BACKGROUND & AIMS: PURPOSE:The present study was aimed to evaluate the retinoprotective effects of Moringa oleifera (MO) in Streptozotocin-induced diabetic rats. METHODS:The study was continued for 24 weeks and evaluated for inflammatory (tumor necrosis factor [TNF]-α and interleukin [IL]-1β, angiogenic (vascular endothelial growth factor [VEGF] and protein kinase C [PKC]-β) and antioxidant (Glutathione, Superoxide dismutase, and Catalase) parameters. Retinal leakage was checked by Fluorescein angiography (FA) and fundus photographs were evaluated for retinal vessel caliber (arteriolar and venular). Transmission electron microscopy was done to determine basement membrane (BM) thickness. RESULTS:The results of the present study showed potential hypoglycemic and retinal antioxidant effects of MO. In the present study, a significant rise in the expression of retinal inflammatory (TNF-α and IL-1β) and angiogenic (VEGF and PKC-β) parameters was observed in diabetic retinae as compared to normal retinae. However, MO-treated retinae showed marked inhibition in the expression of inflammatory and angiogenic parameters. Further, in the present study, diabetic retinae showed dilated retinal vessels as compared to normal. However, MO-treated retinae showed marked prevention in the dilatation of retinal vessels. Fluorescein angiograms obtained from diabetic retinae showed leaky and diffused retinal vasculature. On the other hand, MO-treated retinae showed intact retinal vasculature. Further, results of the transmission electron microscopy study showed thickened capillary BM in the diabetic retina as compared to normal retinae. However, treatment with MO prevented thickening of capillary BM. CONCLUSION:Our result suggests that MO may be useful in preventing diabetes induced retinal dysfunction.

背景与目标： 目的：本研究旨在评估辣木（MO）对链脲佐菌素诱导的糖尿病大鼠的视网膜保护作用。
方法：研究持续了24周，并评估了炎症（肿瘤坏死因子[TNF]-α和白细胞介素[IL]-1β]，血管生成（血管内皮生长因子[VEGF]和蛋白激酶C [PKC]-β）和抗氧化剂（谷胱甘肽，超氧化物歧化酶和过氧化氢酶）参数，通过荧光素血管造影（FA）检查视网膜渗漏并评估眼底照片的视网膜血管口径（小动脉和小静脉），用透射电子显微镜确定基底膜（BM）的厚度。
结果：本研究结果显示了MO潜在的降血糖和视网膜抗氧化作用。在本研究中，与正常视网膜相比，在糖尿病视网膜中观察到了视网膜炎症表达（TNF-α和IL-1β）和血管生成（VEGF和PKC-β）参数的显着增加。然而，MO处理的视网膜在炎症和血管生成参数的表达中显示出明显的抑制作用。此外，在本研究中，与正常人相比，糖尿病视网膜显示出视网膜血管扩张。但是，MO处理的视网膜在视网膜血管扩张中显示出明显的预防作用。从糖尿病视网膜获得的荧光素血管造影照片显示渗漏和弥散性视网膜血管系统。另一方面，MO治疗的视网膜显示完整的视网膜脉管系统。此外，透射电子显微镜研究的结果表明与正常视网膜相比，糖尿病视网膜中的毛细血管BM增厚。但是，用MO处理可防止毛细血管BM增厚。
结论：我们的结果表明，MO可能有助于预防糖尿病引起的视网膜功能障碍。

BACKGROUND & AIMS: AIM:To identify the changes of mitochondrial protein expression in diabetic renal parenchyma and to characterize their molecular functions and biological processes in diabetes. METHODS:Mitochondrial proteins extracted from renal parenchyma mitochondria of streptozotocin-induced diabetic rats and normal rats were separated by two-dimensional polyacrylamide gel electrophoresis and identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry. RESULTS:Eleven proteins from 533 visualized protein spots displayed significant different expressions in mitochondria of diabetic kidneys compared with those in normal ones. Among these altered proteins, two proteins with the most obvious changes in protein expression were identified as alpha-2u globulin (mature protein, named A2) and its proteolytically modified form (named A2-fragment) respectively. These proteins were found in mitochondria of male rat renal parenchyma and were proved to be down-regulated in diabetic rats simultaneously. CONCLUSION:Our results suggest that down-regulation of alpha-2u globulin may be associated with an abnormal β-oxidation of long-chain fatty acids during diabetes. The decreased expression of A2-fragment in renal mitochondria of diabetic nephropathy may reduce fatty acid β-oxidation, which leads to a diminished energy supply from mitochondria to kidney tissue and the deposition of a large number of fatty acids in the kidney, ultimately causing and aggravating kidney damage. In conclusion, these findings may be helpful for understanding the molecular mechanism of diabetic nephropathy.

