• 【携带牛免疫缺陷病毒gag基因的嵌合BHIV(BIV / HIV-1)病毒的构建和鉴定。】 复制标题 收藏 收藏
    DOI:10.3748/wjg.v11.i17.2609 复制DOI
    作者列表:Zhu YX,Liu C,Liu XL,Qiao WT,Chen QM,Zeng Y,Geng YQ
    BACKGROUND & AIMS: AIM:To explore the possibility of the replacement of the gag gene between human immunodeficiency virus and bovine immunodeficiency virus, to achieve chimeric virions, and thereby gain a new kind of AIDS vaccine based on BHIV chimeric viruses. METHODS:A series of chimeric BHIV proviral DNAs differing in the replacement regions in gag gene were constructed, and then were transfected into 293T cells. The expression of chimeric viral genes was detected at the RNA and protein level. The supernatant of 293T cell was ultra centrifuged to detect the probable chimeric virion. Once the chimeric virion was detected, its biological activities were also assayed by infecting HIV-sensitive MT4 cells. RESULTS:Four chimeric BHIV proviral DNAs were constructed. Genes in chimeric viruses expressed correctly in transfected 293T cells. All four constructs assembled chimeric virions with different degrees of efficiency. These virions had complete structures common to retroviruses and packaged genomic RNAs, but the cleavages of the precursor Gag proteins were abnormal to some extent. Three of these virions tested could attach and enter into MT4 cells, and one of them could complete the course of reverse transcription. Yet none of them could replicate in MT4 cells. CONCLUSION:The replacement of partial gag gene of HIV with BIV gag gene is feasible. Genes in chimeric BHIVs are accurately expressed, and virions are assembled. These chimeric BHIVs (proviral DNA together with virus particles) have the potential to become a new kind of HIV/AIDS vaccine.
    背景与目标: 目的:探讨在人免疫缺陷病毒和牛免疫缺陷病毒之间替换gag基因的可能性,获得嵌合病毒体,从而获得一种新型的基于BHIV嵌合病毒的艾滋病疫苗。
    方法:构建了一系列gag基因置换区不同的嵌合BHIV原病毒DNA,然后转染到293T细胞中。在RNA和蛋白质水平检测到嵌合病毒基因的表达。将293T细胞的上清液超速离心以检测可能的嵌合病毒体。一旦检测到嵌合病毒体,就可以通过感染HIV敏感的MT4细胞来测定其生物学活性。
    结果:构建了4个嵌合的BHIV原病毒DNA。嵌合病毒中的基因在转染的293T细胞中正确表达。所有四个构建体以不同程度的效率组装了嵌合病毒体。这些病毒体具有逆转录病毒和包装的基因组RNA共有的完整结构,但前体Gag蛋白的切割在一定程度上异常。这些被测试的病毒粒子中的三个可以附着并进入MT4细胞,其中之一可以完成逆转录过程。但是它们都不能在MT4细胞中复制。
    结论:用BIV gag基因替代HIV的部分gag基因是可行的。嵌合BHIVs中的基因可以准确表达,并且可以组装病毒体。这些嵌合的BHIV(附加DNA和病毒颗粒)有可能成为新型HIV / AIDS疫苗。
  • 【BIV TAR RNA-Tat肽相互作用的热力学和溶剂化动力学。】 复制标题 收藏 收藏
    DOI:10.1039/c2mb25357g 复制DOI
    作者列表:Goel T,Kumar S,Maiti S
    BACKGROUND & AIMS: :The pulmonary hydatid cyst is frequent in Mediterranean countries such as Morocco. Our analytic study concerned 70 cases of lung hydatid cysts collected from 2007 to 2010. Mean age was 35years and we noted a male predominance (53%). Forty-seven percent of patients belong to rural environment where 64% of them were in contact with dogs. The respiratory symptomatology was made mostly by cough (86%) and chest pain (70%). Diagnosis was based on radioclinical arguments with positive hydatic serology in some cases. The cyst was single in 84% of the cases, safe in 55% of the cases. The location in the right lung was dominant with a major affection of the right lower lobe. Conventional surgery was indicated in 67 cases. The liver hydatid cyst was discovered in 20% of cases and treated at the same time phases in 71% of cases. The evolution was good in 73% of the cases and marked by a recurrence in three of the operated cases.
