The osmotolerant yeast Zygosaccharomyces rouxii is sensitive to the toxic L-proline analogue, L-azetidine-2-carboxylate (AZC). The possibility of use of the Saccharomyces cerevisiae MPR1 gene (ScMPR1) encoding the AZC-detoxifying enzyme as a dominant selection marker in Z. rouxii was examined. The heterologous expression of ScMPR1 in two Z. rouxii strains resulted in AZC-resistant colonies, but that of ScMPR1 as a dominant marker gene in vectors was affected by a high frequency of spontaneously resistant colonies. The same was found for an AZC-sensitive S. cerevisiae strain in which the ScMPR1 was expressed. In both yeasts, ScMPR1 can be used only as an auxiliary marker gene.
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【Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to L-azetidine-2-carboxylate.].
【酿酒酵母MPR1基因的编码N-乙酰基转移酶表达在鲁氏酵母中赋予了对L-氮杂环丁烷-2-羧酸盐的抗性。】
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DOI：10.1007/BF02932123文章类型：杂志文章
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耐渗透酵母酵母Zygosaccharomyces rouxii对有毒的L-脯氨酸类似物L-氮杂环丁烷-2-羧酸盐（AZC）敏感。研究了将酿酒酵母酿酒酵母MPR1基因（ScMPR1）编码为AZC解毒酶作为鲁氏沼虾的主要选择标记的可能性。 ScMPR1在两个鲁氏弧菌菌株中的异源表达导致了AZC抗性菌落，但作为载体中显性标记基因的ScMPR1的表达受到了高频率的自发抗性菌落的影响。对于其中表达ScMPR1的AZC敏感啤酒酵母菌株也发现了相同的情况。在两种酵母中，ScMPR1只能用作辅助标记基因。
