We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.

译文

我们描述了尿液中白蛋白的快速颗粒增强比浊法免疫测定。测定内和测定间CVs分别小于5% 和小于10%,检出限为2 mg/L,工作范围扩大至200 mg/L。添加到离心尿液中的白蛋白的平均分析回收率为100% (SD 10.6%),并且当将结果与通过Pharmacia RIA进行比较时,相关系数为0.99。工作试剂至少稳定六个月; 因此,该测定法适用于分批和紧急分析。

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