    背景与目标: :在摩洛哥等地中海国家,肺包虫囊肿很常见。我们的分析研究涉及2007年至2010年收集的70例肺包虫囊肿。平均年龄为35岁,我们注意到男性占多数(53%)。 47%的患者属于乡村环境,其中64%的人与狗接触。呼吸系统症状主要由咳嗽(86%)和胸痛(70%)引起。在某些情况下,诊断是基于放射学论证,且血清学阳性呈阳性。在84%的病例中,囊肿是单个的,在55%的病例中,囊肿是安全的。右肺的位置占优势,右下叶受累较大。 67例行常规手术。在20%的病例中发现了肝包虫囊肿,在71%的病例中同时进行了分期治疗。 73%的病例进展良好,其中三个手术病例复发。
  • 【牛免疫缺陷病毒长期存留在实验感染BIV(R29)分离株的牛中的抗原和遗传稳定性。】 复制标题 收藏 收藏
    DOI:10.1099/0022-1317-81-6-1463 复制DOI
    作者列表:Carpenter S,Vaughn EM,Yang J,Baccam P,Roth JA,Wannemuehler Y
    BACKGROUND & AIMS: :Experimental infection of cattle with bovine immunodeficiency virus (BIV) is characterized by persistent, low levels of virus replication in the absence of clinical disease. A virus neutralization (VN) assay was developed to examine the role of VN antibodies in controlling virus replication in cattle experimentally infected with the BIV(R29) isolate of BIV. All animals developed VN antibody, but there was no correlation between VN titres and restriction of virus replication in vivo. BIV infection did not induce high-titred, cross-neutralizing antibody and there was no evidence for antigenic variation through more than 4 years in vivo. Genetic comparisons among the BIV(R29) inoculum virus and viruses isolated from infected animals identified only limited genetic variation during 4 years in vivo. Moreover, there was no evidence that the observed variation was due to selection. Analyses of genetic diversity in the virus stock used for inoculation indicated a fairly homogeneous population. In the absence of high levels of virus replication and overt clinical disease, there appeared to be little selection of virus variants, resulting in antigenic and genetic stability of BIV(R29) during long-term, persistent infection.
    背景与目标: :牛免疫缺陷病毒(BIV)的实验性感染特征是在没有临床疾病的情况下持续低水平的病毒复制。开发了一种病毒中和(VN)分析方法,以检查VN抗体在控制BIV(R29)BIV(B29)分离株感染的牛中控制病毒复制中的作用。所有动物均产生VN抗体,但VN滴度与体内病毒复制限制之间没有相关性。 BIV感染不会诱导高滴度,交叉中和的抗体,并且在体内超过4年没有证据表明抗原发生变异。 BIV(R29)接种病毒和从感染动物中分离出的病毒之间的遗传比较发现,在体内4年内仅发现了有限的遗传变异。此外,没有证据表明观察到的变异是由于选择。用于接种的病毒原种的遗传多样性分析表明种群相当均一。在没有高水平的病毒复制和明显的临床疾病的情况下,似乎很少选择病毒变体,从而导致BIV(R29)在长期持续感染中的抗原和遗传稳定性。
  • 【Bi-shRNA STMN1 BIV在难治性癌症中的1期试验。】 复制标题 收藏 收藏
    DOI:10.1038/mt.2015.14 复制DOI
    作者列表:Barve M,Wang Z,Kumar P,Jay CM,Luo X,Bedell C,Mennel RG,Wallraven G,Brunicardi FC,Senzer N,Nemunaitis J,Rao DD
    BACKGROUND & AIMS:
    背景与目标:
  • 【TrpE融合蛋白在BIV包膜蛋白中鉴定抗原结构域的用途。】 复制标题 收藏 收藏
    DOI:10.1016/0166-0934(94)90029-9 复制DOI
    作者列表:Chen P,Liu ZQ,Wood C
    BACKGROUND & AIMS:
    背景与目标:
  • 【BIV TAR-Tat的灵活性:肽结合模型。】 复制标题 收藏 收藏
    DOI:10.1080/07391102.2002.10506840 复制DOI
    作者列表:Hsieh M,Collins ED,Blomquist T,Lustig B
    BACKGROUND & AIMS:
    背景与目标:
  • 【具有免疫反应性的His-BIV衣壳融合蛋白的过表达和纯化。】 复制标题 收藏 收藏
    DOI:10.1006/prep.1998.1004 复制DOI
    作者列表:Betemps D,Mallet F,Cheynet V,Baron T
    BACKGROUND & AIMS: :The gene of the capsid protein of bovine immunodeficiency virus (BIV) was linked to a sequence encoding for six histidines and expressed as the (His)6 p26 capsid fusion protein. The fusion protein was strongly expressed as both soluble and insoluble forms after induction by isopropylthio-beta-d-galactoside. Purification was based on interaction of the hexa-histidine polypeptide with metal ions. Expression could represent 11% of the total protein in Escherichia coli, allowing more than 20 mg of highly purified protein to be obtained per liter of bacterial culture. The (His)6 p26 capsid fusion protein purified by immobilized metal affinity chromatography reacted specifically in Western blot with sera from cattle experimentally infected by BIV, as well as with two monoclonal antibodies directed against different epitopes of the Gag protein. The ease of expression, purification, and specificity of this fusion protein should permit a thorough study of prevalence of BIV infection in large-scale serological studies of field samples.
