• 【蛋白质二硫键异构酶相关多肽ERp60的人类基因的结构和经过加工的基因,并将这些基因分配到15q15和1q21。】 复制标题 收藏 收藏
    DOI:10.1006/geno.1997.4750 复制DOI
    作者列表:Koivunen P,Horelli-Kuitunen N,Helaakoski T,Karvonen P,Jaakkola M,Palotie A,Kivirikko KI
    BACKGROUND & AIMS: :ERp60 (also known as ERp61 or GRP58) is an isoform of protein disulfide isomerase (PDI) that has two thioredoxin-like domains a and a' in positions corresponding to those of domains a and a' in the PDI polypeptide and shows a significant amino acid sequence similarity to PDI in almost all parts. We report here that the human ERp60 gene is about 18 kb in size and consists of 13 exons. No distinct correlation was found between its exon-intron organization and the modular structure of the ERp60 polypeptide, nor were any similarities in exon-intron organization found between the human ERp60, PDI, and thioredoxin genes. The 5' flanking region of the ERp60 gene has no TATAA box or CCAAT motif but contains several potential binding sites for transcription factors. The highest levels of expression of the ERp60 mRNA were found by Northern blotting in the liver, placenta, lung, pancreas, and kidney, and the lowest in the heart, skeletal muscle, and brain. We also isolated an intronless ERp60 gene that probably represents a pseudogene. The ERp60 gene was mapped by fluorescence in situ hybridization to 15q15 and the processed gene to 1q21, so that neither was located on the same chromosome as the human PDI and thioredoxin genes.
    背景与目标: : ERp60 (也称为ERp61或GRP58) 是蛋白质二硫键异构酶 (PDI) 的同工型,具有两个硫氧还蛋白样结构域a和a',其位置与PDI多肽中的结构域a和a' 相对应,并且显示出显着的氨基酸序列在几乎所有部分中与PDI相似。我们在这里报告人类ERp60基因的大小约为18 kb,由13个外显子组成。在其外显子-内含子组织与ERp60多肽的模块化结构之间没有发现明显的相关性,在人ERp60,PDI和硫氧还蛋白基因之间也没有发现外显子-内含子组织有任何相似之处。ERp60基因的5' 侧翼区域没有TATAA盒或CCAAT基序,但包含几个潜在的转录因子结合位点。通过Northern印迹在肝脏,胎盘,肺,胰腺和肾脏中发现ERp60 mRNA的最高表达水平,而在心脏,骨骼肌和大脑中最低。我们还分离了一个可能代表假基因的无内含子ERp60基因。通过荧光原位杂交将ERp60基因定位到15q15,将处理后的基因定位到1q21,因此两者都与人PDI和硫氧还蛋白基因位于同一染色体上。
  • 【比较傅里叶变换红外研究细胞色素P-450cam和细胞色素P-420cam中的二级结构和CO血红素配体环境。】 复制标题 收藏 收藏
    DOI:10.1021/bi9700173 复制DOI
    作者列表:Mouro C,Jung C,Bondon A,Simonneaux G
    BACKGROUND & AIMS: For the first time, Fourier transform infrared spectroscopy has been applied to cytochrome P-450 to analyze the protein secondary structure. From Fourier self-deconvolution and fitting the infrared spectra in the amide I' region (1600-1700 cm-1), we estimate 44% alpha-helix, 31% beta-sheet, and 18% turns for substrate-free cytochrome P-450cam. In the presence of camphor, 54% alpha-helix and 310-helix, 21% beta-sheet, and 21% turns are obtained which agree with the crystallographic data of 53% alpha-helix, 19% beta-sheet, and 16% turns [Poulos, T. L., Finzel, B. C., & Howard, A. J. (1987) J. Mol. Biol. 195, 687-700]. Cytochrome P-420cam is produced from substrate-free cytochrome P-450cam in two ways(i) by temperature elevation up to 60 degrees C and (ii) by exposure to KSCN up to 1.5 M. The secondary structure composition is determined for each temperature and KSCN concentration and compared with the changes observed in the iron ligand CO stretch vibration bands appearing between 1900 and 2000 cm-1. Thermally induced cytochrome P-420 has an alpha-helix content of 19%, a beta-sheet content of 53%, 14% turns, and 5% antiparallel beta-sheets from intermolecular hydrogen bonds within protein aggregates. The formation of cytochrome P-420 as a function of the KSCN concentration indicates two types of cytochrome P-420. Up to 1 M KSCN, the induced cytochrome P-420 displays only little modification of the secondary structure, whereas at 1.5 M KSCN, larger changes are observed, resulting in 85% cytochrome P-420 without protein precipitation and containing 30% alpha-helix, 48% beta-sheet, and 17% turns. Infrared spectra in the iron ligand CO stretch region show several subconformers for cytochrome P-420. During the cytochrome P-420 formation, the CO stretch modes are shifted to higher frequencies by 3-11 cm-1, with a main feature at about 1964 cm-1, compared to those of substrate-free cytochrome P-450cam-CO.