背景与目标： 目的：确定糖尿病肾实质中线粒体蛋白表达的变化，并对其在糖尿病中的分子功能和生物学过程进行表征。
方法：采用二维聚丙烯酰胺凝胶电泳分离从链脲佐菌素诱导的糖尿病大鼠和正常大鼠的肾实质线粒体中提取的线粒体蛋白，并通过基质辅助激光解吸/电离串联时间质谱法进行鉴定。
结果：533个可视化蛋白斑点中的11种蛋白在糖尿病肾线粒体中的表达与正常肾脏相比明显不同。在这些改变的蛋白质中，两个蛋白质表达变化最明显的蛋白质分别被鉴定为alpha-2u球蛋白（成熟蛋白质，称为A2）及其蛋白水解修饰形式（称为A2片段）。这些蛋白在雄性大鼠肾实质的线粒体中发现，并被证明在糖尿病大鼠中同时被下调。
结论：我们的结果表明，糖尿病患者中α-2u球蛋白的下调可能与长链脂肪酸的β-氧化异常有关。糖尿病肾病肾脏线粒体中A2片段的表达降低可能会降低脂肪酸β氧化，从而导致线粒体向肾脏组织的能量供应减少以及大量脂肪酸在肾脏中的沉积，最终导致和加重肾脏损害。总之，这些发现可能有助于理解糖尿病性肾病的分子机制。

BACKGROUND & AIMS: BACKGROUND:The aim of the present study was to evaluate the effects of low-level laser therapy (LLLT) on the healing of bone defects in rats with streptozotocin (STZ)-induced DM. METHODS:28 male Sprague-Dawley rats were used in this study. 14 animals received a single dose of STZ intraperitoneally (65 mg/kg) to induce Type I DM, whereas others were injected only with sterile saline solution. Four weeks later, standard bone defects were created in the tibiae of rats. Surgical wounds in one group from each of the diabetic and non-diabetic animals were irradiated with diode laser for every other day for 4 weeks and they were described as DM + LLLT and CONT + LLLT groups, respectively. Remaining two groups received no laser treatment. New bone formation, osteoblast and blood vessel counts were calculated in histologic sections. RESULTS:DM group had significantly smaller bone area and lower blood vessel count when compared to DM + LLLT, CONT and CONT + LLLT groups (p < 0.05 for each). CONT and CONT + LLLT groups had significantly larger bone area than DM + LLLT group (p < 0.05 for both). CONCLUSIONS:LLLT application promoted vascularization and new bone formation in animals with DM to a limited extent, since it was unable to support the healing process up to the level of non-diabetic animals.

背景与目标： 背景：本研究的目的是评估低剂量激光疗法（LLLT）对链脲佐菌素（STZ）诱导的DM大鼠骨缺损愈合的作用。
方法：采用28只雄性Sprague-Dawley大鼠。 14只动物腹膜内接受单剂STZ（65 mg / kg）诱导I型DM，而其他动物仅注射无菌盐溶液。四周后，在大鼠胫骨中形成了标准的骨缺损。每隔一天用二极管激光照射来自糖尿病和非糖尿病动物中每组的一组手术伤口，持续4周，分别称为DM LLLT组和CONT LLLT组。其余两组未接受激光治疗。在组织学切片中计算新的骨形成，成骨细胞和血管计数。
结果：与DM LLLT，CONT和CONT LLLT组相比，DM组的骨面积显着更小，血管计数更低（每组p <0.05）。 CONT和CONT LLLT组的骨面积明显大于DM LLLT组（两者均p <0.05）。
结论：LLLT的应用在一定程度上促进了DM动物的血管化和新骨形成，因为它不能支持非糖尿病动物的愈合过程。

BACKGROUND & AIMS: :Type 2 diabetes is a strong risk factor for stroke. Linagliptin is a dipeptidyl peptidase-4 (DPP-4) inhibitor in clinical use against type 2 diabetes. The aim of this study was to determine the potential antistroke efficacy of linagliptin in type 2 diabetic mice. To understand whether efficacy was mediated by glycemia regulation, a comparison with the sulfonylurea glimepiride was done. To determine whether linagliptin-mediated efficacy was dependent on a diabetic background, experiments in nondiabetic mice were performed. Type 2 diabetes was induced by feeding the mice a high-fat diet for 32 weeks. Mice were treated with linagliptin/glimepiride for 7 weeks. Stroke was induced at 4 weeks into the treatment by transient middle cerebral artery occlusion. Blood DPP-4 activity, glucagon-like peptide-1 (GLP-1) levels, glucose, body weight, and food intake were assessed throughout the experiments. Ischemic brain damage was measured by determining stroke volume and by stereologic quantifications of surviving neurons in the striatum/cortex. We show pronounced antistroke efficacy of linagliptin in type 2 diabetic and normal mice, whereas glimepiride proved efficacious against stroke in normal mice only. These results indicate a linagliptin-mediated neuroprotection that is glucose-independent and likely involves GLP-1. The findings may provide an impetus for the development of DPP-4 inhibitors for the prevention and treatment of stroke in diabetic patients.

