BACKGROUND & AIMS:
:Biochemical methane potential (BMP) assay was conducted at 35 °C to evaluate the effects of inoculum to substrate ratio (ISR) and substrate mix ratio between para-grass and pig manure co-digesting using different inocula. Rubber latex digester (RLD) inoculum showed higher methanogenic activity (41.4 mL CH4/gVS) than pig farm digester (PFD) inoculum (37.3 mL CH4/gVS). However, the maximum methane yields, occurred at the highest para-grass mix ratio (G), were 369.6, 437.6, 465.9 and 442.6 mL CH4/gTSadded for RLD inoculum, versus 332.4, 475.0, 519.5 and 521.9 mL/gTSadded for PFD inoculum at ISR 1, 2, 3, and 4, respectively. HPr, HBu and HVa appeared at higher G, corresponding to substrate's higher biodegradability. Response surface indicated that higher ISR and G had a significantly positive impact on methane yield. It suggested the use of higher ISR, i.e. 3 or 4, for BMP assay of these co-substrates. Dominant species of fermentative bacteria in each inoculum was tested by DGGE.
背景与目标:
:在35°C下进行生化甲烷潜力(BMP)分析,以评估接种物与底物比率(ISR)以及使用不同接种物共同消化副草和猪粪之间的底物混合比率的影响。橡胶乳胶消化器(RLD)接种物显示出更高的产甲烷活性(41.4 mL CH4 / gVS),高于养猪场消化器(PFD)接种物(37.3 mL CH4 / gVS)。然而,最大的甲烷产量出现在最高的草皮混合比(G)下,对于RLD接种物,添加的CH4 / gTS为369.6、437.6、465.9和442.6 mL,而对于PFD接种物,添加的甲烷为332.4、475.0、519.5和521.9 mL / gTS分别位于ISR 1、2、3和4。 HPr,HBu和HVa以较高的G出现,这对应于底物的较高的生物降解性。响应面表明,较高的ISR和G对甲烷产量具有明显的积极影响。它建议使用较高的ISR(即3或4)进行这些共底物的BMP测定。用DGGE检测每个接种物中的发酵菌的主要种类。