• 【中国精神分裂症患者肥胖与健康相关生活质量的关系。】 复制标题 收藏 收藏
    DOI:10.3109/13651501.2012.745574 复制DOI
    作者列表:Guo X,Zhang Z,Zhai J,Wu R,Liu F,Zhao J,Early-stage Schizophrenia Outcome Study (ESOS) investigators.
    BACKGROUND & AIMS: OBJECTIVE:Studies have reported that up to 60% of individuals with schizophrenia are overweight or obese. This study explored the relationship between obesity and health-related quality of life (HRQoL) in Chinese patients with schizophrenia. METHODS:A total of 1,108 patients with schizophrenia aged 18-50 years were recruited from 10 different sites in China. Demographic and medical information were collected; the Mandarin version of Short Form 36 Health Survey questionnaire (SF-36) was used to assess HRQoL; in addition,height and weight were measured to calculate body mass index (BMI). BMI was categorized into underweight, normal weight, overweight and obese using cutoffs for Asian populations recommended by the World Health Organization. RESULTS:Fifty-six percent of participants with schizophrenia were overweight or obese. A higher BMI was associated with significantly lower scores in physical functioning, role-physical, and physical component summary (p's ≤ 0.010). Obese patients with schizophrenia had significantly lower scores in 3 domains and physical component summary of the SF-36 compared with normal weight patients (p's ≤ 0.007). CONCLUSION:Obesity is associated with decreased HRQoL in Chinese patients with schizophrenia. Our findings suggest that the prevention and management of weight gain and obesity is important in improving HRQoL in patients who suffer from this devastating mental illness.
    背景与目标:
  • 【中国青少年癫痫健康相关生活质量测量的因素结构和结构效度。】 复制标题 收藏 收藏
    DOI:10.1016/j.yebeh.2006.08.016 复制DOI
    作者列表:Yam WK,Ma DK,Cherk SW,Working Group for Multi-Centre Study on Quality of Life Measures for Youth with Epilepsy.
    BACKGROUND & AIMS: PURPOSE:Factor structure and construct validity of the Chinese version of the Health-Related Quality of Life Measure for Children with Epilepsy are reported. METHODS:The Parent-Proxy Response Scale and Child Self-Report Scale of the measure were administered to 266 children and their parents (or primary caregivers). Exploratory factor analysis was used to determine the underlying factor structure of the instrument. Hypothesis testing was used to explore construct validity. RESULTS:The Chinese version of the Health-Related Quality of Life Measure for Children with Epilepsy had the same five-factor structure as the Canadian version. Greater use of epilepsy-related services was associated with poorer quality of life. Quality of life was also poorer in children who had more seizures, took more than one anticonvulsant, had impaired cognition, and studied in special schools. Children who had close friends and spent more time in extracurricular activities with friends scored higher on the Interpersonal/Social subscale. CONCLUSIONS:Our data provide evidence of the cross-cultural applicability of the translated instrument.
    背景与目标:
  • 【c反应蛋白与老年人代谢综合征: 广州生物库队列研究。】 复制标题 收藏 收藏
    DOI:10.1016/j.atherosclerosis.2006.08.061 复制DOI
    作者列表:Lao XQ,Thomas GN,Jiang CQ,Zhang WS,Yin P,Adab P,Lam TH,Cheng KK
    BACKGROUND & AIMS: OBJECTIVE:To examine the association between serum c-reactive protein (CRP) concentration and the metabolic syndrome (MS) using the International Diabetes Federation Metabolic Syndrome Guidelines (April, 2005) definition in an older Chinese population. METHODS:Three thousand and fourteen men and 7275 women aged 50-85 years were recruited and received a full medical check-up including measurement of blood pressure, obesity indices, fasting total, LDL-, HDL-cholesterol, triglycerides, glucose and c-reactive protein. Data describing socioeconomic and lifestyle factors was also collected. RESULTS:Vascular risk factors including waist circumference, weight gain and waist gain since the age of 18, body mass index, waist-hip-ratio, triglyceride, HDL-cholesterol, and systolic and diastolic blood pressures were positively associated with CRP in both men and women (p from <0.001 to 0.016). Compared to those without MS components, adjusted odds ratios for having elevated CRP increased in those with the MS or its components compared those without MS or components (all p<0.001), except for the low HDL-cholesterol in both genders and raised fasting glucose in men. Similarly, the risk of elevated CRP increased with escalating MS components even after adjustment of a range of potential confounders. CONCLUSIONS:There is a strong relationship between CRP and the constellation of MS components and associated vascular risk in this older Chinese population.