背景与目标： ：2型糖尿病是中风的重要危险因素。 Linagliptin是临床上用于治疗2型糖尿病的二肽基肽酶4（DPP-4）抑制剂。这项研究的目的是确定利格列汀在2型糖尿病小鼠中的潜在抗中风功效。为了了解功效是否由血糖调节介导，与磺酰脲格列美脲进行了比较。为了确定利格列汀介导的功效是否取决于糖尿病背景，在非糖尿病小鼠中进行了实验。通过给小鼠喂高脂饮食32周来诱发2型糖尿病。用利格列汀/格列美脲治疗小鼠7周。在治疗的第4周，通过短暂性中脑动脉闭塞诱发中风。在整个实验过程中，评估了血液DPP-4活性，胰高血糖素样肽1（GLP-1）水平，葡萄糖，体重和食物摄入量。通过确定中风量和纹状体/皮层中存活的神经元的立体定量来测量缺血性脑损伤。我们在2型糖尿病和正常小鼠中显示了利格列汀的显着抗中风功效，而格列美脲仅在正常小鼠中被证明对中风有效。这些结果表明，由利格列汀介导的神经保护作用与葡萄糖无关，并且可能涉及GLP-1。这些发现可能为DPP-4抑制剂的开发提供动力，以预防和治疗糖尿病患者的中风。

BACKGROUND & AIMS: BACKGROUND:Second-generation antipsychotics are commonly associated with metabolic complications. These medications are being used more frequently for the treatment of mental health disorders in children, which has stimulated the need for creating formal guidelines on monitoring their safety and effectiveness. Previous guidelines have been developed for monitoring metabolic and neurological complications. To assist practitioners who perform these monitoring procedures, a complementary set of treatment recommendations have been created for situations in which abnormal measurements or results are encountered. OBJECTIVE:To create evidence-based recommendations to assist in managing metabolic complications in children being treated with second-generation antipsychotics. METHODS:A systematic review of the literature on metabolic complications of second-generation antipsychotic medications in children was conducted. Members of the consensus group evaluated the information gathered from the systematic review of the literature and used a nominal group process to reach a consensus on treatment recommendations. Wherever possible, references were made to existing guidelines on the evaluation and treatment of metabolic abnormalities in children. RESULTS:Evidence-based recommendations are presented to assist in managing metabolic complications including weight gain; increased waist circumference; elevation in prolactin, cholesterol, triglyceride and glucose levels; abnormal liver function tests and abnormal thyroid studies. CONCLUSION:The use of second-generation antipsychotics requires proper monitoring procedures. The present treatment guideline provides guidance to clinicians on the clinical management of metabolic complications if they occur.

背景与目标： 背景：第二代抗精神病药通常与代谢并发症相关。这些药物被更频繁地用于治疗儿童的精神疾病，这刺激了建立监测其安全性和有效性的正式指南的需求。已经开发了用于监测代谢和神经系统并发症的先前指南。为了帮助执行这些监视程序的从业人员，针对遇到异常测量或结果的情况创建了一套补充的治疗建议。
目的：建立基于证据的建议，以帮助管理接受第二代抗精神病药治疗的儿童的代谢并发症。
方法：对儿童第二代抗精神病药物的代谢并发症的文献进行了系统的综述。共识小组成员评估了从文献系统回顾中收集的信息，并使用名义上的小组程序就治疗建议达成共识。尽可能参考有关儿童代谢异常评估和治疗的现有指南。
结果：提出了循证医学建议，以帮助控制包括体重增加在内的代谢并发症。腰围增加；催乳激素，胆固醇，甘油三酸酯和葡萄糖水平升高；肝功能检查异常和甲状腺检查异常。
结论：使用第二代抗精神病药需要适当的监测程序。本治疗指南为临床医生就代谢并发症（如果发生）的临床管理提供了指导。

BACKGROUND & AIMS: INTRODUCTION:The role of the volume of radicular and dentigerous cysts on clinical symptoms is unclear and potentially may predict development of pre- and postoperative complications, especially after cystectomies with large bony defects. Therefore the aim of this study was to assess pre- and postoperative symptoms associated to the volume of jaw cysts. MATERIAL AND METHODS:Retrospective chart review of 111 patients with follicular or dentigerous cysts from 2008-2012. Anterior iliac crest grafts were used to fill defects after cystectomy. χ2-test was performed to analyze associations between two qualitative variables. Binary logistic regression analysis was used as multivariate analysis. P-values p < 0.05 were considered as significant. Volume measurement was performed in a semiautomatic segmentation method with the software "ITK-Snap". RESULTS:Postoperative hypaesthesia correlated significantly with histology (p = 0.025) and localization (p = 0.006). Volume was associated significantly with preoperative hypaesthesia (p = 0.052), postoperative hypaesthesia (p < 0.001), wound healing complications (p < 0.001) and length of wound healing complications (p = 0.001). Multivariate analysis identified volume as independent risk factor for postoperative hypaesthesia (p = 0.015). CONCLUSIONS:Volume analysis appears to be a method that allows risk stratification after surgery of jaw cysts. Therefore we recommend a precise treatment planning with the means of volume analysis to improve therapy outcome of patients with jaw cysts.

