconstitutable powders.
    背景与目标: :单克隆抗体的固体制剂具有多个优点,例如,改善的稳定性和更长的保质期以及更简单的存储和运输。在这项研究中,我们提出了一种单克隆抗体的温和干燥技术,该技术以水溶性2-羟丙基-β-环糊精(HP-β-CD)为基质,以制备固体重构剂型。在25°C下对含有英夫利昔单抗的HP-β-CD水溶液进行高速静电纺丝,得到平均直径为2.5μm的纤维。载有mAb的电纺纤维成功地保持了英夫利昔单抗的固体形式的稳定性。尺寸排阻色谱和凝胶电泳的结果表明,与初始基质溶液相比,在静电纺丝过程中聚集体的形成没有显着增加。与参考液体制剂相比,英夫利昔单抗的结合活性在静电纺丝过程中得以保留。由于表面积增加,因此在小型重构测试中可以实现出色的重构能力,即在2分钟内无任何剧烈混合的情况下获得澄清溶液。这项工作的结果表明,高速静电纺丝是一种非常有前途的技术,可用于制造单克隆抗体的固体制剂,以用于诸如快速可重构粉末的应用。
  • 【季铵化壳聚糖包衣的纳米纤维植入物通过电纺丝法制备,其负载棉酚及其对Graffi骨髓瘤的疗效。】 复制标题 收藏 收藏
    DOI:10.1080/09205063.2013.857543 复制DOI
    作者列表:Ignatova M,Kalinov K,Manolova N,Toshkova R,Rashkov I,Alexandrov M
    BACKGROUND & AIMS: :Nanofibrous poly(L-lactide-co-D,L-lactide) (coPLA) or coPLA/poly(ethylene glycol) implants loaded with plant polyphenolic compound gossypol (GOS) with anti-tumor activity were fabricated by electrospinning. Implants containing quaternized chitosan (QCh) were prepared by coating of the obtained fibrous materials with a thin film of cross-linked QCh. The morphology of the implants and chemical composition of the implant surface were studied by means of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). In vitro cytotoxicity assay showed that GOS-loaded nanofibrous implants, both non-coated and QCh-coated displayed about two-fold higher inhibitory activity against Graffi tumor cells than that of free GOS at the 72nd h of incubation. As evidenced by the performed fluorescence microscopy analyses and SEM observations, the anti-tumor activity of the fibrous implants was mainly due to induction of apoptosis. The experiments in which the implants containing both QCh and GOS were placed locally into the tumor site after the tumor extirpation showed an increase in the survival rate and a lower rate of recurrence in the operative field and of metastases in regional lymph nodes. In this case, 40% of hamsters were alive on the 45th day of implantation and they did not show any clinical sign of recurrence in the operative field and metastases in the regional lymph nodes.
    背景与目标: :通过电纺丝制备了负载有抗肿瘤活性的植物多酚化合物棉酚(GOS)的纳米纤维聚(L-丙交酯-co-D,L-丙交酯)(coPLA)或coPLA /聚乙二醇。含有季铵化壳聚糖(QCh)的植入物是通过在获得的纤维材料上涂覆一层交联的QCh薄膜来制备的。通过扫描电子显微镜(SEM)和X射线光电子能谱(XPS)研究了植入物的形态和植入物表面的化学成分。体外细胞毒性试验表明,在孵育的第72小时,未涂覆和QCh涂覆的GOS负载的纳米纤维植入物对Graffi肿瘤细胞的抑制活性比游离GOS约高两倍。如通过进行的荧光显微镜分析和SEM观察所证明的,纤维植入物的抗肿瘤活性主要归因于细胞凋亡的诱导。肿瘤切除后,将同时含有QCh和GOS的植入物局部放置到肿瘤部位的实验表明,其生存率提高了,手术区域的复发率降低了,局部淋巴结转移率也降低了。在这种情况下,有40%的仓鼠在植入后第45天还活着,并且在手术区域未见任何复发的临床迹象,在局部淋巴结也未见转移。
  • 【通过同轴电纺丝制备聚癸二酸甘油酯纤维膜:壳和核溶液的影响。】 复制标题 收藏 收藏
    DOI:10.1016/j.jmbbm.2016.06.022 复制DOI
    作者列表:You ZR,Hu MH,Tuan-Mu HY,Hu JJ