    背景与目标:
  • 【TNFSF4基因多态性rs3861950而非rs3850641与中国人群脑梗死风险相关。】 复制标题 收藏 收藏
    DOI:10.1007/s11239-012-0849-9 复制DOI
    作者列表:Feng J,Liu YH,Yang QD,Zhu ZH,Xia K,Tan XL,Xia J,Gu WP,Zhou L,Xiao B,Tang BS,Huang Q
    BACKGROUND & AIMS: :Tumor necrosis factor superfamily member 4 (TNFSF4) plays a key role in the process of atherosclerosis, a common risk factor for both myocardial and cerebral infarctions. Recent studies indicate that the single nucleotide polymorphism (SNP) rs3850641 in TNFSF4 is associated with higher risk of myocardial infarction, but little is known about the association between TNFSF4 variation and cerebral infarction (CI). A case-control study involving 385 CI patients and 385 age-matched, sex-matched non-CI controls was conducted in a Chinese population, only the most common subtype, atherosclerosis CI, was recruited. Two SNPs of TNFSF4, rs3850641 and rs3861950, were genotyped by the TaqMan SNP genotyping method, and verified partly by genomic DNA sequencing. The results revealed a significant allelic association between rs3861950 and CI (Odds ration = 1.733, 95 % confidence interval = 1.333-2.254, P = 0.000). Genotypic association analysis demonstrated that the CC genotype of rs3861950 confers susceptibility to CI (Odds ration = 2.896, 95 % confidence interval = 1.368-6.132), and it was associated with a significantly higher risk of ischemic stroke (Odds ration = 3.520, 95 % confidence interval = 1.546-8.015, P = 0.003) after adjusting for the other confirmed risk factors such as the history of hypertension, diabetes, CAD, smoking and alcohol drinking. While the odds ratio of the T allele to the C allele was 1.733 (95 % confidence interval: 1.333-2.254). However, there was no significant association between rs3850641 and CI (Odds ration = 1.288, 95 % confidence interval = 0.993-1.670, P = 0.056). TNFSF4 gene polymorphism rs3861950, but not rs3850641, is associated with the risk of atherosclerosis CI in a Chinese population.
    背景与目标: : 肿瘤坏死因子超家族成员4 (TNFSF4) 在动脉粥样硬化过程中起关键作用,动脉粥样硬化是心肌和脑梗塞的常见危险因素。最近的研究表明,TNFSF4中的单核苷酸多态性 (SNP) rs3850641与心肌梗死的高风险相关,但对TNFSF4变异与脑梗死 (CI) 之间的关系知之甚少。在中国人群中进行了385例CI患者和385年龄匹配,性别匹配的非CI对照的病例对照研究,仅招募了最常见的亚型动脉粥样硬化CI。TNFSF4的两个SNP,rs3850641和rs3861950,通过TaqMan SNP基因分型方法进行了基因分型,并通过基因组DNA测序进行了部分验证。结果显示rs3861950和CI之间存在显着的等位基因关联 (赔率 = 1.733,95% 置信区间 = 1.333-2.254,P = 0.000)。基因型关联分析表明,rs3861950的CC基因型赋予CI易感性 (赔率 = 2.896,95% 置信区间 = 1.368-6.132),并且与缺血性中风的风险显着增加相关 (赔率 = 3.520,95% 置信区间 = 1.546-8.015,P = 0.003) 在调整其他已确认的危险因素 (例如高血压,糖尿病,CAD,吸烟和饮酒史) 后。而T等位基因与C等位基因的比值比为1.733 (95% 置信区间: 1.333-2.254)。然而,rs3850641与CI之间没有显着关联 (赔率 = 1.288,95% 置信区间 = 0.993-1.670,P = 0.056)。TNFSF4基因多态性rs3861950 (而非rs3850641) 与中国人群动脉粥样硬化CI的风险相关。
  • 【与核DNA相比,中国仓鼠卵巢细胞线粒体DNA中顺铂-DNA加合物的优先形成和去除减少。】 复制标题 收藏 收藏
    DOI:10.1016/s0165-1218(97)00037-2 复制DOI
    作者列表:Olivero OA,Chang PK,Lopez-Larraza DM,Semino-Mora MC,Poirier MC
