The purpose of this study was to investigate the composition of throat microbiota in pulmonary tuberculosis patients (PTB) in comparison to healthy tuberculin skin test positive (TSTp) and negative (TSTn) individuals. Throat swabs samples were collected, and the microbiota was characterized. Richer operational taxonomic units (OTUs) were present in PTB group, compared to TSTp and TSTn. Regarding alpha diversity analysis there was a higher community diversity in TSTn compared to TSTp. Beta diversity analysis showed different species composition in TSTp compared to TSTn and PTB. There was higher presence of Firmicutes in PTB and TSTn compared to TSTp group at phylum level. At the genus level, Leuconostoc and Enterococcus were higher in TSTn compared to TSTp and Pediococcus, Chryseobacterium, Bifidobacterium, Butyrivibrio, and Bulleidia were higher in PTB compared to TSTn. Streptococcus was higher in TSTn compared to PTB and Lactobacillus in PTB compared to TSTp. At species level, Streptococcus sobrinus and Bulleidia moorei were higher in PTB compared to TSTn individuals, while Lactobacillus salivarius was higher in PTB compared to TSTp. The differences in the microbiome composition could influence the resistance/susceptibility to Mtb infection.
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【Microbial biodiversity in the throats of pulmonary tuberculosis patients and tuberculin skin test (TST) positive and negative healthy individuals in Malaysia.].
【马来西亚肺结核患者的喉咙中的微生物多样性和结核菌素皮肤试验（TST）阳性和阴性健康个体。】
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DOI：10.1016/j.tube.2020.101965文章类型：杂志文章
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：这项研究的目的是与健康结核菌素皮肤试验阳性（TSTp）和阴性（TSTn）个体相比，研究肺结核患者（PTB）的咽喉微生物群组成。收集咽拭子样品，并对微生物群进行鉴定。与TSTp和TSTn相比，PTB组中存在更丰富的操作分类单位（OTU）。关于α多样性分析，与TSTp相比，TSTn中的社区多样性更高。 Beta多样性分析显示，与TSTn和PTB相比，TSTp中的物种组成不同。在门生水平上，与TSTp组相比，PTB和TSTn中的Firmicutes含量更高。在属水平上，TSTn中的隐球菌和肠球菌比TSTp高，而Pediococcus，Chryseobacterium，Bifidobacterium，Butyrivibrio和Bulleidia在PTB中比TSTn高。与PTB相比，TSTn中的链球菌更高，而与TSTp相比，PTB中的乳酸菌更高。在物种水平上，与TSTn个体相比，PTB中的链球菌和莫氏肺炎链球菌较高，而与TSTp相比，唾液乳杆菌在PTB中则较高。微生物组组成的差异可能会影响对Mtb感染的抵抗力/敏感性。