    BACKGROUND & AIMS: :Although poly(glycerol sebacate) (PGS) has enjoyed great success in soft tissue engineering, it remains challenging to fabricate PGS fibers. In this study, coaxial electrospinning, in which polylactide (PLA) was used to confine and draw PGS prepolymer, was used to fabricate PGS fibrous membranes. Specifically, effects of adding poly(ethylene oxide) (PEO), which was removed prior to curing, in the shell were investigated. Transmission and scanning electron microscopy were used to confirm core-shell structure and morphology of fibers, respectively. Both the removal of PEO or PLA in the shell and the efficacy of PGS curing were verified by Fourier transform infrared spectroscopy and differential scanning calorimetry. Mechanical properties of the membranes with different shell and core contents were examined. We found that the addition of PEO to the shell reduced Young׳s modulus of the resulting cured membrane and increased its elongation at break significantly, the latter indicating better PGS curing. Moreover, with the addition of PEO, increasing PGS prepolymer concentration further increased the elongation at break and appeared to enhance the structural integrity of fibers; PGS fibrous membranes (with no PLA shell) were thus successfully fabricated after the removal of PLA. The Young׳s modulus of the PGS fibrous membrane was ~0.47MPa, which is similar to that of PGS solid sheets and some soft tissues. Finally, the cytocompatibility of the electrospun membranes was validated by Alamar blue and LDH assays.
    背景与目标: :尽管聚癸二酸甘油酯(PGS)在软组织工程中获得了巨大的成功,但制造PGS纤维仍然具有挑战性。在这项研究中,使用聚乳酸(PLA)限制和拉伸PGS预聚物的同轴电纺丝用于制造PGS纤维膜。具体地,研究了在壳中添加在固化之前被除去的聚环氧乙烷(PEO)的效果。透射电子显微镜和扫描电子显微镜分别用于确认纤维的核-壳结构和形态。通过傅里叶变换红外光谱和差示扫描量热法验证了壳中PEO或PLA的去除以及PGS固化的有效性。检查了具有不同壳和芯含量的膜的机械性能。我们发现,在壳中添加PEO会降低所得固化膜的杨氏模量,并显着提高其断裂伸长率,后者表明PGS固化效果更好。而且,通过添加PEO,增加的PGS预聚物浓度进一步增加了断裂伸长率,并且似乎增强了纤维的结构完整性。因此,在除去PLA后成功地制备了PGS纤维膜(无PLA壳)。 PGS纤维膜的杨氏模量约为0.47MPa,与PGS固体薄片和一些软组织的杨氏模量相似。最后,通过Alamar蓝和LDH分析验证了电纺膜的细胞相容性。
  • 【通过新型电纺丝装置制造的3D双功能化神经导管的排列和随机纳米纤维的受控设计。】 复制标题 收藏 收藏
    DOI:10.1038/srep23761 复制DOI
    作者列表:Kim JI,Hwang TI,Aguilar LE,Park CH,Kim CS
    BACKGROUND & AIMS: :Scaffolds made of aligned nanofibers are favorable for nerve regeneration due to their superior nerve cell attachment and proliferation. However, it is challenging not only to produce a neat mat or a conduit form with aligned nanofibers but also to use these for surgical applications as a nerve guide conduit due to their insufficient mechanical strength. Furthermore, no studies have been reported on the fabrication of aligned nanofibers and randomly-oriented nanofibers on the same mat. In this study, we have successfully produced a mat with both aligned and randomly-oriented nanofibers by using a novel electrospinning set up. A new conduit with a highly-aligned electrospun mat is produced with this modified electrospinning method, and this proposed conduit with favorable features, such as selective permeability, hydrophilicity and nerve growth directional steering, were fabricated as nerve guide conduits (NGCs). The inner surface of the nerve conduit is covered with highly aligned electrospun nanofibers and is able to enhance the proliferation of neural cells. The central part of the tube is double-coated with randomly-oriented nanofibers over the aligned nanofibers, strengthening the weak mechanical strength of the aligned nanofibers.