    BACKGROUND & AIMS: Levels of DNA adducts in Chinese hamster ovary (CHO) cells exposed to cis-diamminedichloroplatinum(II) (cisplatin) for 24 h, have been shown to be 4- to 6-fold higher in mitochondrial (mt) DNA as compared to nuclear (n) DNA (Olivero et al., Mutation Res., 346 (1995) 221). The aim of the present study was to understand if the preferential cisplatin binding in mtDNA is partially caused by lack of adduct removal in the mitochondria. Chinese hamster ovary cells were exposed for 6 h to 50 microM cisplatin, followed by incubation for 24 and 48 h in cisplatin-free medium. At the 30-h time point (6 h with cisplatin, 24 h without cisplatin), half of the cells from each plate were harvested and the remainder were cultured and harvested at 54 h (6 h with cisplatin, 48 h without cisplatin). The 30- and 54-h time points are called 'T30' and 'T54', respectively. Cisplatin-DNA adducts were measured in DNA from nuclear and mitochondrial fractions by dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA), a sensitive competitive microtiter-based immunoassay utilizing antiserum elicited against cisplatin-modified DNA. An initial higher level of cisplatin-DNA adducts was observed in mtDNA when compared to nDNA, at T30. In addition, a lack of removal of adducts in mtDNA was demonstrated in cells at T54. Dilution of DNA adducts by DNA replication was documented in pulse-chase experiments that employed [3H]thymidine incorporation. Adduct removal by repair-related mechanisms was considered to comprise the difference between total DNA adduct removal and adduct removal related to DNA replication. The final results demonstrated that both, higher initial binding and lack of removal of cisplatin-DNA adducts appear to contribute to the preferential cisplatin-mtDNA binding observed in CHO cells.

    背景与目标: 暴露于顺式二甲基氯铂 (II) (顺铂) 24小时的中国仓鼠卵巢 (CHO) 细胞中的DNA加合物水平已显示为线粒体 (mt) DNA的4至6倍,比核 (n) DNA (Olivero等人,突变研究,346 (1995) 221)。本研究的目的是了解mtDNA中顺铂优先结合是否部分是由于线粒体中缺乏加合物去除而引起的。将中国仓鼠卵巢细胞暴露于50 microM顺铂中6小时,然后在无顺铂的培养基中孵育24和48小时。在30小时的时间点 (顺铂为6小时,无顺铂为24小时),从每个平板中收获一半的细胞,其余的细胞在54小时培养并收获 (顺铂为6小时,无顺铂为48小时)。30小时和54小时的时间点分别称为 “T30” 和 “T54”。通过解离增强的镧系元素氟免疫测定法 (DELFIA) 在核和线粒体级分的DNA中测量顺铂-DNA加合物,这是一种灵敏的基于微量滴定的竞争性免疫测定法,利用针对顺铂修饰的DNA的抗血清。与t30的nDNA相比,在mtDNA中观察到最初较高水平的顺铂-DNA加合物。此外,在t54细胞中显示出mtDNA中缺乏加合物的去除。DNA复制对DNA加合物的稀释在脉冲追踪实验中被记录,该实验采用 [3H] 胸苷掺入。修复相关机制的加合物去除被认为包括与DNA复制相关的总DNA加合物去除和加合物去除之间的差异。最终结果表明两者,更高的初始结合和缺乏顺铂-DNA加合物的去除似乎有助于在CHO细胞中观察到的顺铂-mtDNA优先结合。
  • 【中国赌场员工赌博混乱。】 复制标题 收藏 收藏
    DOI:10.1007/s10899-007-9068-2 复制DOI
    作者列表:Wu AM,Wong EM
    BACKGROUND & AIMS: :A previous study suggests that casino employees are at higher risk for disordered gambling than non-casino employees. The present study examined the cognitive correlates of the gambling involvement of Chinese casino employees. These potential cognitive correlates included attitudes toward the gaming industry and gambling activities, perceived job meaningfulness, and job stress. One hundred and nineteen Chinese respondents (M = 57; F = 62) working as dealers in Macao casinos were recruited through convenience sampling to fill out a questionnaire. The results revealed that about 7% of the respondents scored 10 or more on the South Oaks Gambling Screen and engaged in disordered gambling. Path analysis showed that attitude toward the gaming industry had a positive impact on job meaningfulness, which largely explained variances of job stress among casino employees. Job stress had a significant, but weak, direct impact on disordered gambling. Though causality between variables cannot be confirmed, this study provided insights into the impacts of cognitive factors on gambling involvement among Chinese front-line employees in the gaming industry. Implications of the findings were also discussed.