    背景与目标: 首次将傅里叶变换红外光谱应用于细胞色素P-450,以分析蛋白质的二级结构。通过傅立叶自反卷积并拟合酰胺I' 区域 (1600-1700厘米-1) 中的红外光谱,我们估计了44% 无底物细胞色素P-450cam的 α-螺旋,31% β-折叠和18% 匝。在樟脑存在的情况下,获得了54% α-螺旋和310-螺旋,21% β-折叠和21% 匝,它们与53% α-螺旋,19% β-折叠和16% 匝的晶体学数据一致 [Poulos,T. L.,Finzel,b.c.,& Howard,A. J. (1987) J. Mol. Biol. 195,687-700]。细胞色素P-420cam以两种方式从无底物的细胞色素P-450cam产生 (i) 通过温度升高高达60 ℃ 和 (ii) 通过暴露于KSCN高达1.5 M。确定每种温度和KSCN浓度的二级结构组成,并将其与在出现1900年和2000厘米-1的铁配体CO拉伸振动带中观察到的变化进行比较。热诱导的细胞色素P-420具有19% 的 α-螺旋含量,53% 的 β-折叠含量,14% 转弯,以及来自蛋白质聚集体内的分子间氢键的5% 反平行 β-折叠。作为KSCN浓度的函数的细胞色素P-420的形成表明两种类型的细胞色素P-420。直到1 M KSCN,诱导的细胞色素P-420仅显示出很少的二级结构修饰,而在1.5 M KSCN处,观察到较大的变化,导致85% 的细胞色素P-420没有蛋白沉淀,并且含有30% 的 α-螺旋,48% 的 β-折叠和17% 转。铁配体CO拉伸区的红外光谱显示了几种用于细胞色素P-420的亚构象体。在细胞色素P-420形成过程中,与无底物细胞色素P-450cam-CO相比,CO拉伸模式向更高频率转移3-11厘米-1,主要特征约为1964厘米-1。
  • 【番红花ssp花粉萌发和柱头渗透的可逆抑制。用NO2,CO和O3气体熏蒸后的vernus (鸢尾科)。】 复制标题 收藏 收藏
    DOI:10.1055/s-2007-965246 复制DOI
    作者列表:Chichiriccò G,Picozzi P
    BACKGROUND & AIMS: :We assessed the pollen hydration, the pollen germination, and the stigma papilla penetration of CROCUS VERNUS subsp. VERNUS (Iridaceae) after 2 h fumigations with O (3), NO (2), and CO gases within humidified (90 - 100 % RH) box experiments. When the pollen and the pistil were separately fumigated, the pollen retained the capacity to emit a tube which penetrated papilla, and the stigma papillae retained the receptivity; when the pistils were first pollinated and then fumigated, the capacity of pollen to hydrate was not affected, but the germination was significantly reduced. The vulnerability to gases became evident at 0.3 ppm O (3), 0.2 ppm NO (2), and 0.5 ppm CO. The inhibition curves as a function of the gas concentrations were of an exponential type, and they saturated at 2 ppm NO (2), 25 ppm CO, and 0.5 ppm O (3), with germination percentages of 17 %, 27 %, and 60 %, respectively. Both the pollen germination and the papilla penetration were fully restored by prolonging for 60 - 90 min the incubation at 90 - 100 % RH, after the cessation of fumigations. The vulnerability of the pollen-papilla system is discussed.
