Olig2 protein, a member of the basic helix-loop-helix transcription factor family, was introduced into the mouse embryonic carcinoma cell line P19 for induction of motor neuron differentiation. We show that Olig2 protein has the ability to permeate the cell membrane without the addition of a protein transduction domain (PTD), similar to other basic helix-loop-helix transcription factors such as MyoD and NeuroD2. Motor neuron differentiation was evaluated for the elongation of neurites and the expression of choline acetyltransferase (ChAT) mRNA, a differentiation marker of motor neurons. By addition of Olig2 protein, motor neuron differentiation was induced in P19 cells.

译文

Olig2蛋白是基本螺旋-环-螺旋转录因子家族的成员,已被引入小鼠胚胎癌细胞系P19中,以诱导运动神经元分化。我们表明,Olig2蛋白具有在不添加蛋白质转导结构域 (PTD) 的情况下渗透细胞膜的能力,类似于其他基本螺旋-环-螺旋转录因子,例如MyoD和neurod2。评估运动神经元分化的神经突延伸和胆碱乙酰转移酶 (ChAT) mRNA (运动神经元的分化标记) 的表达。通过添加Olig2蛋白,在P19细胞中诱导运动神经元分化。

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