    背景与目标: :由对齐的纳米纤维制成的脚手架由于其优越的神经细胞附着和增殖能力而有利于神经再生。然而,不仅要产生具有对齐的纳米纤维的整洁的垫子或导管形式,而且由于它们的机械强度不足而将它们用于外科手术作为神经引导导管也是具有挑战性的。此外,还没有关于在同一垫子上制造取向纳米纤维和随机取向的纳米纤维的研究的报道。在这项研究中,我们通过使用新型的静电纺丝技术成功地生产了具有取向和随机取向的纳米纤维的垫子。用这种改进的静电纺丝方法生产了一种具有高度对准的静电纺丝垫的新型导管,并将这种具有良好特性(如选择性渗透性,亲水性和神经生长定向操纵)的拟议导管制成了神经引导导管(NGC)。神经导管的内表面覆盖有高度排列的电纺纳米纤维,能够增强神经细胞的增殖。管的中央部分在取向的纳米纤维上被随机取向的纳米纤维双重涂覆,从而增强了取向的纳米纤维的弱机械强度。
  • 【一种具有通过同轴电纺丝形成的核-壳结构纳米纤维的血管组织工程支架及其生物相容性评价。】 复制标题 收藏 收藏
    DOI:10.1088/1748-6041/11/3/035007 复制DOI
    作者列表:Duan N,Geng X,Ye L,Zhang A,Feng Z,Guo L,Gu Y
    BACKGROUND & AIMS: :In this article, a tubular vascular tissue engineering scaffold with core-shell structured fibers was produced by coaxial electrospinning at an appropriate flow rate ratio between the inner and outer solution. PCL was selected as the core to provide the mechanical property and integrity to the scaffold while collagen was used as the shell to improve the attachment and proliferation of vascular cells due to its excellent biocompatibility. The fine core-shell structured fibers were demonstrated by scanning electron microscope and transmission electron microscope observations. Subsequently, the collagen shell was crosslinked by genipin and further bound with heparin. The crosslinking process was confirmed by the increasing of tensile strength, swelling ratio and thermogravimetric analysis measurements while the surface heparin content was characterized by means of a UV-spectrophotometer and activated partial thromboplastin time tests. Furthermore, the mechanical properties such as stitch strength and bursting pressure of the as-prepared scaffold were measured. Moreover, the biocompatibility of the scaffold was evaluated by cytotoxicity investigation with L929 cells via MTT assay. Endothelial cell adhesion assessments were conducted to reveal the possibility of the formation of an endothelial cell layer on the scaffold surface, while the ability of smooth muscle cell penetration into the scaffold wall was also assessed by confocal laser scanning microscopy. The as-prepared core-shell structured scaffold showed promising potential for use in vascular tissue engineering.
    背景与目标: :在本文中,通过在内部溶液和外部溶液之间的适当流速比下进行同轴电纺丝,生产了具有核-壳结构纤维的管状血管组织工程支架。选择PCL作为核心,以为支架提供机械性能和完整性,而由于其优异的生物相容性,将胶原蛋白用作外壳,以改善血管细胞的附着和增殖。通过扫描电子显微镜和透射电子显微镜观察证明了核壳结构细纤维。随后,胶原蛋白壳通过Genipin交联并进一步与肝素结合。交联过程通过提高抗张强度,溶胀率和热重分析法测定得到证实,而表面肝素含量则通过紫外分光光度计和活化的部分凝血活酶时间测试来表征。此外,测量了所制备的脚手架的机械性能,例如针脚强度和破裂压力。此外,通过MTT测定法对L929细胞进行细胞毒性研究,评价了支架的生物相容性。进行内皮细胞粘附评估以揭示在支架表面上形成内皮细胞层的可能性,同时还通过共聚焦激光扫描显微镜评估了平滑肌细胞渗透到支架壁中的能力。所制备的核-壳结构支架显示出在血管组织工程中使用的有希望的潜力。
  • 【静电纺丝法合成中空氧化铜及其在非酶化双氧水传感器中的应用。】 复制标题 收藏 收藏
    DOI:10.1016/j.colsurfb.2012.03.013 复制DOI
    作者列表:Wang B,Luo L,Ding Y,Zhao D,Zhang Q
    BACKGROUND & AIMS: :Cupric acetate/polyacrylonitrile composite nanofibers were prepared by electrospinning and hollow copper oxide (CuO) particles were produced after subsequent thermal treatment process. The electrospun hollow CuO particles modified carbon paste electrode (CPE) was demonstrated for the first time for nonenzymatic hydrogen peroxide (H(2)O(2)) sensor. The structures and morphologies of hollow CuO particles were characterized by scanning electron microscopy and X-ray diffraction spectrum. The assay performance of the modified sensor to H(2)O(2) was evaluated by cyclic voltammetry and amperometry, revealing high sensitivity (1746.50 μA mM(-1) cm(-2)), low detection limit (0.022 μM) and wide linear response of determination of H(2)O(2) oxidation in the range of 0.05 μM to 1.00 mM.
