BACKGROUND:Calligonum polygonoides L. subsp. comosum (L'Hér.) Sosk. is a plant species belonging to family Polygonaceae. Susceptibility to threaten, presence of various chemical constituents, and many medicinal effects reported for this plant in addition to rareness of in vitro culture studies have fuelled the need for its micropropagation and phytochemical investigations of the produced cultures.
OBJECTIVES:To employ in vitro culture technique for ex situ conservation of C. polygonoides, using the fruit as an explant; establish callus and cell suspension cultures from in vitro germinated plantlets; investigate the production of phenolics through callus, redifferentiated shoot, and cell suspension cultures; attempt to enhance cell capacity to accumulate phenolics using salicylic acid and yeast extract and provide a brief demonstration of biosynthetic pathway leading to phenolic production.
MATERIALS AND METHODS:Modified Murashige and Skoog media supplemented with growth hormones such as kinetin, 1-naphthaleneacetic acid, 6-benzylaminopurine, and indole-3-acetic acid were used to establish callus, redifferentiated shoots, and cell suspension cultures. Elicitation of cell suspension culture was performed using salicylic acid and yeast extracts. A reversed phase-high performance liquid chromatography method for determination of phenolic content in the aforementioned cultures was developed.
RESULTS:The unorganized callus and cell suspension cultures contained fewer amounts of phenolic compounds than redifferentiated shoots. Elicitation produced massive quantitative reprogramming of phenolic content.
CONCLUSION:The present study offers an alternative and renewable source for this valuable natural plant, provide a chance to improve secondary metabolite yield and serve as a useful tool for studying the biosynthesis of these compounds and its regulation in plant cells.
SUMMARY:In vitro culture techniques provided a strategy for ex situ conservation of the endangered C. polygonoides.Unorganized callus and cell suspension cultures accumulated less phenolic content than re-differentiated shoots.Elicitation produced massive quantitative reprogramming of phenolic content.Phenolic biosynthesis was discussed briefly. Abbreviation used: H2O2: Hydrogen peroxide, Kin: Kinetin, NAA: Naphthaleneacetic acid, BAP: 6-benzylaminopurine, IAA: Indole-3-acetic acid, HPLC: High-performance liquid chromatography.
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【Elicitation of Phenolics from the Micropropagated Endangered Medicinal Plant Calligonum polygonoides L. (Polygonoaceae).].
【从微繁殖的濒危药用植物Calligonum polygonoides L.（Polygonoaceae）中提取酚类。】
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DOI：10.4103/0973-1296.191458文章类型：杂志文章
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背景：刺五加亚种L.subsp。 comosum（L'Hér。）索斯克。是属于Poly科的一种植物。除了罕见的体外培养研究外，该植物还受到威胁的威胁，各种化学成分的存在以及据报道对该植物具有许多医学作用，这促使人们对其生产的培养物进行微繁殖和植物化学研究。
目的：采用体外培养技术，以果实为外植体，对多角隐孢子虫进行非原位保存。从体外发芽的苗中建立愈伤组织和细胞悬浮培养物；调查通过愈伤组织，再分化的芽和细胞悬浮培养物生产酚醛物质的方法；尝试使用水杨酸和酵母提取物增强细胞积累酚类物质的能力，并简要证明了导致酚类物质生产的生物合成途径。
材料与方法：改良的Murashige和Skoog培养基中添加了生长激素，例如激动素，1-萘乙酸，6-苄基氨基嘌呤和吲哚-3-乙酸，用于建立愈伤组织，再分化芽和细胞悬浮培养物。使用水杨酸和酵母提取物进行细胞悬浮培养的诱导。开发了一种用于测定上述培养物中酚含量的反相高效液相色谱法。
结果：无组织的愈伤组织和细胞悬浮培养物中所含的酚类化合物含量少于再分化的芽。引发产生大量的酚含量定量重编程。
结论：本研究为这种有价值的天然植物提供了可替代的可再生资源，为提高次生代谢产物的产量提供了机会，并为研究这些化合物的生物合成及其在植物细胞中的调控提供了有用的工具。
摘要：体外培养技术提供了一种濒危濒危梭状芽胞杆菌的非原生境保存策略。无组织的愈伤组织和细胞悬浮培养物积累的酚含量比再分化的芽少。诱导产生了大量的酚含量定量重编程。 。使用的缩写：H2O2：过氧化氢，Kin：Kinetin，NAA：萘乙酸，BAP：6-苄基氨基嘌呤，IAA：吲哚-3-乙酸，HPLC：高效液相色谱。
目的：采用体外培养技术，以果实为外植体，对多角隐孢子虫进行非原位保存。从体外发芽的苗中建立愈伤组织和细胞悬浮培养物；调查通过愈伤组织，再分化的芽和细胞悬浮培养物生产酚醛物质的方法；尝试使用水杨酸和酵母提取物增强细胞积累酚类物质的能力，并简要证明了导致酚类物质生产的生物合成途径。
材料与方法：改良的Murashige和Skoog培养基中添加了生长激素，例如激动素，1-萘乙酸，6-苄基氨基嘌呤和吲哚-3-乙酸，用于建立愈伤组织，再分化芽和细胞悬浮培养物。使用水杨酸和酵母提取物进行细胞悬浮培养的诱导。开发了一种用于测定上述培养物中酚含量的反相高效液相色谱法。
结果：无组织的愈伤组织和细胞悬浮培养物中所含的酚类化合物含量少于再分化的芽。引发产生大量的酚含量定量重编程。
结论：本研究为这种有价值的天然植物提供了可替代的可再生资源，为提高次生代谢产物的产量提供了机会，并为研究这些化合物的生物合成及其在植物细胞中的调控提供了有用的工具。
摘要：体外培养技术提供了一种濒危濒危梭状芽胞杆菌的非原生境保存策略。无组织的愈伤组织和细胞悬浮培养物积累的酚含量比再分化的芽少。诱导产生了大量的酚含量定量重编程。 。使用的缩写：H2O2：过氧化氢，Kin：Kinetin，NAA：萘乙酸，BAP：6-苄基氨基嘌呤，IAA：吲哚-3-乙酸，HPLC：高效液相色谱。