    背景与目标: : 我们评估了番红花亚种的花粉水化,花粉萌发和柱头乳头渗透。在加湿 (90-100% RH) 盒实验中用O (3),NO (2) 和CO气体熏蒸2小时后的VERNUS (鸢尾科)。当花粉和雌蕊分别熏蒸时,花粉保留了穿透乳头的试管的能力,柱头乳头保留了感受性; 当雌蕊先授粉后熏蒸时,花粉的水合能力没有受到影响,但萌发明显降低。在0.3 ppm O (3) 、0.2 ppm NO (2) 和0.5 ppm CO时,对气体的脆弱性变得明显。作为气体浓度的函数的抑制曲线是指数型的,它们在2 ppm NO (2),25 ppm CO和0.5 ppm O (3) 下饱和,发芽率分别为17%,27% 和60%。熏蒸停止后,通过在90-100% RH下孵育60-90分钟,花粉萌发和乳头渗透都完全恢复。讨论了花粉-乳头系统的脆弱性。
  • 【接种量与底物比、底物混合比和接种量对草和猪粪分批共消化的影响。】 复制标题 收藏 收藏
    DOI:10.1016/j.biortech.2013.07.051 复制DOI
    作者列表:Dechrugsa S,Kantachote D,Chaiprapat S
    BACKGROUND & AIMS: :Biochemical methane potential (BMP) assay was conducted at 35 °C to evaluate the effects of inoculum to substrate ratio (ISR) and substrate mix ratio between para-grass and pig manure co-digesting using different inocula. Rubber latex digester (RLD) inoculum showed higher methanogenic activity (41.4 mL CH4/gVS) than pig farm digester (PFD) inoculum (37.3 mL CH4/gVS). However, the maximum methane yields, occurred at the highest para-grass mix ratio (G), were 369.6, 437.6, 465.9 and 442.6 mL CH4/gTSadded for RLD inoculum, versus 332.4, 475.0, 519.5 and 521.9 mL/gTSadded for PFD inoculum at ISR 1, 2, 3, and 4, respectively. HPr, HBu and HVa appeared at higher G, corresponding to substrate's higher biodegradability. Response surface indicated that higher ISR and G had a significantly positive impact on methane yield. It suggested the use of higher ISR, i.e. 3 or 4, for BMP assay of these co-substrates. Dominant species of fermentative bacteria in each inoculum was tested by DGGE.
    背景与目标: : 在35 °C下进行生化甲烷势 (BMP) 测定,以评估接种物与底物的比例 (ISR) 和底物混合比例在使用不同接种物的对位草和猪粪共同消化之间的影响。橡胶胶乳消化器 (RLD) 接种物显示出比猪场消化器 (PFD) 接种物 (37.3 mL CH4/gVS) 更高的产甲烷活性 (41.4 mL CH4/gVS)。然而,在最高的对草混合比 (G) 下,RLD接种物的最大甲烷产量为369.6、437.6、465.9和442.6 mL CH4/gts,而在ISR 1、2、3下,PFD接种物的最大甲烷产量为332.4、475.0、519.5和521.9 mL/gts,和4。HPr,HBu和HVa出现在较高的G处,对应于底物的较高的生物降解性。响应面表明,较高的ISR和G对甲烷产量有显着的积极影响。建议使用更高的ISR (即3或4) 进行这些共底物的BMP测定。通过DGGE测试了每个接种物中发酵细菌的优势种。
  • 【用于MRI的自动脑干共同配准 (ABC)。】 复制标题 收藏 收藏
    DOI:10.1016/j.neuroimage.2006.05.050 复制DOI
    作者列表:Napadow V,Dhond R,Kennedy D,Hui KK,Makris N
    BACKGROUND & AIMS: :Group data analysis in brainstem neuroimaging is predicated on accurate co-registration of anatomy. As the brainstem is comprised of many functionally heterogeneous nuclei densely situated adjacent to one another, relatively small errors in co-registration can manifest in increased variance or decreased sensitivity (or significance) in detecting activations. We have devised a 2-stage automated, reference mask guided registration technique (Automated Brainstem Co-registration, or ABC) for improved brainstem co-registration. Our approach utilized a brainstem mask dataset to weight an automated co-registration cost function. Our method was validated through measurement of RMS error at 12 manually defined landmarks. These landmarks were also used as guides for a secondary manual co-registration option, intended for outlier individuals that may not adequately co-register with our automated method. Our methodology was tested on 10 healthy human subjects and compared to traditional co-registration techniques (Talairach transform and automated affine transform to the MNI-152 template). We found that ABC had a significantly lower mean RMS error (1.22 +/- 0.39 mm) than Talairach transform (2.88 +/- 1.22 mm, mu +/- sigma) and the global affine (3.26 +/- 0.81 mm) method. Improved accuracy was also found for our manual-landmark-guided option (1.51 +/- 0.43 mm). Visualizing individual brainstem borders demonstrated more consistent and uniform overlap for ABC compared to traditional global co-registration techniques. Improved robustness (lower susceptibility to outliers) was demonstrated with ABC through lower inter-subject RMS error variance compared with traditional co-registration methods. The use of easily available and validated tools (AFNI and FSL) for this method should ease adoption by other investigators interested in brainstem data group analysis.