    背景与目标: : 先前的一项研究表明,赌场员工比非赌场员工有更高的赌博风险。本研究考察了中国赌场员工参与赌博的认知相关性。这些潜在的认知相关性包括对博彩业和赌博活动的态度,感知到的工作意义和工作压力。通过便利抽样招募了在澳门赌场担任交易商的119名中国受访者 (M = 57; F = 62),以填写问卷。结果显示,约7% 的受访者在South Oaks赌博屏幕上得分为10分或以上,并且从事无序赌博。路径分析表明,对博彩业的态度对工作意义产生了积极影响,这在很大程度上解释了赌场员工工作压力的差异。工作压力对无序赌博有显著但微弱的直接影响。尽管变量之间的因果关系无法确定,但本研究提供了认知因素对中国游戏行业一线员工赌博参与的影响的见解。还讨论了调查结果的含义。
  • 【在中国女性中,触珠蛋白基因的常见缺失与血液胆固醇水平有关。】 复制标题 收藏 收藏
    DOI:10.1038/jhg.2017.66 复制DOI
    作者列表:Zheng NS,Bastarache LA,Bastarache JA,Lu Y,Ware LB,Shu XO,Denny JC,Long J
    BACKGROUND & AIMS: :Haptoglobin (HP) protein plays a critical role in binding and removing free hemoglobin from blood. A deletion in the HP gene affects the protein structure and function. A recent study developed a novel method to impute this variant and discovered significant association of this variant with low-density lipoprotein (LDL) and total cholesterol levels among European descendants. In the present study, we investigated this variant among 3608 Chinese women. Consistent with findings from Europeans, we found significant associations between the deletion with lower cholesterol levels; women homozygous for the deletion allele (HP1-HP1), had a lower level of total cholesterol (-4.24 mg dl-1, P=0.02) and LDL cholesterol (-3.43 mg dl-1, P=0.03) than those not carrying the deletion allele (HP2-HP2). Especially, women carrying the HP1S-HP1S, had an even lower level of total cholesterol (-5.59 mg dl-1, P=7.0 × 10-3) and LDL cholesterol (-4.68 mg dl-1, P=8.0 × 10-3) compared to those carrying HP2-HP2. These associations remained significant after an adjustment for an established cholesterol level-related variant, rs2000999. Our study extends the previous findings regarding the association of HP structure variant with blood cholesterol levels to East Asians and affirms the validity of the new methodology for assessing HP structure variation.
    背景与目标: : 结合珠蛋白 (HP) 蛋白在结合和去除血液中的游离血红蛋白中起关键作用。HP基因的缺失会影响蛋白质的结构和功能。最近的一项研究开发了一种新颖的方法来估算该变体,并发现该变体与欧洲后代的低密度脂蛋白 (LDL) 和总胆固醇水平显着相关。在本研究中,我们调查了3608名中国女性的这种变异。与欧洲人的发现一致,我们发现缺失与较低的胆固醇水平之间存在显着关联; 缺失等位基因纯合子 (HP1-HP1) 的女性总胆固醇水平较低 (-4.24  mg   dl-1,P = 0.02) 和LDL胆固醇 (-3.43  mg   dl-1,P = 0.03) 比那些不携带缺失等位基因 (HP2-HP2)。特别是,携带HP1S-HP1S的妇女与携带HP2-HP2的妇女相比,总胆固醇 (-5.59  mg   dl-1,P = 7.0 × 10-3) 和LDL胆固醇 (-4.68  mg   dl-1,P = 8.0 × 10-3) 甚至更低。在对已建立的胆固醇水平相关变异体rs2000999进行调整后,这些关联仍然很重要。我们的研究将先前有关HP结构变异与血液胆固醇水平的关联的发现扩展到东亚人,并肯定了评估HP结构变异的新方法的有效性。
  • 【老年和脑积水与结核性脑膜炎患者预后不良相关: 一项针对中国成年人的回顾性研究。】 复制标题 收藏 收藏
    DOI:10.1097/MD.0000000000007370 复制DOI
    作者列表:Huang HJ,Ren ZZ,Dai YN,Tong YX,Yang DH,Chen MJ,Huang YC,Wang MS,Zhang JJ,Song WY,Pan HY
    BACKGROUND & AIMS: :Tuberculous meningitis (TBM) is the most common form of central nervous system tuberculosis with a very poor prognosis. We aimed at assessing risk factors related to the prognosis of patients with TBM.Forty-five inpatients with TBM in our institution from January 2013 to December 2015 were enrolled retrospectively. The good or poor prognosis in the patients was defined, based on Glasgow Outcome Scale System at discharge. Patients with a GOS score less than 5 were defined as "poor prognosis." Univariate and multivariate logistic regression analyses were performed to assess the predictors for TBM outcome.Among 45 TBM patients, 35 (77.8%) and 10 (22.2%) were in good, poor prognoses, respectively. Old age, disturbance of consciousness, moderate to severe electroencephalogram abnormality, hydrocephalus, remarkable increase of protein (≥ 236 mg/dL) and white blood cell counts (≥ 243 /μL) in cerebral spinal fluid were associated with poor prognosis. Multivariate analysis indicated that old age (odds ratio (OR) = 18.395, P = .036) and hydrocephalus (OR = 32.995, P = .049) were independent factors for a poor outcome of TBM.In conclusion, old age and hydrocephalus are the predictors for poor prognosis of TBM. Patients with these risk factors should be treated promptly with a special care paid to improve their outcomes.
