OBJECTIVE:Concomitant cerebral infarction (CI) is could be a potential concern in experimental subarachnoid hemorrhage (SAH) induced by endovascular perforation. We propose a noninvasive method for excluding CI in a murine SAH model by using Laser speckle flow imaging (LSFI).
METHODS:An SAH was induced with endovascular perforation (EVP) in male ddY mice. The cerebral blood flow (CBF) was quantitatively measured in the bilateral cerebral cortex was performed by using LSFI at five timepoints (preprocedure, immediately after, and 3 hours, 6 hours, and 24 hours after the procedure). The mice were then euthanized, and the SAH grade and volume of the CI were evaluated. The mice were divided into the SAH group and the SAH + CI group. Differences between the groups were assessed.
RESULTS:Forty-eight mice were used in this study. Six were the sham control group. Five SAH mice died within 24 hours after the procedure. A large CI on the ipsilateral side occurred in 15 (40.5%) mice (i.e., SAH + CI group). The remaining 22 (59.5%) mice were classified as the SAH group. The SAH grading score was not significantly different between the groups. The neurological score and CBF of the ipsilateral hemisphere were significantly higher in the SAH group than in the SAH + CI group (neurological score: 12.3 vs. 8, p < 0.01; CBF: 343.1 vs. 205.5; p < 0.01). The cut-off modified neurological score for excluding CI was 8 (area under the curve [AUC]: 0.77) and CBF at 24 hours after the procedure was 279.2 (AUC:0.856).
CONCLUSIONS:Using LSFI is less invasive and effectively excludes concomitant CI in experimental SAH. This methodological protocol may ad in improving the quality of the EVP-SAH model.
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【Importance of CBF measurement to exclude concomitant cerebral infarction in the murine endovascular perforation SAH model.].
【在鼠血管内穿孔SAH模型中，CBF测量对排除伴随的脑梗塞的重要性。】
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DOI：10.1016/j.jstrokecerebrovasdis.2020.105243文章类型：杂志文章
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目的：并发脑梗死（CI）可能是血管内穿孔引起的实验性蛛网膜下腔出血（SAH）的潜在问题。我们提出了一种非侵入性的方法，通过使用激光散斑流成像（LSFI）排除鼠类SAH模型中的CI。
方法：用雄性ddY小鼠血管内穿孔（EVP）诱导SAH。在五个时间点（手术前，手术后，手术后3小时，6小时和24小时）使用LSFI定量测量双侧大脑皮层中的脑血流量（CBF）。然后对小鼠实施安乐死，并评估SAH等级和CI的体积。将小鼠分为SAH组和SAH CI组。评估组之间的差异。
结果：本研究使用了48只小鼠。假对照组为六个。手术后24小时内，五只SAH小鼠死亡。在15只（40.5％）小鼠（即SAH CI组）中发生了同侧较大的CI。其余的22只（59.5％）小鼠被归类为SAH组。两组之间的SAH评分没有显着差异。 SAH组同侧半球的神经学评分和CBF明显高于SAH CI组（神经学评分：12.3 vs. 8，p <0.01； CBF：343.1 vs. 205.5； p <0.01）。排除CI的临界改良神经学评分为8（曲线下面积[AUC]：0.77），手术后24小时的CBF为279.2（AUC：0.856）。
结论：在实验性SAH中使用LSFI具有较小的侵入性，并有效排除了伴随的CI。该方法学协议可能有助于提高EVP-SAH模型的质量。
方法：用雄性ddY小鼠血管内穿孔（EVP）诱导SAH。在五个时间点（手术前，手术后，手术后3小时，6小时和24小时）使用LSFI定量测量双侧大脑皮层中的脑血流量（CBF）。然后对小鼠实施安乐死，并评估SAH等级和CI的体积。将小鼠分为SAH组和SAH CI组。评估组之间的差异。
结果：本研究使用了48只小鼠。假对照组为六个。手术后24小时内，五只SAH小鼠死亡。在15只（40.5％）小鼠（即SAH CI组）中发生了同侧较大的CI。其余的22只（59.5％）小鼠被归类为SAH组。两组之间的SAH评分没有显着差异。 SAH组同侧半球的神经学评分和CBF明显高于SAH CI组（神经学评分：12.3 vs. 8，p <0.01； CBF：343.1 vs. 205.5； p <0.01）。排除CI的临界改良神经学评分为8（曲线下面积[AUC]：0.77），手术后24小时的CBF为279.2（AUC：0.856）。
结论：在实验性SAH中使用LSFI具有较小的侵入性，并有效排除了伴随的CI。该方法学协议可能有助于提高EVP-SAH模型的质量。