    背景与目标: : 脑干神经影像学中的组数据分析基于解剖结构的准确共配准。由于脑干由彼此密集相邻的许多功能异质核组成,因此在共同配准中相对较小的误差可能表现为检测激活的方差增加或敏感性降低 (或显着性)。我们设计了一种2阶段自动参考面罩引导的注册技术 (自动脑干共同注册或ABC),以改善脑干共同注册。我们的方法利用脑干掩模数据集对自动共同注册成本函数进行加权。我们的方法通过测量12个手动定义的地标上的均方根误差进行了验证。这些地标也被用作辅助手动共同注册选项的指南,该选项适用于可能无法与我们的自动方法充分共同注册的离群值个人。我们的方法在10名健康人类受试者上进行了测试,并与传统的共配准技术 (Talairach变换和自动仿射变换到MNI-152模板) 进行了比较。我们发现ABC的平均均方根误差 (1.22 +/-0.39毫米) 明显低于Talairach变换 (2.88 +/-1.22毫米,mu +/- sigma) 和全局仿射 (3.26 +/-0.81毫米) 方法。我们的手动地标引导选项 (1.51 +/-0.43毫米) 也提高了准确性。与传统的全球共配准技术相比,可视化单个脑干边界显示出ABC更加一致和统一的重叠。与传统的共配准方法相比,ABC通过较低的受试者间均方根误差方差证明了更高的鲁棒性 (对异常值的敏感性较低)。对于这种方法,使用易于获得和验证的工具 (AFNI和FSL) 应该可以简化对脑干数据组分析感兴趣的其他研究人员的采用。
  • 【量化共表达EGFR和HER2对HER活化和贩运的影响。】 复制标题 收藏 收藏
    DOI:10.1016/j.bbrc.2008.04.043 复制DOI
    作者列表:Shankaran H,Zhang Y,Opresko L,Resat H
    BACKGROUND & AIMS: :The human epidermal growth factor receptor (HER) system is an intricately regulated system that plays critical roles in development and tumorigenesis. Here, we apply integrated experimentation and modeling to analyze HER receptor activation in a panel of cell lines expressing endogenous levels of EGFR/HER1 and different levels of HER2. A mathematical model that includes the fundamental processes involved in receptor activation and trafficking was used to fit the experimental data, and values of the independent parameters for active receptor dimer formation affinities, trafficking rates and relative phosphorylation levels were estimated. Obtained parameter values quantitatively support the existing ideas on the effect of HER2 on EGFR dynamics, and enable us to predict the abundances of various phosphorylated receptor dimers in the cell lines.
    背景与目标: : 人类表皮生长因子受体 (HER) 系统是一个复杂的调节系统,在发育和肿瘤发生中起关键作用。在这里,我们应用综合实验和建模来分析表达内源性EGFR/HER1水平和不同水平her2的细胞系中的HER受体激活。使用包括受体激活和运输过程的基本过程的数学模型来拟合实验数据,并估算了活性受体二聚体形成亲和力,运输速率和相对磷酸化水平的独立参数值。获得的参数值定量地支持了关于HER2对EGFR动力学的影响的现有想法,并使我们能够预测细胞系中各种磷酸化受体二聚体的丰度。
  • 【麻醉的活跃研究领域: 麻醉文献的文献共引分析。】 复制标题 收藏 收藏
    DOI:10.1213/ane.0b013e31816d18a1 复制DOI
    作者列表:Jankovic MP,Kaufmann M,Kindler CH
    BACKGROUND & AIMS: BACKGROUND:The expansion of science has resulted in an increased information flow and in an exponentially growing number of connections between knowledge in different research fields. In this study, we used methods of scientometric analysis to obtain a conceptual network that forms the structure of active scientific research fields in anesthesia. METHODS:We extracted from the Web of Science (Institute for Scientific Information) all original articles (n = 3275) including their references (n = 79,972) that appeared in 2003 in all 23 journals listed in the Institute for Scientific Information Journal Citation Reports under the subject heading "Anesthesiology." After identification of highly cited references (> or = 5), pairs of co-cited references were created and grouped into uniformly structured clusters of documents using a single linkage and variable level clustering method. In addition, for each such cluster of documents, we identified corresponding front papers published in 2003, each of which co-cited at least two documents of the cluster core. Active anesthetic research fields were then named by examining the titles of the documents in both the established clusters and in their corresponding front papers. These research fields were sorted according to the proportion of recent documents in their cluster core (immediacy index) and were further analyzed. RESULTS:Forty-six current anesthetic research fields were identified. The research field named "ProSeal laryngeal mask airway" showed the highest immediacy index (100%) whereas the research fields "Experimental models of neuropathic pain" and "Volatile anesthetic-induced cardioprotection" exhibited the highest level of co-citation strength (level 9). The research field with the largest cluster core, containing 12 homogeneous papers, was "Postoperative nausea and vomiting." The journal Anesthesia & Analgesia published most front papers while Anesthesiology published most of the fundamental documents used as references in the front papers. CONCLUSIONS:Using co-citation analysis, we identified distinct homogenous clusters of highly cited documents representing 46 active current anesthetic research fields and determined multiple nets of knowledge among them.