    背景与目标: 结核性脑膜炎 (TBM) 是中枢神经系统结核病的最常见形式,预后很差。我们旨在评估与TBM患者预后相关的危险因素。我们机构从2013年1月到2015年12月的45例TBM住院患者被回顾性纳入。根据出院时格拉斯哥结局量表系统,确定了患者的良好或不良预后。GOS评分小于5的患者被定义为 “不良预后”。进行单因素和多因素logistic回归分析以评估TBM结果的预测因素。在45例TBM患者中,分别有35例 (77.8% 例) 和10例 (22.2% 例) 预后良好。年龄大、意识障碍、中重度脑电图异常、脑积水、脑脊液中蛋白质 (≥ 236  mg/dL) 和白细胞计数 (≥ 243  /μ l) 均与预后不良相关。多因素分析显示,高龄 (优势比 (OR)  =   18.395,p   =  .036) 和脑积水 (OR   =   32.995,p   =  .049) 是TBM预后不良的独立因素。老年和脑积水是TBM预后不良的预测因素。具有这些危险因素的患者应及时接受特殊护理治疗,以改善其预后。
  • 【中国城市社区老年人居家的发生及影响因素: 一项横断面研究。】 复制标题 收藏 收藏
    DOI:10.1097/MD.0000000000007207 复制DOI
    作者列表:Jing LW,Wang FL,Zhang XL,Yao T,Xing FM
    BACKGROUND & AIMS: :Studies on the occurrence of homebound and the factors influencing it are available. However, the study of community homebound in China is still in its preliminary stage. No previous studies about this issue are available. This study aims to assess the occurrence of and factors influencing homebound elderly in Chinese communities and to provide a basis for effective intervention and prevention of homebound elderly people.One sample community from three provinces was randomly selected. Investigations were performed on the selected communities and 2180 elderly people were chosen as the research subjects. Unified survey scales were used. Home visit and face-to-face interviews were performed to ensure that no single qualified survey respondent was missed.The rate of morbidity in homebound elderly Chinese community was found to be 15.49% and it gradually increased with age, and also with a lower education or poorer Activities of Daily Living (ADL). Single factor analysis showed that general situation, living habits, physical condition, mental condition, society, social support, and other factors affected the occurrence of community homebound elderly. Women were more likely to be homebound than men (P < .05). Having a spouse or high income reduced the rate of morbidity in the homebound elderly (P < .05). Multifactor regression analysis revealed that poor ADL, depression, hearing impairment, being old, no exercise, and low social support are the main influencing factors.Appropriate measures should be taken based on the specific influencing factor to prevent the occurrence of homebound.
