A haploid strain of Asp. nidulans with a chromosome segment in duplicate (one in normal position on chromosome I, one translocated to chromosome II) shows mitotic recombination, mostly by conversion, in adE in a frequency slightly higher than in the equivalent diploid. A method has been devised, using this duplication, for the selection of rec and uvs mutations. Six rec mutations have been found which decrease recombination frequency in the haploid. One mutation selected as UV sensitive showed a hundred fold increase in recombination frequency in the haploid (pop mutation) and probably the same in diploids. The increased frequency is both in gene conversion and in crossing over, and the exchanges appear in clusters of two or more. pop is allelic to uvsB (Jansen, 1970) which had been found to affect mitotic but not meiotic recombination. It is suggested that mutations of this type interfere with the control mechanism which determines that high recombination is confirmed to the meiotic nuclei and avoided in somatic nuclei.

译文

具有一式两份染色体片段的Asp.Nidulan的单倍体菌株 (一个在I号染色体上处于正常位置,一个易位为II号染色体) 在adE中显示有丝分裂重组,主要是通过转化,其频率略高于等效的二倍体。已经设计出一种使用这种重复的方法来选择rec和uvs突变。已发现六个rec突变降低了单倍体的重组频率。一个被选为对紫外线敏感的突变显示单倍体 (pop突变) 的重组频率增加了一百倍,而二倍体的重组频率可能相同。增加的频率既包括基因转化,也包括杂交,交换出现在两个或更多的簇中。pop与已发现影响有丝分裂但不影响减数分裂重组的uvsB (Jansen,1970) 等位。建议这种类型的突变会干扰控制机制,该机制决定了减数分裂核得到证实并避免了体细胞核中的高重组。

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