Here, a novel mycovirus, Botryosphaeria dothidea mitovirus 1 (BdMV1), was isolated from a phytopathogenic fungus, Botryosphaeria dothidea, and its molecular characteristics were determined. BdMV1 has a genome of 2,667 nt that contains a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 727 amino acids with a molecular mass of 81.64 kDa. BLASTp analysis revealed that the RdRp domain of BdMV1 has 39.59% and 39.18% sequence identity to Plasmopara viticola associated mitovirus 43 and Setosphaeria turcica mitovirus 1, respectively. Phylogenetic analysis further suggested that BdMV1 is a new member of the genus Mitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus in B. dothidea.
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【Molecular characterization of a novel mitovirus from the plant‑pathogenic fungus Botryosphaeria dothidea.].
【植物致病性真菌灰葡萄孢的新型线粒病毒的分子特征。】
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DOI：10.1007/s00705-020-04886-y文章类型：杂志文章
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：在这里，从一种植物病原性真菌Botryosphaeria dothidea中分离出了一种新型的分枝杆菌Bothosphaeria dothidea线粒体病毒1（BdMV1），并确定了其分子特性。 BdMV1具有2,667 nt的基因组，其中包含使用真菌线粒体遗传密码的单个大开放阅读框（ORF）。 ORF编码一个727个氨基酸的RNA依赖性RNA聚合酶（RdRp），分子量为81.64 kDa。 BLASTp分析显示，BdMV1的RdRp结构域分别与葡萄膜疟原虫相关的线粒体病毒43和粘粒线粒体线粒体病毒1具有39.59％和39.18％的序列同一性。系统发育分析进一步表明，BdMV1是线粒体病毒科中线粒体病毒属的新成员。据我们所知，这是首次在轮状芽胞杆菌中出现线粒体病毒的报道。