    背景与目标: : 关于居家生活的发生及其影响因素的研究是可行的。然而,中国社区住房的研究仍处于初级阶段。以前没有关于这个问题的研究。本研究旨在评估中国社区居家老年人的发生情况及影响因素,为居家老年人的有效干预和预防提供依据。对选定的社区进行了调查,并选择了2180名老年人作为研究对象。使用了统一的调查量表。进行家访和面对面访谈,以确保没有一个合格的调查对象被遗漏。发现在家的中国老年人社区的发病率15.49%,并且随着年龄的增长而逐渐增加,并且受教育程度较低或日常生活活动 (ADL) 较差。单因素分析显示,一般情况、生活习惯、身体状况、精神状况、社会支持等因素影响社区居家老人的发生。女性比男性更有可能回家 (p  < .05)。有配偶或高收入降低了居家老人的发病率 (p < .05)。多因素回归分析显示,ADL差、抑郁、听力障碍、年老、无运动、社会支持低是主要影响因素,应根据具体影响因素采取相应措施,预防居家生活的发生。
  • 【跨年龄和文化的图片命名特异性: 针对年轻人和老年人的图片规范的潜在类分析。】 复制标题 收藏 收藏
    DOI:10.1159/000094611 复制DOI
    作者列表:Yoon C,Feinberg F,Gutchess AH
    BACKGROUND & AIMS: BACKGROUND:Cognitive aging research, as well as cross-cultural research, often relies on pictorial stimuli to address how perceptions of common objects vary by population group. OBJECTIVE:We investigate naming specificity--the degree of detail elicited for object labels - across age (younger-older) and culture (American-Chinese). METHODS:Using latent class analysis techniques, we segregated picture-item responses into multiple specificity levels. The analysis was conducted for 260 pictures, across four groups of younger (aged 17-25 years) and older (aged 59-76 years) adults in the US and mainland China. RESULTS:Overall, three naming specificity classes were supported. Age differences were modest compared to those across culture. In particular, Chinese groups showed far greater variation, naming more items both with high and with low specificity than age-matched American counterparts. Our results differed from prior studies using familiarity and latency measures. Moreover, approximately 28% of commonly-used picture items differed across all four groups. CONCLUSION:These results highlight the need to select appropriate pictorial stimuli for studies involving populations diverse in age and cultural background.
    背景与目标:
  • 【血清CEA,CA19-9和CA125对中国人群结直肠肝转移的预测价值。】 复制标题 收藏 收藏
    DOI:10.5754/hge121125 复制DOI
    作者列表:Zhang D,Yu M,Xu T,Xiong B
    BACKGROUND & AIMS: BACKGROUND/AIMS:Liver metastases are associated with poor prognosis in patients with colorectal cancer (CRC). The objective of this study is to determine the possible indicators in identifying the predictive value of serum CEA, CA19-9 and CA-125 in diagnosis of liver metastases from CRC in the Chinese population. METHODOLOGY:We randomly selected 101 CRC patients with liver metastases and 81 patients without liver metastases. Several clinical pathological factors were analyzed for the correlation with liver metastases. The predictive value of CEA, CA19-9 and CA-125 for liver metastases from CRC was evaluated. RESULTS:There was no significant difference in gender, age, hepatitis B history, serum AFP level or lesion location. Patients with liver metastases had a tendency to have higher serum CEA, CA19-9 and CA-125 level. Multivariate analysis revealed that serum CEA level (p<0.001), CA19-9 level (p<0.001) and CA-125 level (p=0.001) were independent prognostic predictors for liver metastases. Combination of CEA, CA19-9 and CA-125 can enhance their sensitivity in diagnosis of synchronous and metachronous liver metastases. Serum CA19-9 level, combined test of CA19-9 and CA-125, combined test of triple markers have higher sensitivities in synchronous metastasis than those in metachronous metastasis. CONCLUSIONS:Combination test would enhance the sensitivities of serum CEA, CA19-9 and CA-125 levels, which are important in predicting liver metastases from CRC in the Chinese population, either synchronous or metachronous.
    背景与目标:
  • 【5-HT2A受体基因启动子polymorphism-1438G/A与中国汉族人群神经性厌食症和精神病理特征的关系: 一项初步研究。】 复制标题 收藏 收藏
    DOI:10.1111/appy.12284 复制DOI
    作者列表:Kang Q,Chen J,Yu S,Yuan A,Zhang Y,Zhang R,Jiang W,Zhang C,Zhang H,Zhang M,Xiao Z
    BACKGROUND & AIMS: OBJECTIVE:The aim of the study was to explore the possible role of the 5-HT2A -1438G/A polymorphism in the susceptibility to anorexia nervosa (AN) in the Chinese Han population. METHODS:The -1438G/A polymorphism of 249 female AN patients, 228 matched healthy controls, and 198 trios was genotyped using SNaP shot assay. Psychopathological traits of eating-disordered behaviors in AN subjects were examined using the Chinese version of the Eating Disorder Examination Questionnaire. RESULTS:Neither the case-control analysis nor the transmission disequilibrium test revealed significant associations between the -1438G/A polymorphism and AN (P > .05). However, AA homozygote patients with AN had lower weight and shape concern scores of the EDE-Q6.0 than those of GA heterozygotes (P < .05). DISCUSSION:Our findings suggested that female AN patients with 5-HT2A -1438AA genotype may be characterized by less severe eating-disordered psychopathological traits in the Chinese Han population.