    背景与目标: :通过静电纺丝制备乙酸铜/聚丙烯腈复合纳米纤维,并在随后的热处理工艺后生产出中空的氧化铜(CuO)颗粒。电纺空心CuO颗粒改性碳糊电极(CPE)首次展示了非酶促过氧化氢(H(2)O(2))传感器。用扫描电子显微镜和X射线衍射光谱对中空CuO颗粒的结构和形貌进行了表征。改进的传感器对H(2)O(2)的测定性能通过循环伏安法和电流分析法进行评估,显示出高灵敏度(1746.50μAmM(-1)cm(-2)),低检测限(0.022μM)和H(2)O(2)氧化测定的宽线性响应,范围为0.05μM至1.00 mM。
  • 【通过电纺丝和热压花相结合制造的用于血管组织工程的聚合物支架。】 复制标题 收藏 收藏
    DOI:10.1088/1748-605X/aa8a81 复制DOI
    作者列表:Yan S,Zhang X,Zhang L,Liu H,Wang X,Li Q
    BACKGROUND & AIMS: :Although tissue engineering has shown great advances in recent years, creating proper mechanical properties and cell growth microenvironments is still challenging. In this study, electrospun nanofibrous membranes were hot embossed to develop three-dimensional hierarchical micro/nanostructures that load and culture human umbilical-vein endothelial cells (HUVECs). The hot-embossed membranes exhibited not only superior mechanical properties (the tensile strength was 7.01 ± 0.18 MPa and the tensile modulus was 166.91 ± 15.54 MPa), but also better cell viability evaluated through a CCK-8 assay and fluorescent dye. The grating and well arrays of the micropatterned fibre mats encouraged the HUVECs to proliferate. Therefore, the approach proposed in this paper-combined electrospinning and hot embossing-has bright prospects in biomedical applications for the use of polymer scaffold in tissue engineering.
    背景与目标: :尽管近年来组织工程学已显示出巨大的进步,但是创建适当的机械性能和细胞生长微环境仍然具有挑战性。在这项研究中,对静电纺丝纳米纤维膜进行热压印,以开发三维层次的微/纳米结构,从而加载和培养人脐静脉内皮细胞(HUVEC)。热压花膜不仅显示出优异的机械性能(拉伸强度为7.01±0.18 MPa,拉伸模量为166.91±15.54 MPa),而且还通过CCK-8分析和荧光染料评估了更好的细胞活力。带有微图案的纤维垫的光栅和孔阵列促进了HUVEC的扩散。因此,本文提出的将静电纺丝和热压花相结合的方法在生物医学应用中在组织工程中使用聚合物支架具有广阔的前景。
  • 【通过同轴静电纺丝将药物封装在可生物降解的超细纤维中。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.30564 复制DOI
    作者列表:Huang ZM,He CL,Yang A,Zhang Y,Han XJ,Yin J,Wu Q
    BACKGROUND & AIMS: :This article describes an electrospinning process to fabricate double-layered ultrafine fibers. A bioabsorbable polymer, Polycaprolactone (PCL), was used as the outer layer or the shell and two medically pure drugs, Resveratrol (RT, a kind of antioxidant) and Gentamycin Sulfate (GS, an antibiotic), were used as the inner layers or the cores. Morphology and microstructure of the ultrafine fibers were characterized by scanning electron microscope (SEM) and transmission electron microscopy (TEM), whereas mechanical performance of them was understood through tensile test. In vitro degradation rates of the nanofibrous membranes were determined by measuring their weight loss when immersed in pH 7.4 phosphate-buffered saline (PBS) mixed with certain amount of Pseudomonas lipase for a maximum of 7 days. The drug release behaviors of the RT and GS were measured using a high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-vis) spectroscopy, respectively. It has been found that the drug solutions without any fiber-forming additive could be encapsulated in the PCL ultrafine fibers, although they alone cannot be made into a fiber form. Beads on the fiber surface influenced the tensile behavior of the ultrafine fibers remarkably. When the core solvent was miscible with the shell solvent, higher drug concentration decreased the bead formation and thus favored the mechanical performance. The situation, however, became different if the two solvents were immiscible with each other. The degradation rate was closely related to hydrophilicity of the drugs in the cores. Higher hydrophilicity apparently led to faster degradation. The release profiles of the RT and GS exhibited a sustained release characteristic, with no burst release phenomenon.