    背景与目标:
  • 【使用新型共沉淀技术将双膦酸盐吸附到羟基磷灰石上,以增强骨生长。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.30792 复制DOI
    作者列表:McLeod K,Anderson GI,Dutta NK,Smart RS,Voelcker NH,Sekel R,Kumar S
    BACKGROUND & AIMS: :Premature bone resorption and remodeling by osteoclasts can limit the longevity of implant fixation and recovery time. Orally administered bisphosphonates (BPs) have been used to inhibit osteoclast action at the implant/bone interface. Ideally, these should be delivered at the interface with the osteoblast-active hydroxyapatite (HA) for maximum effect. This investigation introduces a novel BP loading technique to achieve improved BP release from a simulated body fluid-grown HA (SBF-HA) with the aim of improving implant fixation. A solution co-precipitation technique incorporates the BP (pamidronate) into a thin SBF-HA coating. Surface analysis, using X-ray photoelectron spectroscopy (XPS), of the resultant coating was employed to confirm the presence of the adsorbed BP on the surface of SBF-HA. XPS analysis was also used to determine the optimal adsorption process. Osteoclast cell culture experiments confirmed the biological effectiveness of BP adsorption and proved that the pamidronate was biologically active, causing both decreased osteoclast numbers and decreased resorption.
    背景与目标: : 破骨细胞过早吸收和重塑会限制植入物固定的寿命和恢复时间。口服双膦酸盐 (BPs) 已用于抑制植入物/骨界面处的破骨细胞作用。理想情况下,这些应在与成骨细胞活性羟基磷灰石 (HA) 的界面处递送,以获得最大效果。这项研究引入了一种新颖的BP加载技术,以改善模拟体液生长的HA (sbf-ha) 中的BP释放,目的是改善植入物的固定。溶液共沉淀技术将BP (帕米膦酸盐) 结合到薄的sbf-ha涂层中。使用x射线光电子能谱 (XPS) 对所得涂层进行表面分析,以确认sbf-ha表面上存在吸附的BP。XPS分析也用于确定最佳吸附过程。破骨细胞培养实验证实了BP吸附的生物学有效性,并证明帕米膦酸盐具有生物活性,导致破骨细胞数量减少和吸收减少。
  • 【合并症抑郁症会影响糖尿病相关费用吗?来自低收入国家横断面调查的证据。】 复制标题 收藏 收藏
    DOI: 复制DOI
    作者列表:Chereches RM,Litan CM,Zlati AM,Bloom JR
    BACKGROUND & AIMS: AIMS OF THE STUDY:The economic implications of co-morbid depression in patients with chronic medical disorders have been studied mainly in high-income countries. However, the applicability of such findings in developing countries cannot be assumed. In the present study we estimate diabetes related costs and explore the link between depression and diabetes related costs in Romania. In this former communist country, the general perception of practitioners and policy-makers is that psychological issues are far less important than medical concerns for patients with diabetes, a perception that may lead to the misallocation of already scarce resources. METHODS:Data related to costs of diabetes care and to co-morbid depression were collected from a sample of 1,171 diabetes patients at the Nutrition and Diabetes Center in Cluj-Napoca, Romania, using the Diabetes Costs Questionnaire (DCQ) and the Patient Health Questionnaire 9 (PHQ9). The gathered data were subjected to a bivariate analysis of the depression-cost relationship, as well as a regression analysis in order to isolate the effect of depression on diabetes related costs from the effect of covariates. RESULTS:Direct and indirect diabetes related costs equally contributed to the total costs. The repartition of the cost burden between the public system and private agents is nearly equal as well. The bivariate analysis of the depression-cost relationship reveals statistically significant larger diabetes related costs for patients with major depression than for patients with minor depression, and the latter have larger diabetes related costs than patients free of depression symptoms. When the pure effect of depression on diabetes related costs was isolated by means of regression techniques, the provisional diagnosis of major depression was found to significantly increase diabetes related costs. DISCUSSION:The equal distribution of diabetes related costs between direct and indirect measures, as well as the cost burden equally split between the public system and private agents can be explained by the costs of medication and the costs associated with time lost by the non-compensated caregivers. Consistent with Romanian cultural traditions, most of the patients rely on their relatives in an informal diabetes caregiving market for assistance. Alongside depression, the multivariate analysis revealed that factors such as Hungarian ethnicity, income, and number of years since diagnosis also significantly contribute to diabetes related costs. IMPLICATIONS FOR HEALTH CARE PROVISION AND USE:Findings that depression increases diabetes related costs bear potential implications for health policies and health care provision (i.e., the effect of depression on costs can be minimized by adequate recognition and treatment). As such, screening and treatment of co-occurring depression in diabetes patients should become part of the diabetes treatment protocol, not only in Romania but in other Central and Eastern European countries as well.