    背景与目标:
  • 【LRP1和A2M基因多态性与中国北方汉族人群阿尔茨海默氏病的关联。】 复制标题 收藏 收藏
    DOI:10.1016/j.jocn.2012.01.052 复制DOI
    作者列表:Yuan Q,Wang F,Xue S,Jia J
    BACKGROUND & AIMS: :Low-density lipoprotein receptor-related protein1 (LRP1) and alpha-2-macroglobulin (A2M) are candidate genes for sporadic Alzheimer's disease (SAD). It is not clear whether the LRP1 exon 3 and A2M exon 24 polymorphisms are associated with SAD. In the present study, we used direct sequencing to genotype the LRP1 C766T (rs1799986) polymorphism in exon 3 and the A2M I1000V (rs669) polymorphism in exon 24 in 364 patients with SAD and 291 healthy control subjects from the Northern Chinese Han population. The distributions of LRP1 genotypes (chi-squared [χ(2)]=7.25, degrees of freedom [d.f.]=2, p=0.027) and alleles (χ(2)=8.154, d.f.=1, p=0.004) were significantly different between patients and controls who were apolipoprotein E (APOE) ε4 positive. The T allele and TT+TC genotype were associated with a reduced risk of developing SAD (T allele: odds ratio [OR]=0.541, 95% confidence interval [CI]=0.368-0.859, p=0.005; TT+TC genotype: OR=0.613, 95% CI=0.315-0.725, p=0.012). There was no statistically significant difference in allele and genotype frequencies between patients with SAD and control subjects for the A2M I1000V polymorphism, even after stratification by age of onset, gender, and APOE ε4 status. We found an interaction between LRP1 and APOE genotypes (p=0.001), but no interaction between LRP1 and A2M genotypes. Our results suggest that the T allele of the LRP1 C766T polymorphism is associated with a decreased risk of SAD in APOE ε4 carriers from the Northern Han Chinese population.
    背景与目标: : 低密度脂蛋白受体相关蛋白1 (LRP1) 和alpha-2-macroglobulin (A2M) 是散发性阿尔茨海默氏病 (SAD) 的候选基因。目前尚不清楚LRP1外显子3和A2M外显子24多态性是否与SAD相关。在本研究中,我们使用直接测序对来自中国北方汉族人群的364名SAD患者和291名健康对照受试者的外显子3中的LRP1 C766T (rs1799986) 多态性和外显子24中的A2M I1000V (rs669) 多态性进行了基因型。LRP1基因型 (卡方 [χ(2)]= 7.25,自由度 [d.f.]= 2,p = 0.027) 和等位基因 (χ(2)= 8.154,d.f.= 1,p = 0.004) 在载脂蛋白E (APOE) ε4阳性的患者和对照组之间有显着差异。T等位基因和TT + TC基因型与发展SAD的风险降低相关 (T等位基因: 优势比 [OR]= 0.541,95% 置信区间 [CI]= 0.368-0.859,p = 0.005; TT + TC基因型: OR = 0.613,95% CI = 0.315-0.725,p = 0.012)。即使按发病年龄,性别和apoeε4状态分层后,SAD患者和对照组的A2M I1000V多态性的等位基因和基因型频率也没有统计学上的显着差异。我们发现LRP1和APOE基因型之间存在相互作用 (p = 0.001),但LRP1和A2M基因型之间没有相互作用。我们的结果表明,LRP1 C766T多态性的T等位基因与中国北方汉族人群apoeε4携带者的SAD风险降低有关。
  • 【通过HLA-DRB1和HLA-DRB3 ∗ 02之间的基因间重组鉴定了一个新的DRB1等位基因。】 复制标题 收藏 收藏
    DOI:10.1016/j.humimm.2013.07.013 复制DOI
    作者列表:Huang W,Liu X,Li E,Zhao C,Liu Q,Liang Z,Wang Y,Lu F
    BACKGROUND & AIMS: :In this study, a novel DRB1 allele was revealed by routine HLA-SBT typing noted for its extensive mismatches to any known DRB1 alleles within the exon 2. Sequences containing the exons 2, 3 of HLA-DRB1, their surrounding introns, and the full-length cDNA of DRB1 were analyzed to determine a possible recombination event. Interestingly, the sequences of entire exon 2 were characterized as DRB3(∗)02:02:01:01/02; while exon 3 were characterized as DRB1(∗)14 like alleles. Further analysis of the sequences using Simplot software suggested that an intergenic recombinant event (i.e. exchange of sequence between non-allelic genes) may have occurred between DRB3(∗)02 allele and DRB1(∗)14 like allele, and the recombination sites are located at intron 1 and the boundary of exon 2 and intron 2 of DRB1. There are 5 CGGGG sequences flanking each side of exon 2 could serve as potential recombination site. Moreover, the full-length cDNA of the novel allele has been identified. The exon 1 and exon 3 to exon 6 share the same sequence as DRB1(∗)14 like alleles. At the mRNA level, the new allele has no significant difference when compared with the other DRB1 allele. This novel recombinant allele is also found to be paternally inherited. In conclusion, this is the first report of a DRB1 and DRB3 intergenic recombination event involving whole exon 2, which generate a new DRB1(∗)14:141.