    背景与目标: 牛免疫缺陷病毒(BIV)衣壳蛋白的基因与编码六个组氨酸的序列连接,并表示为(His)6 p26衣壳融合蛋白。在异丙基硫代-β-d-半乳糖苷诱导后,融合蛋白以可溶和不可溶形式强烈表达。纯化基于六组氨酸多肽与金属离子的相互作用。表达可代表大肠杆菌中总蛋白的11%,每升细菌培养物可获得超过20 mg的高度纯化的蛋白。通过固定的金属亲和色谱纯化的(His)6 p26衣壳融合蛋白在Western blot中与BIV实验感染的牛血清以及两种针对Gag蛋白不同表位的单克隆抗体发生特异性反应。这种融合蛋白的表达,纯化和特​​异性的简便性应允许在野外样品的大规模血清学研究中彻底研究BIV感染的患病率。
  • 【序列数据分析揭示了LSR2,模拟M. Leprae的重组融合蛋白与牛免疫缺陷病毒(BIV)的VIF之间的关系。】 复制标题 收藏 收藏
    DOI:10.1080/07391102.1997.10508970 复制DOI
    作者列表:Ramakrishnan S,Sukhaswami MB,Patil KM,Eswaran C
    BACKGROUND & AIMS:
    背景与目标:
  • 【BIV Tat肽对其TAR RNA双链体,RNA-DNA异源双链体和DNA双链体的结合研究。】 复制标题 收藏 收藏
    DOI:10.1016/j.bmcl.2004.10.030 复制DOI
    作者列表:Tok JB,Bi L
    BACKGROUND & AIMS:
    背景与目标:
  • 【牛ISG15:BIV感染的胎牛肺细胞中的一种抗病毒和可诱导蛋白。】 复制标题 收藏 收藏
    DOI:10.1186/1743-422X-7-134 复制DOI
    作者列表:Liu C,Li X,Yao X,Kong X,Qiao W,Geng Y
    BACKGROUND & AIMS:
    背景与目标:
  • 【在实验性感染和自然感染的牛中,牛慢病毒BIV的表面包膜基因的第二个高变区内的大小变化。】 复制标题 收藏 收藏
    DOI:10.1128/JVI.71.3.2482-2486.1997 复制DOI
    作者列表:Suarez DL,Whetstone CA
    BACKGROUND & AIMS:
    背景与目标:
  • 【牛免疫缺陷样病毒(BIV)在实验感染的小牛中具有转录活性。】 复制标题 收藏 收藏
    DOI:10.1007/BF01322672 复制DOI
    作者列表:Baron T,Mallet F,Polack B,Betemps D,Belli P
    BACKGROUND & AIMS:
    背景与目标:
  • 【石竹的挥发性成分。来自西西里岛:针对影响纤维素物体的微生物的活性。】 复制标题 收藏 收藏
    DOI:10.1080/14786419.2014.945087 复制DOI
    作者列表:Casiglia S,Bruno M,Senatore F
    BACKGROUND & AIMS: :Dianthus rupicola Biv. (cliffs carnation) is a camephytic, suffruticous, perennial plant growing up to 40 cm high. The plant is widespread in Sicily and neighbouring islands (Egadi, Lampedusa, Lipari) and in some areas of southern Italy. GC and GC-MS analyses of the essential oil distilled from the flowers showed the presence of 66 components. Its composition is characterised by the high content of thymol and carvacrol derivatives. A good antibacterial activity against Bacillus cereus and Bacillussubtilis, both infesting cellulosic historical material, was shown, whereas the antioxidant capacity was determined to be quite poor.