    背景与目标:
  • 【在颗粒状生物质反应器中对过量的啤酒酵母进行厌氧共消化,以提高生物甲烷的产量。】 复制标题 收藏 收藏
    DOI:10.1016/j.biortech.2012.08.064 复制DOI
    作者列表:Zupančič GD,Skrjanec I,Logar RM
    BACKGROUND & AIMS: :The anaerobic co-digestion of brewery yeast using granular biomass was studied on the lab, pilot and full-scale. The study shows no adverse effects in the co-digestion of yeast and wastewater in concentrations up to 1.1 (v/v)%. In concentrations up to 2.3% the process is manageable; however, not advisable. In concentrations over 2.8% the process exhibits failure due to the overload with suspended solids. An average specific biogas production of 0.560 m(3)kg(-1) of volatile solids was achieved. Full-scale operation with 0.7% yeast concentration showed a 38.5% increase in the biogas production and a 26.2% increase in the organic loading rate, which resulted in an increase of the biomethane/natural-gas substitute ratio from 10% to 16%. The influence of the yeast addition on the structure of the microbial biomass showed up to 7% dissimilarity in the archaeal and a 32% dissimilarity in the bacterial biomass community, which did not present any difficulties.
    背景与目标: : 在实验室,中试和全规模上研究了使用颗粒状生物质对啤酒酵母的厌氧共消化。该研究表明,在浓度高达1.1 (v/v)% 的酵母和废水的共消化中没有不利影响。在高达2.3% 的浓度下,该过程是可管理的; 然而,不可取。在超过2.8% 的浓度下,由于悬浮固体的过载,该过程表现出失败。实现了0.560 m(3)kg(-1) 挥发性固体的平均比沼气产量。0.7% 酵母浓度的全面操作显示出沼气产量的38.5% 增加和有机负载率的26.2% 增加,这导致生物甲烷/天然气替代率从10% 增加到16%。添加酵母对微生物生物量结构的影响在古细菌中显示出7% 的差异,而在细菌生物量群落中显示出32% 的差异,这没有任何困难。
  • 【大鼠枕叶皮质中共同表达p物质受体和GABA的神经元: 体内和器官型培养物之间的比较。】 复制标题 收藏 收藏
    DOI:10.1111/j.1460-9568.1997.tb01508.x 复制DOI
    作者列表:Echevarría D,Matute C,Albus K
    BACKGROUND & AIMS: The morphology and the distribution of neurons expressing the NK1-receptor (NK1R) and the co-expression of gamma-aminobutyric acid (GABA) in these neurons were studied in the rat occipital cortex and in organotypic cultures (OTCs) derived from this structure. By employing immunohistochemistry, we demonstrate that the NK1R-expressing neurons are non-pyramidal neurons and co-express GABA. Some differences were noted between in vivo and OTCs. NK1R-expressing neurons in OTCs had larger somata and longer dendrites and the proportion stained with an anti-GABA-antibody (approximately 50%) was smaller than in vivo (90%). The preferential location of NK1R-expressing neurons in layers II/III and VI, seen in vivo is not present in OTCs where these neurons distribute rather homogeneously through layers II-VI. Our findings imply that in contrast to the cat and monkey, in the rat occipital cortex the effects of substance P are almost exclusively mediated via inhibitory interneurons.

    背景与目标: 在大鼠枕叶皮层和衍生自该结构的器官型培养物 (otc) 中研究了表达NK1-receptor (NK1R) 的神经元的形态和分布以及 γ-氨基丁酸 (GABA) 的共表达。通过采用免疫组织化学,我们证明了NK1R-expressing神经元是非锥体神经元并且共表达GABA。注意到体内和otc之间的一些差异。Otc中的NK1R-expressing神经元具有较大的躯体和较长的树突,并且用抗GABA抗体染色的比例 (约50%) 小于体内 (90%)。在体内观察到的第II/III层和VI层中NK1R-expressing神经元的优先位置不存在于otc中,其中这些神经元相当均匀地分布在第ii-vi层中。我们的发现表明,与猫和猴子相反,在大鼠枕叶皮层中,p物质的作用几乎完全是通过抑制性中间神经元介导的。
  • 【通过加权基因共表达网络分析鉴定与卵巢癌铂类化疗耐药和治疗反应相关的模块和hub基因。】 复制标题 收藏 收藏
    DOI:10.1097/MD.0000000000017803 复制DOI
    作者列表:Zhang L,Zhang X,Fan S,Zhang Z
    BACKGROUND & AIMS: :High-grade serous ovarian carcinoma (HGSOC) is the most prevalent and malignant ovarian tumor.To identify co-expression modules and hub genes correlated with platinum-based chemotherapy resistant and sensitive HGSOC, we performed weighted gene co-expression network analysis (WGCNA) on microarray data of HGSOC with 12 resistant samples and 16 sensitive samples of GSE51373 dataset.A total of 5122 genes were included in WGCNA, and 16 modules were identified. Module-trait analysis identified that the module salmon (cor = 0.50), magenta (cor = 0.49), and black (cor = 0.45) were discovered associated with chemotherapy resistant, and the significance for these platinum-resistant modules were validated in the GSE63885 dataset. Given that the black module was validated to be the most related one, hub genes of this module, alcohol dehydrogenase 1B, cadherin 11, and vestigial like family member 3were revealed to be expressional related with platinum resistance, and could serve as prognostic markers for ovarian cancer.Our analysis might provide insight for molecular mechanisms of platinum-based chemotherapy resistance and treatment response in ovarian cancer.