背景与目标： 引言：根管性和牙状囊肿的体积在临床症状上的作用尚不清楚，可能潜在地预测术前和术后并发症的发生，特别是在具有大骨缺损的膀胱切除术之后。因此，本研究的目的是评估与颌骨囊肿体积相关的术前和术后症状。
材料与方法：回顾性分析2008年至2012年间111例滤泡性或齿状囊肿患者的病历。膀胱切除术后，使用前terior骨移植物填补缺损。进行χ2检验以分析两个定性变量之间的关联。二元逻辑回归分析用作多元分析。 P值p <0.05被认为是显着的。使用软件“ ITK-Snap”以半自动分割方法进行体积测量。
结果：术后感觉低下与组织学（p = 0.025）和局限性（p = 0.006）显着相关。容量与术前感觉不足（p = 0.052），术后感觉不足（p <0.001），伤口愈合并发症（p <0.001）和伤口愈合并发症的时间（p = 0.001）显着相关。多变量分析确定了体积为术后感觉不足的独立危险因素（p = 0.015）。
结论：体积分析似乎是一种允许对颌骨囊肿进行手术后进行危险分层的方法。因此，我们建议采用体积分析的方法制定精确的治疗计划，以改善颌骨囊肿患者的治疗效果。

BACKGROUND & AIMS: :Thallium tomographic imaging and exercise electrocardiography were performed on 136 diabetic patients without symptoms of heart disease. Thirty three patients had post-exercise thallium defects and 19 had ST 1 mm greater than or equal to segment depression during exercise electrocardiography. Both tests were positive in 13 patients. Coronary angiography was subsequently performed on 33 patients with either scintigraphic and/or electrocardiographic evidence of myocardial ischaemia. Angiographically significant coronary artery disease (greater than or equal to 50% narrowing of the coronary artery lumen) was detected in 13 patients. Six patients had minimal coronary artery stenosis (less than 50%), and 14 had normal coronary arteries. Six patients refused cardiac catheterisation. In 14 out of 27 patients with post-exercise thallium defects coronary angiography did not show any coronary artery stenoses (positive predictive accuracy 48%). Exercise electrocardiography showed only one false positive result (positive predictive accuracy 94%) but failed to detect coronary artery disease in three patients with a positive scintigraphic result. The accuracy of a positive exercise electrocardiographic test seems to be better than that of a positive thallium tomographic scan for detecting asymptomatic coronary artery disease in diabetic patients. The high number of false positive thallium defects may be the result of technical features inherent in thallium tomography and/or the possible disease of the small intramyocardial arteries in diabetic patients.

背景与目标： ：对136例无心脏病症状的糖尿病患者进行了to体层断层显像和运动心电图检查。在运动心电图检查中，有33例运动后al缺陷患者，其中19例ST大于或等于节段压低1 mm。两项测试均阳性13例。随后对33例有心肌缺血的闪烁显像和/或心电图证据的患者进行了冠状动脉造影。在13例患者中发现了具有血管造影意义的冠状动脉疾病（大于或等于50％的冠状动脉腔狭窄）。 6例患者的冠状动脉狭窄程度最小（少于50％），而14例具有正常的冠状动脉。六名患者拒绝心脏导管插入术。在运动后th缺陷的27例患者中，有14例冠状动脉造影未显示任何冠状动脉狭窄（阳性预测准确性为48％）。运动心电图检查仅显示一个假阳性结果（阳性预测准确率94％），但在3个闪烁显像结果阳性的患者中未能检测到冠心病。积极的运动心电图检查的准确性似乎比阳性的X线体层摄影检查的准确性要好，以检测糖尿病患者的无症状冠状动脉疾病。大量假阳性positive缺陷可能是may断层扫描固有的技术特征和/或糖尿病患者心肌小动脉可能的疾病的结果。

BACKGROUND & AIMS: OBJECTIVE:We investigated the impact of two different injection strategies on the pharmacokinetics and pharmacodynamics of insulin aspart in vivo in an open-label, two-period crossover study and verified changes in the surface-to-volume ratio ex vivo. RESEARCH DESIGN AND METHODS:Before the clinical trial, insulin aspart was injected ex vivo into explanted human abdominal skin flaps. The surface-to-volume ratio of the subcutaneous insulin depot was assessed by microfocus computed tomography that compared 1 bolus of 18 IU with 9 dispersed boluses of 2 IU. These two injection strategies were then tested in vivo, in 12 C-peptide-negative type 1 diabetic patients in a euglycemic glucose clamp (glucose target 5.5 ± 1.1 mmol/L) for 8 h after the first insulin administration. RESULTS:The ex vivo experiment showed a 1.8-fold higher mean surface-to-volume ratio for the dispersed injection strategy. The maximum glucose infusion rates (GIR) were similar for the two strategies (10 ± 4 vs. 9 ± 4; P = 0.5); however, times to reach maximum GIR and 50% and 10% of the maximum GIR were significantly reduced by using the 9 × 2 IU strategy (68 ± 33 vs. 127 ± 93 min; P = 0.01; 38 ± 9 vs. 49 ± 16 min; P < 0.01; 23 ± 6 vs. 30 ± 10 min; P < 0.05). For 9 × 2 IU, the area under the GIR curve was greater during the first 60 min (219 ± 89 vs. 137 ± 75; P < 0.01) and halved until maximum GIR (242 ± 183 vs. 501 ± 396; P < 0.01); however, it was similar across the whole study period (1,361 ± 469 vs. 1,565 ± 527; P = 0.08). CONCLUSIONS:A dispersed insulin injection strategy enhanced the effect of a fast-acting insulin analog. The increased surface-to-volume ratio of the subcutaneous insulin depot can facilitate insulin absorption into the vascular system.