    背景与目标: :石竹(Dianthus rupicola) (崖石竹康乃馨)是一种杂草,杂种,多年生植物,长到40厘米高。该植物广泛分布在西西里岛和邻近的岛屿(埃加迪,兰佩杜萨,利帕里)和意大利南部的某些地区。从花朵中蒸馏出的精油的GC和GC-MS分析表明,存在66种成分。其组成特征在于百里酚和香芹酚衍生物含量高。结果表明,对侵染纤维素历史物质的蜡样芽孢杆菌和枯草芽孢杆菌具有良好的抗菌活性,而抗氧化能力却很差。
  • 14 Tool for BiV capture diagnosis. 复制标题 收藏 收藏

    【BiV捕获诊断工具。】 复制标题 收藏 收藏
    DOI:10.1111/pace.12448 复制DOI
    作者列表:Cacko A,Tataj E,Kocon M,Grabowski M
    BACKGROUND & AIMS:
    背景与目标:
  • 【Jacaranone:千里光歧义亚种的细胞毒性成分。歧义(直流)对抗肾腺癌ACHN和前列腺癌LNCaP细胞。】 复制标题 收藏 收藏
    DOI:10.1007/BF02977631 复制DOI
    作者列表:Loizzo MR,Tundis R,Statti GA,Menichini F
    BACKGROUND & AIMS: :Senecio ambiguus subsp. ambiguus (Biv.) DC. extracts were able to inhibit the in vitro proliferation of renal cell adenocarcinoma ACHN and hormone dependent prostate carcinoma LNCaP. The potential cytotoxic property of the plant was revealed by the methanolic extract action against LNCaP (IC50 of 5.51 microg/mL) and ACHN (IC50 of 38.95 microg/mL). The most potent cytotoxic activity (IC50 of 5.34 microg/mL against the prostate carcinoma cell line) was exerted by the dichloromethane extract. Through bioassay-guided fractionation of the dichloromethane extract jacaranone was isolated as the major active constituent. This quinoid showed a very strong activity against ACHN and LNcaP with IC50 of 4.32 and 7.39 microg/mL, respectively. Its structure was established by GC/MS and NMR analysis. The n-hexane extract showed an interesting inhibition on the proliferation of tumor cell lines an IC50 value of 5.23 microg/mL against LNCaP. Three compounds identified in the n-hexane extract such as nerolidol, a-humulene and g-tocopherol were found to be active aginst LNCAP with IC50 values ranged from 11.24 to 15.56 microg/mL.
    背景与目标: :千里光歧义亚种ambiguus(Biv。)DC。提取物能够抑制肾细胞腺癌ACHN和激素依赖性前列腺癌LNCaP的体外增殖。甲醇提取物对LNCaP(IC50为5.51 microg / mL)和ACHN(IC50为38.95 microg / mL)的作用表明了植物潜在的细胞毒性。二氯甲烷提取物发挥了最有效的细胞毒活性(对前列腺癌细胞系的IC50为5.34微克/毫升)。通过生物测定指导下的二氯甲烷萃取物分离,分离了以主要活性成分为成分的jacaranone。该醌类化合物对ACHN和LNcaP表现出非常强的活性,IC50分别为4.32和7.39 microg / mL。通过GC / MS和NMR分析确定其结构。正己烷提取物对肿瘤细胞系的增殖表现出有趣的抑制作用,对LNCaP的IC50值为5.23 microg / mL。发现在正己烷提取物中鉴定出的三种化合物(如橙皮醇,α-腐草烯和g-生育酚)是活性的LNCAP,IC50值为11.24至15.56 microg / mL。

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