Serum-free mouse embryo (SFME) cells, derived in medium in which serum is replaced with growth factors and other supplements, display distinctive properties: (i) SFME cells do not lose proliferative potential or show gross chromosomal aberration upon extended culture, (ii) these cells depend on epidermal growth factor for survival; and (iii) SFME cell proliferation is reversibly inhibited by serum. Treatment of SFME cells with serum or transforming growth factor beta led to the appearance of glial fibrillary acidic protein, a specific marker for astrocytes. The appearance of glial fibrillary acidic protein in cultures was reversed upon removal of transforming growth factor beta or serum. Cells with properties similar to SFME cells were also isolated from adult mouse brain. These results suggest a role for transforming growth factor beta in astrocyte differentiation in developing organisms and in response to injury and identify the cell type that has the unusual properties of SFME cells.

译文

无血清小鼠胚胎 (SFME) 细胞,在用生长因子和其他补充剂替换血清的培养基中衍生,显示出独特的特性 :( i) SFME细胞在延长培养后不会失去增殖潜力或显示出明显的染色体畸变,(ii) 这些细胞依赖表皮生长因子存活; (iii) 血清可逆地抑制SFME细胞增殖。用血清或转化生长因子 β 处理SFME细胞导致出现神经胶质纤维酸性蛋白,这是星形胶质细胞的特异性标志物。去除转化生长因子 β 或血清后,培养物中神经胶质纤维酸性蛋白的出现被逆转。还从成年小鼠大脑中分离出具有类似于SFME细胞特性的细胞。这些结果表明,转化生长因子 β 在发育中的生物体的星形胶质细胞分化和对损伤的反应中起作用,并鉴定具有SFME细胞异常特性的细胞类型。

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