背景与目标： 目的：我们在开放标签，两期交叉研究中研究了两种不同的注射策略对体内门冬胰岛素的药代动力学和药效学的影响，并验证了离体表面积与体积比的变化。
研究设计和方法：在临床试验之前，将门冬胰岛素离体注射到植入的人腹部皮肤皮瓣中。皮下胰岛素贮存库的表面体积比是通过微焦点计算机断层扫描技术评估的，该技术比较了1个大剂量的18 IU与9个分散的大剂量的2 IU。然后在首次胰岛素给药后的正常血糖葡萄糖钳位（葡萄糖靶标5.5±1.1 mmol / L）中，在12名C肽阴性1型糖尿病患者体内测试了这两种注射策略。
结果：离体实验显示，分散注射策略的平均表面体积比高1.8倍。两种策略的最大葡萄糖输注速率（GIR）相似（10±4 vs. 9±4； P = 0.5）；但是，通过使用9×2 IU策略，达到最大GIR以及达到最大GIR的时间分别减少了50％和10％（68±33 vs. 127±93 min； P = 0.01； 38±9 vs. 49± 16分钟； P <0.01； 23±6 vs. 30±10分钟； P <0.05）。对于9×2 IU，在最初的60分钟内GIR曲线下的面积更大（219±89对137±75; P <0.01），并且减半直到最大GIR（242±183对501±396对; P < 0.01）;但是，在整个研究期间，情况相似（1,361±469对1,565±527； P = 0.08）。
结论：分散胰岛素注射策略增强了速效胰岛素类似物的作用。皮下胰岛素贮存库增加的表面积与体积之比可以促进胰岛素吸收进入血管系统。

BACKGROUND & AIMS: INTRODUCTION:Chronic lymphocytic leukemia (CLL) is a disorder of mature but dysfunctional monoclonal B cells. Microenvironment, antigenic stimulation and genetical mutations are demonstrated in etiopathogenesis. We aimed to evaluate the expression of CD11c in patients with CLL and its possible clinical significance. METHODS:Data of 259 patients with CLL between 2010 and 2016 in Trakya University Faculty of Medicine, including age at diagnosis, sex, whole blood count, stage, percentage of CLL cells in bone marrow, line of treatments, development of Richter's transformation and secondary tumors, autoimmune complications, IgG level, prognostic cytogenetic analysis, and length of survival were recorded from files. RESULTS:151 patients were male (58.3%) and 108 were male (41.7%). Mean age was 70 (21-92) years. CD11c was observed to be positive (>%20) in 103 patients (39.8%). Development of Richter's transformation, secondary tumors and ITP was significantly frequent in patients with CD11c positivity (P values .000, .003, .000 respectively). Also, IgG levels were significantly lower in this group (P = .000). Hemoglobin level, RAI stage and bone marrow CLL infiltration percentage were statistically related with CD11c (P values .036, .037, .000 respectively). Finally, CD11c was statistically related (in positive group 70 months, negative group 79 months, P = .001). CONCLUSION:CD11c, expressed not only in Hairy cell leukemia but also in dendritic cells, macrophages and monocytes is a differentiation marker for inflammation. Prolonged inflammation in the microenvironment of CLL cells may cause a susceptibility to autoimmune disorders and secondary tumors in CLL, in this way, an increase in mortality.

背景与目标： 简介：慢性淋巴细胞性白血病（CLL）是一种成熟但功能失调的单克隆B细胞疾病。微环境，抗原刺激和遗传突变已在病因学中得到证实。我们旨在评估CD11c在CLL患者中的表达及其可能的临床意义。
方法：特拉基亚大学医学院2010年至2016年间259例CLL患者的数据，包括诊断年龄，性别，全血细胞计数，分期，骨髓CLL细胞百分比，治疗方法，里氏转化的发展和继发性从文件中记录肿瘤，自身免疫并发症，IgG水平，预后细胞遗传学分析和生存期。
结果：男性151例，占58.3％；男性108例，占41.7％。平均年龄为70（21-92）岁。在103例患者中（39.8％）观察到CD11c阳性（>）。 CD11c阳性患者中Richter转化，继发性肿瘤和ITP的发生非常频繁（分别为P值.000，.003，.000）。此外，该组的IgG水平显着降低（P = .000）。血红蛋白水平，RAI分期和骨髓CLL浸润百分比与CD11c具有统计学相关性（P值分别为.036，.037和.000）。最后，CD11c具有统计学相关性（阳性组70个月，阴性组79个月，P = .001）。
结论：CD11c不仅在毛细胞白血病中表达，而且在树突状细胞，巨噬细胞和单核细胞中表达，是炎症的分化标志。 CLL细胞微环境中的长时间炎症可能会导致自身免疫性疾病和CLL中继发性肿瘤的易感性，从而增加死亡率。