    背景与目标: 高级别浆液性卵巢癌 (HGSOC) 是最常见和最恶性的卵巢肿瘤。为了确定与铂类化疗耐药和敏感的HGSOC相关的共表达模块和hub基因,我们对HGSOC的微阵列数据进行了加权基因共表达网络分析 (WGCNA),其中GSE51373数据集的12个耐药样品和16个敏感样品。WGCNA共包含5122个基因,并鉴定了16个模块。模块-性状分析发现模块鲑鱼 (cor  =   0.50) 、品红色 (cor  =   0.49) 和黑色 (cor  =   0.45) 与化疗耐药相关,并在GSE63885数据集验证了这些耐铂模块的意义。鉴于黑色模块被证实是最相关的一个,该模块的中枢基因,乙醇脱氢酶1B,钙粘蛋白11和残留样家族成员3被发现与铂类抗性表达相关,并可作为卵巢癌的预后指标,我们的分析可能为卵巢癌铂类化疗耐药的分子机制和治疗反应提供深入的研究。
  • 【断奶大鼠与热处理饮食 (美拉德反应) 有关的免疫能力。】 复制标题 收藏 收藏
    DOI:10.1079/bjn19970092 复制DOI
    作者列表:Varela P,del Mar Arce M,Marcos A,Castrillón AM
    BACKGROUND & AIMS: :Diets containing unheated casein (CD; control) or a casein-glucose mixture (CGD) previously heated at 140 degrees for 2 h were fed to two groups of young rats for 21 d. Differences in body weight, feed consumption, thymus, and spleen growth, protein metabolism and in vivo immune response were then determined. For this last experiment, animals were inoculated with sheep erythrocytes (SRBC) on day 15 to provide an immunological challenge. No changes were seen in body weight, feed consumption or feed conversion ratios. Neither were significant differences found in spleen weight, protein content, DNA content, DNase (EC 3.1.4.6) activity or lymphocyte count, suggesting that spleen cell growth remained similar in all the animals studied. The CGD induced marked increases in thymus DNA content whilst the protein:DNA ratio became lower. Spleen RNA content was similar in all rats, but thymus RNA content was 29% lower in the CGD group, although this difference did not reach statistical significance. This fact might be a consequence of the low RNase (EC 2.7.7.16) activity and RNase:RNA ratios in the thymus glands of CGD-fed animals. Further, the number of splenic plasma cells secreting anti-SRBC antibodies (direct plaque-forming cells) was significantly decreased in the same group. It might be concluded that both diets are adequate for rat growth and that the differences observed in the thymus of CGD-fed rats may be directed towards preserving tissue function. Nevertheless, the CGD did cause immunological disturbances affecting the humoral immune response.
