We examined the formation of hybrids of double-stranded DNA (dsDNA) and single-walled carbon nanotubes (SWNTs), which has not been well investigated yet. In particular, the adsorption of dsDNA onto SWNT produced by chemical vapor deposition (CVD) was examined for the first time. When small amount of dsDNA was mixed with CVD SWNT, well dispersed hybrids with smooth surfaces were observed by atomic force microscopy (AFM). Through a comparison of dsDNA, single-stranded DNA (ssDNA), CVD SWNT, and high-pressure carbon monoxide process (HiPco) SWNT, we found that the surface morphology of the hybrids was independent of the DNA type. Even when sonicated salmon testes DNA, which has a random sequence and length, was employed, smooth surfaces were obtained on the dsDNA-CVD hybrids as well as on the ssDNA-CVD hybrids. The ratio of monodispersed SWNT and bundled SWNT in a dispersion solution was also not affected by the DNA type. In contrast, the quantity of the fabricated hybrids was affected by the types of DNA especially when HiPco SWNT was used. Our results indicated that characteristic features of the dsDNA-CVD hybrids and provide an enhanced understanding of the adsorption mechanism of dsDNA onto SWNTs.

译文

:我们研究了双链DNA(dsDNA)和单壁碳纳米管(SWNTs)杂化物的形成,但尚未对此进行深入研究。特别地,首次检查了dsDNA在通过化学气相沉积(CVD)产生的SWNT上的吸附。当少量dsDNA与CVD SWNT混合时,通过原子力显微镜(AFM)观察到表面光滑且分散良好的杂种。通过比较dsDNA,单链DNA(ssDNA),CVD SWNT和高压一氧化碳工艺(HiPco)SWNT,我们发现杂种的表面形态与DNA类型无关。即使使用具有随机序列和长度的超声鲑鱼睾丸DNA,在dsDNA-CVD杂种以及ssDNA-CVD杂种上也可获得光滑的表面。分散液中单分散的SWNT和成束的SWNT的比例也不受DNA类型的影响。相反,所制备的杂种的数量受DNA类型的影响,尤其是在使用HiPco SWNT的情况下。我们的结果表明dsDNA-CVD杂化物的特征,并提供了对dsDNA吸附到SWNTs上的增强的理解。

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