BACKGROUND & AIMS: PURPOSE:To clarify whether endogenous peroxisome proliferator-activated receptor gamma (PPARgamma) and its ligand, rosiglitazone, affect retinal leukostasis and the associated vascular leakage using an experimental diabetic model. METHODS:Diabetes was induced in heterozygous PPARgamma+/- mice and Brown Norway rats with an intraperitoneal streptozotocin (STZ) injection. Retinal leukostasis and leakage, quantified by concanavalin A (Con A) lectin perfusion labeling combined with a fluorophotometric dextran leakage assay, were investigated at 120 days in diabetic PPARgamma+/- and wild-type mice and at 21 days in diabetic rats receiving rosiglitazone or the vehicle. The retinal protein expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-alpha, and the intercellular adhesion molecule (ICAM)-1 were investigated by means of the ELISA assay. RESULTS:In the diabetic PPARgamma+/- mice, retinal leukostasis and leakage were greater than in the diabetic wild-type mice. In addition retinal leukostasis and leakage were suppressed by treatment with rosiglitazone in experimental diabetic rats. ELISA analysis revealed that the upregulated ICAM-1 expression in the diabetic rat retina was reduced by rosiglitazone treatment. CONCLUSIONS:An endogenous pathway involving PPARgamma provides protection against retinal leukostasis and retinal leakage in diabetes and treatment with PPARgamma specific ligands inhibits retinal leukostasis and retinal leakage in diabetic rats.

背景与目标： 目的：使用实验性糖尿病模型来阐明内源性过氧化物酶体增殖物激活受体γ（PPARgamma）及其配体罗格列酮是否会影响视网膜白细胞形成和相关的血管渗漏。
方法：通过腹腔注射链脲佐菌素（STZ）在杂合性PPARγ/-小鼠和褐挪威大鼠中诱导糖尿病。通过伴刀豆球蛋白A（Con A）凝集素灌注标记结合荧光光度右旋糖酐泄漏测定定量研究视网膜白细胞的形成和渗漏，在糖尿病PPARγ/和野生型小鼠中在120天时以及在接受罗格列酮或糖尿病的糖尿病大鼠中在21天时进行了研究。车辆。 ELISA法检测了血管内皮生长因子（VEGF），肿瘤坏死因子（TNF）-α和细胞间黏附分子（ICAM）-1的视网膜蛋白表达水平。
结果：糖尿病PPARγ/-小鼠的视网膜白细胞增多和渗漏大于糖尿病野生型小鼠。此外，在实验性糖尿病大鼠中，通过罗格列酮治疗可抑制视网膜白细胞形成和渗漏。 ELISA分析显示，罗格列酮治疗可降低糖尿病大鼠视网膜中ICAM-1的表达。
结论：涉及PPARγ的内源性途径为糖尿病患者的视网膜白细胞增多和视网膜渗漏提供了保护，而PPARγ特定配体的治疗抑制了糖尿病大鼠的视网膜白细胞减少和视网膜渗漏。

BACKGROUND & AIMS: :Chronic hyperglycaemia in diabetes leads to the overproduction of free radicals and evidence is increasing that these contribute to the development of diabetic nephropathy. Among the spices, turmeric (Curcuma longa) is used as a flavouring and colouring agent in the indian diet every day and is known to possess anti-oxidant properties. The present study was designed to examine the effect of curcumin, a yellow pigment of turmeric, on renal function and oxidative stress in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by a single intraperitoneal injection of STZ (65 mg/kg) in rats. Four weeks after STZ injection, rats were divided into four groups, namely control rats, diabetic rats and diabetic rats treated with curcumin (15 and 30 mg/kg, p.o.) for 2 weeks. Renal function was assessed by creatinine, blood urea nitrogen, creatinine and urea clearance and urine albumin excretion. Oxidative stress was measured by renal malonaldehyde, reduced glutathione and the anti-oxidant enzymes superoxide dismutase and catalase. Streptozotocin-injected rats showed significant increases in blood glucose, polyuria and a decrease in bodyweight compared with age-matched control rats. After 6 weeks, diabetic rats also exhibited renal dysfunction, as evidenced by reduced creatinine and urea clearance and proteinuria, along with a marked increase in oxidative stress, as determined by lipid peroxidation and activities of key anti-oxidant enzymes. Chronic treatment with curcumin significantly attenuated both renal dysfunction and oxidative stress in diabetic rats. These results provide confirmatory evidence of oxidative stress in diabetic nephropathy and point towards the possible anti-oxidative mechanism being responsible for the nephroprotective action of curcumin.

背景与目标： ：糖尿病中的慢性高血糖症导致自由基的过度产生，并且越来越多的证据表明这些自由基有助于糖尿病性肾病的发展。在香料中，姜黄（姜黄）每天在印度饮食中用作调味剂和着色剂，并且已知具有抗氧化特性。本研究旨在检查姜黄素（一种姜黄黄色素）对链脲佐菌素（STZ）诱导的糖尿病大鼠肾功能和氧化应激的影响。在大鼠中通过腹膜内单次注射STZ（65 mg / kg）诱发糖尿病。注射STZ后四周，将大鼠分为四组，即对照大鼠，糖尿病大鼠和经姜黄素（15和30 mg / kg，口服）治疗2周的糖尿病大鼠。通过肌酐，血液尿素氮，肌酐和尿素清除率以及尿白蛋白排泄评估肾功能。通过肾脏丙二醛，还原型谷胱甘肽和抗氧化酶超氧化物歧化酶和过氧化氢酶来测定氧化应激。与年龄相称的对照大鼠相比，注射链脲佐菌素的大鼠显示血糖，多尿明显增加，体重下降。 6周后，糖尿病大鼠还表现出肾功能不全，这由肌酐和尿素清除率降低和蛋白尿以及氧化应激显着增加所证实，氧化应激由脂质过氧化作用和关键抗氧化酶的活性决定。姜黄素的慢性治疗可显着减轻糖尿病大鼠的肾功能不全和氧化应激。这些结果提供了糖尿病性肾病中氧化应激的确证证据，并指出可能的抗氧化机制负责姜黄素的肾保护作用。