    背景与目标: :本文介绍了静电纺丝工艺以制造双层超细纤维。外层或外壳使用可生物吸收的聚合物聚己内酯(PCL),内层使用两种医用纯药物白藜芦醇(RT,一种抗氧化剂)和硫酸庆大霉素(GS)。核心。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)表征了超细纤维的形貌和微观结构,而通过拉伸试验可以了解它们的机械性能。通过将纳米纤维膜浸入与一定量的假单胞菌脂肪酶混合的pH 7.4磷酸盐缓冲盐水(PBS)中,最长不超过7天的重量损失来确定其体外降解速率。 RT和GS的药物释放行为分别使用高效液相色谱(HPLC)和紫外可见光谱(UV-vis)进行了测量。已经发现,没有任何成纤维添加剂的药物溶液可以封装在PCL超细纤维中,尽管不能单独将它们制成纤维形式。纤维表面的珠子显着影响超细纤维的拉伸性能。当核溶剂与壳溶剂混溶时,较高的药物浓度会减少珠粒的形成,从而有利于机械性能。但是,如果两种溶剂互不相溶,情况就会变得不同。降解速率与药芯中的亲水性密切相关。较高的亲水性显然导致更快的降解。 RT和GS的释放曲线表现出持续释放特性,没有爆发释放现象。
  • 【通过乳液静电纺丝将桔红素包裹在PVA / PAA交联的电纺纤维中:形态表征,缓释和抗氧化活性评估。】 复制标题 收藏 收藏
    DOI:10.1016/j.foodchem.2020.127763 复制DOI
    作者列表:Zhan F,Yan X,Li J,Sheng F,Li B
    BACKGROUND & AIMS: :In this work, water-resistant poly (vinyl alcohol) (PVA)/poly (acrylic acid) (PAA) electrospun fibers encapsulating tangeretin (Tan) were fabricated by emulsion-electrospinning. To optimize the electrospinning condition, the size and morphology of electrospun fibers were characterized by dynamic light scattering (DLS), optical light microscope, fluorescence microscopy, and scanning electron microscopy (SEM), respectively. The optimized initial concentration of PVA/PAA was 10% (w/w) with a mass ratio of 3:7. The time and temperature of optimized thermal crosslinking treatment were 2 h and 145 °C, respectively. The results of XRD and SEM showed that the Tan was successfully incorporated into smooth PVA/PAA electrospun fibers and those fibers possessed nano-diameter size and high porosity. The encapsulation of Tan had no significant impact on the antioxidant activity of PVA/PAA/Tan crosslinking electrospun fibers. The in vitro release test showed the PVA/PAA/Tan crosslinking electrospun fibers achieved durable release profiles and lower burst release rates than that from the pure Tan emulsion. Based on these results, it is concluded that PVA/PAA/Tan crosslinking electrospun fibers prepared by emulsion-electrospinning serve as a promising technique in the fields of water-insoluble drug delivery and slow-release.
    背景与目标: :在这项工作中,通过乳液静电纺丝制备了包裹橘皮素(Tan)的耐水聚乙烯醇(PVA)/聚丙烯酸(PAA)电纺纤维。为了优化电纺条件,分别通过动态光散射(DLS),光学显微镜,荧光显微镜和扫描电子显微镜(SEM)对电纺纤维的尺寸和形态进行了表征。 PVA / PAA的最佳初始浓度为10%(w / w),质量比为3:7。优化的热交联处理的时间和温度分别为2 h和145°C。 XRD和SEM的结果表明,Tan成功地掺入了光滑的PVA / PAA电纺纤维中,并且这些纤维具有纳米直径的尺寸和高孔隙率。 Tan的包封对PVA / PAA / Tan交联电纺纤维的抗氧化活性没有明显影响。体外释放测试表明,与纯棕褐色乳液相比,PVA / PAA / Tan交联电纺纤维具有持久的释放特性和较低的破裂释放速率。基于这些结果,得出结论,通过乳液电纺丝制备的PVA / PAA / Tan交联电纺纤维在水不溶性药物递送和缓释领域中是有前途的技术。

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