    背景与目标: : 在这项研究中,通过常规hla-sbt分型揭示了一种新的DRB1等位基因,该分型因其与外显子2内任何已知的DRB1等位基因的广泛错配而闻名。分析包含HLA-DRB1的外显子2、3、它们周围的内含子和DRB1的全长cDNA的序列以确定可能的重组事件。有趣的是,整个外显子2的序列被表征为DRB3(∗)02:02:01:01/02; 而外显子3被表征为DRB1(∗)14类似等位基因。使用Simplot软件对序列的进一步分析表明,可能在DRB3(∗)02等位基因和DRB1(∗)14等位基因之间发生了基因间重组事件 (即非等位基因之间的序列交换),重组位点位于drb1的内含子1和外显子2和内含子2的边界。外显子2两侧有5个CGGGG序列可以作为潜在的重组位点。此外,已经鉴定出新等位基因的全长cDNA。外显子1和外显子3至外显子6与DRB1(∗)14的等位基因共享相同的序列。在mRNA水平上,新等位基因与其他DRB1等位基因相比没有显着差异。这种新的重组等位基因也被发现是父系遗传的。总之,这是涉及整个外显子2的DRB1和DRB3基因间重组事件的首次报道,该事件产生了新的DRB1(∗)14:141。
  • 【中国冠心病患者的活化蛋白C比率,血浆组织因子活性和活化因子VII。】 复制标题 收藏 收藏
    DOI: 复制DOI
    作者列表:He WJ,Hu Y,Zhang XP,Hong M,Guo T,Wei WN,Song SJ
    BACKGROUND & AIMS: OBJECTIVE:There is close relationship between abnormal coagulation system and progression of coronary heart disease (CHD), our purpose is to evaluate the contribution of hematologic factors and some other risk factors to the development of coronary heart disease (CHD) in Chinese population. METHODS:56 patients with CHD at admission and 54 controls were enrolled. Plasma levels of protein C, free protein S, total protein S, thrombomodulin, activated factor VII (FVIIa), factor VII:Ag, P-selectin, tissue-type plasminogen activator, plasminogen activator inhibitor-1 were measured by enzyme linked immunosorbent assay, activity of tissue factor (aTF) by chromogenic activity assay, and activated protein C (APC) ratio, prothrombin time, aPTT, fibrinogen, D-dimmer and thrombin time by full-automated coagulation analyzer. RESULTS:Compared with controls, plasma level of thrombomodulin, FVIIa, factor VII:Ag and aTF were raised in CHD group (p<0.05, 0.001, 0.05, and 0.05, respectively). The average APC ratio in CHD group was lower than that in controls (p<0.001). The result of binary logistic regression analysis showed that activated factor VII (OR2.680, 95%CI1.539-4.665) and tissue factor activity (OR1.019, 95%CI1.004-1.035) were risk factors and high density lipoprotein (OR0.008, 95%CI0-0.478) and activated protein C ratio (OR0.001, 95%CI0-0.011) were protective factors for CHD. CONCLUSIONS:Low activated protein C ratio, elevated tissue factor activity and increased activated factor VII in plasma may contribute to development of coronary heart disease.
    背景与目标:

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