BACKGROUND & AIMS: :This paper reports the proceedings of a consensus meeting on the incidence and complications of multiple gestation in Canada. In addition to background presentations about current and possible future practice in Canada, the expert panel also developed a set of consensus points. The need for infertility to be understood, and funded, as a healthcare problem was emphasized, along with recognition of the emotional impact of infertility. It was agreed that the goal of assisted reproduction treatment is the delivery of a single healthy infant and that even though many positive outcomes have resulted from twin or even triplet pregnancies, the potential risks associated with multiple pregnancy require that every effort be made to achieve this goal. The evidence shows that treatments other than IVF (such as superovulation and clomiphene citrate) contribute significantly to the incidence of multiple pregnancy. There is an urgent need for studies to understand better the usage and application of these other fertility technologies within Canada, as well as the non-financial barriers to treatment. The final consensus of the expert panel was that with adequate funding and good access to treatment, it will be possible to achieve the goal of reducing IVF-related multiple pregnancy rates in Canada by 50%.

背景与目标： ：本文报道了关于加拿大多胎妊娠的发生率和并发症的共识会议的会议记录。除了关于加拿大目前和将来可能的做法的背景介绍之外，专家小组还制定了一系列共识点。作为医疗保健问题，人们需要理解和资助不育症，并认识到不育症的情感影响。一致认为，辅助生殖治疗的目标是分娩一个健康的婴儿，即使双胞胎或什至三胞胎妊娠产生了许多积极的结果，但与多次妊娠相关的潜在风险仍需要尽一切努力来实现。目标。证据表明，除IVF以外的其他治疗方法（如超排卵和柠檬酸克罗米芬）对多胎妊娠的发生率有显着影响。迫切需要进行研究以更好地了解加拿大其他这些生育技术的使用和应用，以及治疗的非财务障碍。专家小组的最终共识是，只要有足够的资金和良好的治疗机会，就有可能实现将加拿大IVF相关的多胎妊娠率降低50％的目标。

BACKGROUND & AIMS: :Periacetabular osteotomy (PAO) is a well established method to treat hip dysplasia in the adult. There is, however, a lack of information on the subjective outcome of patients with complications after PAO. The purpose of this study was therefore to assess the influence of complications on the patients' post-operative wellbeing and function: 60 PAOs on 50 patients were investigated retrospectively after a mean follow-up of 7.4 years. The patients' self-reported assessment of health and function was evaluated by the Medical Outcomes Short Form-36 (SF-36) and the Western Ontario and McMaster Universities (WOMAC) questionnaires at last follow-up. Forty healthy persons served as a control group. Of the 60 interventions 13 had no complications. Minor complications occurred in 25 (41%) and in 22 (37%) at least one major complication occurred. SF-36 summary measure was 76.4 for PAO patients and 90.3 for the control group. Mean WOMAC score was 25.1. Patients with major complications had a similar subjective outcome as patients with minor or without complications, but persistent dysaesthesia due to lateral femoral cutaneous nerve dysfunction led to a worse subjective function. Lesions of the lateral femoral cutaneous nerve have much greater influence on patients' self-assessed functional outcome after PAO than previously reported and greater attention has to be given to this supposedly minor complication.

背景与目标： 小前截骨术（PAO）是一种成熟的治疗成人髋关节发育不良的方法。但是，缺乏有关PAO术后并发症患者的主观结果的信息。因此，本研究的目的是评估并发症对患者术后健康和功能的影响：在平均随访7.4年后，对50例患者的60个PAO进行了回顾性研究。在最后一次随访中，通过医学成果简表36（SF-36）以及西安大略和麦克马斯特大学（WOMAC）的问卷对患者的健康和功能自我评估进行了评估。 40名健康人作为对照组。在60项干预措施中，有13项没有并发症。轻微并发症发生在25（41％）和22（37％）中，至少发生了一次重大并发症。 SF-36总结指标为PAO患者为76.4，对照组为90.3。 WOMAC平均得分为25.1。具有严重并发症的患者的主观结局与具有轻微并发症或无并发症的患者相似，但由于股外侧皮神经功能障碍导致的持续感觉迟钝导致较差的主观功能。股外侧皮神经病变对PAO后患者自我评估的功能结局的影响要比以前报道的要大得多，因此必须对这种所谓的轻微并发症给予更大的关注。