    背景与目标: : 将含有未加热的酪蛋白 (CD; 对照) 或先前在140度加热2小时的酪蛋白-葡萄糖混合物 (CGD) 的饮食喂给两组幼鼠21 d。体重,饲料消耗,胸腺和脾脏生长,然后确定蛋白质代谢和体内免疫反应。对于最后一个实验,在第15天用绵羊红细胞 (SRBC) 接种动物以提供免疫学挑战。体重,饲料消耗或饲料转化率没有变化。脾脏重量,蛋白质含量,DNA含量,DNase (EC 3.1.4.6) 活性或淋巴细胞计数均未发现显着差异,这表明在所有研究的动物中,脾细胞的生长保持相似。CGD诱导胸腺DNA含量显着增加,而蛋白质: DNA比率降低。所有大鼠的脾脏RNA含量相似,但CGD组的胸腺RNA含量29% 较低,尽管这种差异没有达到统计学意义。这一事实可能是CGD喂养动物的胸腺中RNase (EC 2.7.7.16) 活性低和RNase:RNA比率低的结果。此外,在同一组中,分泌抗SRBC抗体的脾浆细胞 (直接斑块形成细胞) 的数量显着减少。可以得出结论,两种饮食都足以满足大鼠的生长,并且在CGD喂养的大鼠胸腺中观察到的差异可能是为了保留组织功能。然而,CGD确实引起了影响体液免疫反应的免疫紊乱。
  • 【阿尔及利亚蝙蝠中一种新型小核糖核酸病毒的遗传特征: 蝙蝠相关小核糖核酸病毒的共同进化分析。】 复制标题 收藏 收藏
    DOI:10.1038/s41598-019-52209-2 复制DOI
    作者列表:Zeghbib S,Herczeg R,Kemenesi G,Zana B,Kurucz K,Urbán P,Madai M,Földes F,Papp H,Somogyi B,Jakab F
    BACKGROUND & AIMS: :Bats are reservoirs of numerous zoonotic viruses. The Picornaviridae family comprises important pathogens which may infect both humans and animals. In this study, a bat-related picornavirus was detected from Algerian Minioptreus schreibersii bats for the first time in the country. Molecular analyses revealed the new virus originates to the Mischivirus genus. In the operational use of the acquired sequence and all available data regarding bat picornaviruses, we performed a co-evolutionary analysis of mischiviruses and their hosts, to authentically reveal evolutionary patterns within this genus. Based on this analysis, we enlarged the dataset, and examined the co-evolutionary history of all bat-related picornaviruses including their hosts, to effectively compile all possible species jumping events during their evolution. Furthermore, we explored the phylogeny association with geographical location, host-genus and host-species in both data sets.
    背景与目标: 蝙蝠是众多人畜共患病毒的储存库。小核糖核酸病毒科包括可能感染人类和动物的重要病原体。在这项研究中,该国首次从阿尔及利亚小optreus schreibersii蝙蝠中检测到与蝙蝠相关的小核糖核酸病毒。分子分析显示,新病毒起源于Mischi病毒属。在对获得的序列和有关蝙蝠小核糖核酸病毒的所有可用数据的操作使用中,我们对mischivirus及其宿主进行了共同进化分析,以真实地揭示该属中的进化模式。基于此分析,我们扩大了数据集,并检查了所有与蝙蝠相关的小核糖核酸病毒 (包括其宿主) 的共同进化历史,以有效地汇编其进化过程中所有可能的物种跳跃事件。此外,我们在两个数据集中都探索了系统发育与地理位置,寄主属和寄主物种的关联。
  • 【卵胞浆内单精子注射后完整的葡萄胎和共存的活胎儿: 1例报告并文献复习。】 复制标题 收藏 收藏
    DOI:10.1080/15513815.2019.1710790 复制DOI
    作者列表:Alpay V,Kaymak D,Erenel H,Cepni I,Madazli R
    BACKGROUND & AIMS: :Introduction: Twin pregnancy with complete hydatidiform mole and co-existing fetus (CHMCF) is an uncommon obstetric entity and may occur after assisted reproductive technologies. These pregnancies are associated with severe complications for both mother and fetus and the management is challenging. Case Report: We report a twin pregnancy after intracytoplasmic sperm injection (ICSI) treatment with CHMCF which delivered at 26 gestation weeks due to severe preeclampsia. The 625g neonate survived without any complication. The woman had persistent trophoblastic disease with lung metastasis and was treated with single agent methotrexate. We also present a brief review of the literature about the outcomes of CHMCF after ICSI. Conclusion: CHMCF may occur after ICSI treatment. Pregnancies with CHMCF are associated with severe complications however under close follow-up successful outcomes could be achieved in such pregnancies.
    背景与目标: 简介: 具有完全性葡萄胎和共存胎儿 (CHMCF) 的双胎妊娠是一种罕见的产科实体,可能发生在辅助生殖技术之后。这些怀孕与母亲和胎儿的严重并发症有关,并且管理具有挑战性。病例报告: 我们报告了因严重先兆子痫而在26个妊娠周接受CHMCF的卵胞浆内单精子注射 (ICSI) 治疗后的双胎妊娠。625g新生儿存活,没有任何并发症。该妇女患有持续性滋养细胞疾病并伴有肺转移,并接受了甲氨蝶呤单药治疗。我们还简要回顾了ICSI后CHMCF结果的文献。结论: ICSI治疗后可能发生CHMCF。CHMCF妊娠与严重并发症相关,但是在密切随访下,此类妊娠可以取得成功的结果。

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