Response surface methodology was employed to optimize mixed substrate solid state fermentation for the production of cellulases and xylanase by Aspergillus fumigatus ABK9. Among 11 different parameters, fermentation time (86-88 h), medium pH (6.1-6.2), substrate amount (10.0-10.5 g) and substrate ratio (wheat bran:rice straw) (1.1) had significantly influences on enzyme production. Under these conditions endoglucanase, β-glucosidase, FPase (filter paper degrading activity) and xylanase activities of 826.2, 255.16, 102.5 and 1130.4 U/g, respectively were obtained. The enzyme cocktail extracted (solid to water ratio of 1:10) from the ferments increased brightness of waste office paper pulp by 82.8% ISO, Ink(D) value by 82.1%, removed chromophores (2.53 OD; A(237)nm) and hydrophobic compounds (1.15 OD; A(465)nm) and also decreased the kappa number to 13.5 from 16.8.

译文

采用响应面方法优化混合底物固态发酵,以通过烟曲霉abk9生产纤维素酶和木聚糖酶。在11个不同参数中,发酵时间 (86-88 h),培养基pH (6.1-6.2),底物量 (10.0-10.5g) 和底物比 (麦麸: 稻草) (1.1) 对酶的产生有显着影响。在这些条件下,分别获得826.2、255.16、102.5和1130.4 U/g的内切葡聚糖酶、 β-葡萄糖苷酶、FPase (滤纸降解活性) 和木聚糖酶活性。从发酵中提取的酶混合物 (1:10的固水比) 增加了废办公纸浆的亮度82.8% ISO,油墨 (D) 值82.1%,去除了发色团 (2.53 OD; A(237)nm) 和疏水性化合物 (1.15 OD; A(465)nm),并且还将kappa数从16.8降低至13.5。

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