BACKGROUND & AIMS: OBJECTIVE:Recent studies have demonstrated that 1,3-diacylglycerol (1,3-DAG) has several metabolic advantages over triacylglycerol (TAG) in humans and in animal models despite both oils having a similar fatty acid composition. In our current study, we have examined the effects of long-term feeding of a 1,3-DAG-rich oil on the dyslipidemia and atherosclerosis in the experimental model of the diabetic apolipoprotein E (apoE)-deficient mouse that develops accelerated atherosclerosis. METHODS AND RESULTS:Diets containing 1,3-DAG-rich oil or TAG oil were administered to control non-diabetic apoE-dificient and diabetic apoE-deficient mice for 20 weeks. In diabetic apoE-deficient mice, 1,3-DAG reduced the extent of atherosclerotic lesions in the aortic arch and thoracic aorta by 37 and 44%, respectively, compared to TAG. Further, in diabetic apoE-deficient mice, plasma total cholesterol and triglyceride levels were significantly lower in the 1,3-DAG-fed group than in the TAG-fed group. This occurred partially through an apparent reduction in the size of triglyceride-rich lipoproteins but not apparently by reducing the number of lipoprotein particles. By contrast the control non-diabetic apoE-deficient mice showed no differential responses to the type of oil at least over 20 weeks. CONCLUSIONS:We have demonstrated that dietary 1,3-DAG-rich oil reduced atherosclerosis in diabetic apoE-deficient mice, and was associated with reduction in plasma cholesterol especially within larger triglyceride-rich lipoproteins.

背景与目标： 目的：最近的研究表明，尽管两种油具有相似的脂肪酸组成，但在人和动物模型中，1，3-二酰基甘油（1，3-DAG）比三酰基甘油（TAG）具有若干代谢优势。在我们当前的研究中，我们在糖尿病性载脂蛋白E（apoE）缺陷型小鼠模型中，长期食用富含1,3-DAG的油对血脂异常和动脉粥样硬化的影响，这种小鼠会加速动脉粥样硬化。
方法和结果：给予含1,3-DAG丰富的油脂或TAG油脂的饮食，以控制非糖尿病apoE缺乏和糖尿病apoE缺乏的小鼠20周。在糖尿病apoE缺陷型小鼠中，与TAG相比，1,3-DAG分别将主动脉弓和胸主动脉的动脉粥样硬化病变程度降低了37％和44％。此外，在糖尿病apoE缺陷型小鼠中，1,3-DAG喂养组的血浆总胆固醇和甘油三酯水平显着低于TAG喂养组。这部分是由于富含甘油三酸酯的脂蛋白大小的明显减少而发生的，而不是由于减少脂蛋白颗粒的数量而明显发生的。相比之下，对照非糖尿病apoE缺陷型小鼠至少在20周内未显示出对油脂类型的差异反应。
结论：我们已经证明，富含饮食的1,3-DAG的油可以减少糖尿病apoE缺乏症小鼠的动脉粥样硬化，并且与血浆胆固醇的降低有关，尤其是在较大的富含甘油三酸酯的脂蛋白中。

BACKGROUND & AIMS: BACKGROUND:Airway anastomosis complications continue to be a source of morbidity for lung transplant recipients. METHODS:This study analyzes incidence, treatment, and follow-up of airway anastomotic complications occurring in 127 consecutive lung transplant airway anastomoses (77 single lung and 25 bilateral sequential lung). Complications were categorized as stenosis (11), granulation tissue (8), infection (7), bronchomalacia (5), or dehiscence (3). Follow-up after treatment ranged from 6 months to 4 years. RESULTS:Nineteen airway anastomosis complications (15.0%) occurred in 18 patients. Telescoping the airway anastomosis reduced the complication rate to 12 of 97 (12.4%), compared with 7 of 30 (23.3%) for omental wrapping, (p = 0.15). Complications developed in 13 of 77 single-lung airway anastomoses (16.9%) versus 6 of 50 bilateral sequential lung recipients (12.0%). Treatment consisted of stenting (9 airway anastomoses), bronchodilation (8), laser debridement (4), rigid bronchoscopic debridement (2), operative revision (2), and growth factor application (2). There was no difference in actuarial survival between patients with or without airway anastomosis complications (p = 1.0). CONCLUSIONS:Airway anastomosis complications can be successfully managed in the immediate or late postoperative period with good outcome up to 4 years after intervention.

背景与目标： 背景：气管吻合并发症仍然是肺移植接受者发病的来源。
方法：本研究分析了127例连续的肺移植气道吻合术（77例单肺和25例双侧序贯肺）发生的气道吻合并发症的发生率，治疗和随访情况。并发症分为狭窄（11），肉芽组织（8），感染（7），支气管软化（5）或裂开（3）。治疗后的随访时间为6个月至4年。
结果：18例患者发生了19例气道吻合并发症（15.0％）。伸缩气道吻合术使并发症发生率降低至97例中的12例（12.4％），而网膜包裹术的并发症发生率为30例中的7例（23.3％）（p = 0.15）。 77例单肺气道吻合中有13例发生了并发症（占16.9％），而50例双侧连续肺接受者中有6例发生了并发症（占12.0％）。治疗包括支架置入术（9例气道吻合术），支气管扩张术（8），激光清创术（4），硬支气管镜清创术（2），手术翻修（2）和生长因子应用（2）。有或没有气道吻合并发症的患者之间的精算生存率无差异（p = 1.0）。
结论：气道吻合并发症可在术后即刻或晚期成功治疗，干预后长达4年的效果良好。